Veröffentlichungen Molekulare Biotechnologie

Knödler, M., Opdensteinen, P., & Buyel, J. F.
Production of enzymes for the removal of odorous substances in plant biomass (2024) Protein Expression and Purification, 214, 106379. DOI

Mäckelmann, S., Känel, A., Kösters, L.M., Lyko, P., Prüfer, D., Noll, G.A., Wicke, S.
Gene complementation analysis indicates that parasitic dodder plants do not depend on the host FT protein for flowering. (2024) Plant Communications DOI

Roelfs, KU., Känel, A., Twyman, R.M., Prüfer, D., Schulze Gronover, C.
Epigenetic variation in early and late flowering plants of the rubber-producing Russian dandelion Taraxacum koksaghyz provides insights into the regulation of flowering time (2024).Sci Rep 14, 4283  DOI

Schmidt, F.J., Grundmann, L., Lahme, M., Seidemann, M., Schwarze, A., Lichtenauer, S., Twyman, R.M., Prüfer, D., Noll, G.A.
COL2-dependent photoperiodic floral induction in Nicotiana sylvestris seems to be lost in the N. sylvestris × N. tomentosiformis hybrid N. tabacum (2024) Frontiers in Plant Science DOI

 

Böttner, L, Malacrinò, A, Schulze Gronover, C, van Deenen, N, Müller, B, Xu, S, Gershenzon, J, Prüfer, D and Huber, M
Natural rubber reduces herbivory and alters the microbiome below ground (2023) New Phytologist DOI

Emonts, J., & Buyel, J. F.
An overview of descriptors to capture protein properties – tools and perspectives in the context of QSAR modeling (2023) Computational and Structural Biotechnology Journal, 21, 3234-3247. DOI

Gupta, M.D., Flaskamp, Y., Roentgen, R., Juergens, H., Armero-Gimenez, J., Albrecht, F., Hemmerich, J., Arfi, Z.A., Neuser, J., Spiegel, H., Schillberg, S., Yeliseev, A., Song, L., Qiu, J., Williams, C., Finnern, R.
Scaling eukaryotic cell-free protein synthesis achieved with the versatile and high-yielding tobacco BY-2 cell lysate (2023) Biotechnology and Bioengineering Jun 28. DOI

Knödler, M., Frank, K., Kerpen, L., & Buyel, J. F.
Design, optimization, production and activity testing of recombinant immunotoxins expressed in plants and plant cells for the treatment of monocytic leukemia (2023) Bioengineered, 14 (1), 2244235. DOI 

Knödler, M., Opdensteinen, P., Sankaranarayanan, R. A., Morgenroth, A., Buhl, E. M., Mottaghy, F. M., & Buyel, J. F.
Simple plant-based production and purification of the assembled human ferritin heavy chain as a nanocarrier for tumor-targeted drug delivery and bioimaging in cancer therapy (2023) Biotechnology and Bioengineering, 120(4), 1038-1054. DOI

Knödler, M., Reunious, P. W., & Buyel, J. F.
Risk assessment and bioburden evaluation of agrobacterium tumefaciens-mediated transient protein expression in plants using the CaMV35S promoter (2023) BMC Biotechnology, 23(1). DOI

Kottenhahn, P., Philipps, G., Bunk, B., Spröer, C., & Jennewein, S. (2023). The Restriction–Modification Systems of Clostridium carboxidivorans P7. Microorganisms, 11(12), 2962. DOI

Kouhen, M., García-Caparrós, P., Twyman, R.M., Abdelly, C., Mahmoudi, H., Schillberg, S., Debez, A. 
Insights from extremophile plants (2023) Critical Reviews in Biotechnology 43:4, 559-574. DOI

McElroy, C., Engels, B., Meusinger, R., & Jennewein, S. (2023). CYPArm2 is a CYP450 monooxygenase with protoilludene 13‐hydroxylase activity involved in the biosynthesis of armillyl orsellinate‐type sesquiterpenoids. ChemBioChem, e202300179.  DOI

Nausch, H., Baldan, M., Teichert, K., Lutz, J., Claussen, C., Bortz, M., & Buyel, J. F.
Simulation and optimization of nutrient uptake and biomass formation using a multi-parameter Monod-type model of tobacco BY-2 cell suspension cultures in a stirred-tank bioreactor (2023) Frontiers in Plant Science, 14. DOI

Ridgley, L. A., Falci Finardi, N., Gengenbach, B. B., Opdensteinen, P., Croxford, Z., Ma, J. K. C., Bodman-Smith, M., Buyel, J.F.,The, A. Y. H.
Killer to cure: Expression and production costs calculation of tobacco plant-made cancer-immune checkpoint inhibitors (2023) Plant Biotechnology Journal, 21(6), 1254-1269. DOI

Riekötter, J, Oklestkova, J, Muth, J, Twyman, RM., Epping, J
Transcriptomic analysis of Chinese yam (Dioscorea polystachya Turcz.) variants indicates brassinosteroid involvement in tuber development (2023) Frontiers Nutrition DOI

Wolters SM, Benninghaus VA, Roelfs KU, van Deenen N, Twyman RM, Prüfer D, Schulze Gronover C
Overexpression of a pseudo-etiolated-in-light-like protein in Taraxacum koksaghyz leads to a pale green phenotype and enables transcriptome-based network analysis of photomorphogenesis and isoprenoid biosynthesis (2023) Frontiers in Plant Science. DOI

 

 

 

Bernau, C. R., Knödler, M., Emonts, J., Jäpel, R. C., & Buyel, J. F.
The use of predictive models to develop chromatography-based purification processes (2022) Frontiers in Bioengineering and Biotechnology, 10. DOI

Biancacci, I., De Lorenzi, F., Theek, B., Bai, X., May, J. -., Consolino, L., Baues, M., Moeckel, D., Gremse, F., von Stillfried, S., El Shafei, A., Benderski, K., Azadkhah Shalmani, A., Wang, A., Momoh, J., Peña, Q., Buhl, E.M., Buyel, J.F., . . . Shi, Y.
Monitoring EPR effect dynamics during nanotaxane treatment with theranostic polymeric micelles (2022) Advanced Science, 9(10). DOI

Buntru, M., Hahnengress, N., Croon, A., Schillberg, S.
Plant-derived cell-free biofactories for the production of secondary metabolites (2022) Frontiers in Plant Science 12:794999. DOI

Buntru, M., Vogel, S., Finnern, R., Schillberg, S.
Plant-based cell-free transcription and translation of recombinant proteins (2022) Methods in Molecular Biology, 2480, 113-124. DOI

Buyel, J. F., Benvenuto, E., & Meyers, A. E.
Editorial: Proceedings of the 4th biennial conference of the international society for plant molecular farming (2022) Frontiers in Bioengineering and Biotechnology, 10. DOI

Buyel, J.F.
Statistical designs to improve downstream processing (2022) Methods in Molecular Biology, 2480, 295-310. DOI

Buyel, J.F.
Strategies for efficient and sustainable protein extraction and purification from pPlant tissues (2022) Methods in Molecular Biology, 2480, 127-145. DOI

Frank, K., Bernau, C.R., Buyel, J.F.
Spherical nanoparticles can be used as non-penetrating tracers to determine the extra-particle void volume in packed-bed chromatography columns (2022) Journal of Chromatography A, 1675, 463174. DOI

Hou, H. -., Bishop, C. A., Huckauf, J., Broer, I., Klaus, S., Nausch, H., & Buyel, J. F.
Seed- and leaf-based expression of FGF21-transferrin fusion proteins for oral delivery and treatment of non-alcoholic steatohepatitis (2022) Frontiers in Plant Science, 13. DOI 

Jäpel, R. C., & Buyel, J. F.
Bayesian optimization using multiple directional objective functions allows the rapid inverse fitting of parameters for chromatography simulations (2022) Journal of Chromatography A, 1679. DOI

Kalischuk, M., Müller, B., Fusaro, A.F., Wijekoon, C.P., Waterhouse, P.M., Prüfer, D., Kawchuk, L. 
Amplification of cell signaling and disease resistance by an immunity receptor Ve1Ve2 heterocomplex in plants (2022) Communications Biology, 5:497. DOI

Känel, P., Noll, G.A., Schroedter, K., Naffin, E., Kronenberg, J., Busswinkel, F., Twyman, R.M., Klämbt, C., Prüfer, D.
The tobacco phosphatidylethanolamine-binding protein NtFT4 increases the lifespan of Drosophila melanogaster by interacting with the proteostasis network (2022) Aging DOI

Lauer, I., Philipps, G., Jennewein, S.
Metabolic engineering of Clostridium ljungdahlii for the production of hexanol and butanol from CO2 and H2 (2022) Microbial Cell Factories, 21(1), 85. DOI

Mandal, M.K., Kronenberger, T., Zulka, M.I., Windshügel, B., Schiermeyer, A.
Structure-based discovery of small molecules improving stability of human broadly-neutralizing anti-HIV antibody 2F5 in plant suspension cells (2022) Biotechnology Journal, 17(4), 2100266. DOI

Mann, M., Effert, D., Kottenhahn, P., Hüser, A., Philipps, G., Jennewein, S., Büchs, J.
Impact of different trace elements on metabolic routes during heterotrophic growth of C. ljungdahlii investigated through online measurement of the carbon dioxide transfer rate (2022) Biotechnology Progress. DOI

Noll, G.A., Furch, A.C., Rose, J., Visser, F., Prüfer, D.
Guardians of the phloem–forisomes and beyond (2022) New Phytologist. DOI

Opdensteinen, P., Buyel, J.F.
Reducing water uptake into BY-2 cells by systematically optimizing the cultivation parameters increases product yields achieved by transient expression in plant cell packs (2022) Biotechnology Journal. DOI 

Opdensteinen, P., Sperl, L. E., Mohamadi, M., Kündgen-Redding, N., Hagn, F., & Buyel, J. F.
The transient expression of recombinant proteins in plant cell packs facilitates stable isotope labelling for NMR spectroscopy (2022) Plant Biotechnology Journal, 20(10), 1928-1939. DOI

Rose, J., Brand, I., Bilstein-Schloemer, M., Jachimska, B., Twyman, R.M., Prüfer, D., Noll, G.A.
The Ca2+ response of a smart forisome protein is dependent on polymerization
(2022) Protein Science, 31(3), 602 DOI

Schiermeyer, A., Cerda-Bennasser, P., Schmelter, T., Haung, X., Christou, P., Schillberg, S.
Rapid production of SaCas9 in plant-based cell-free lysate for activity testing (2022) Biotechnology Journal, 17(7), 2100564. DOI

Sheybanifard, M., Guerzoni, L. P. B., Omidinia-Anarkoli, A., De Laporte, L., Buyel, J.F., Besseling, R., . . . Metselaar, J. M. (2022).
Liposome manufacturing under continuous flow conditions: Towards a fully integrated set-up with in-line control of critical quality attributes (2022) Lab on a Chip, 23(1), 182-194. DOI

Schillberg, S., Spiegel, H. (eds.)
Recombinant protein production in plants: A brief overview of strengths and challenges (2022) Methods in Molecular Biology, 2480, 1-13. DOI

Schnieder. S., Känel, A., Zimmermann, M., Kriebs, K., Witte, A., Wrobel, L.S., Twyman, R.M., Prüfer, D., Furch, A.C.U., Noll, G.A.
So similar yet so different: The distinct contributions of extrafascicular and fascicular phloem to transport and exudation in cucumber plants (2022) Journal of Plant Physiology, 153643 DOI

Spiegel, H., Nölke, G., Thangaraj, H., Schillberg, S. 
The concept of an agroinfiltration kit for recombinant protein production for educational and commercial use – a journey through a forest of regulatory and legal implications (2022) Frontiers in Bioengineering and Biotechnology 10:926239.  DOI

Spiegel, H.
Improving recombinant protein recovery from plant tissue using heat precipitation (2022) Methods in Molecular Biology, 2480, 147-157. DOI

Spiegel, H., Schillberg, S., Nölke, G.
Production of recombinant proteins by agrobacterium-mediated transient expression (2022) Methods in Molecular Biology, 2480, 89-102. https://doi.org/10.1007/978-1-0716-2241-4_6 

Steinmaßl, M., Boudaden, J., Edgue, G., Freund, L.J., Meyer, S., Mordehay N., Soto, M., Endres, H.-E., Muth, J., Prüfer, D., Lerch, W., Kutter, C.
Passivated impedimetric sensors for immobilization-free pathogen detection by isothermal amplification and melt curve analysis
(2022) Biosensors, 12(5):261. DOI

Wieghaus, A., Roelfs, K.-U., Twyman, R.,M., Prüfer, D., Schulze Gronover, C.
Comparative transcriptome analysis in Taraxacum koksaghyz to identify genes that determine root volume and root length (2022) Frontiers in Genetics DOI

Ansari, S., Mousavi, A., Safarnejad, M.R., Farrokkhi, N., Alavi, S.M., Schillberg, S., Nölke, G.
Selection and characterization of two monoclonal antibodies specific for the Aspergillus flavus major antigenic cell wall protein Aflmp1 (2021) Fungal Biology 25(8), 621-629. DOI

Augustine, S.M., Vadakan Cherian, A., Seiling, K., DiFiore, S., Raven, N., Commandeur, U., Schillberg, S. (2021)
Targeted mutagenesis in Nicotiana tabacum ADF gene using shockwave-mediated ribonucleoprotein delivery increases osmotic stress tolerance (2021) Physiologica Plantarum 173(3), 993-1007. DOI

Bernau, C. R., Weiske, J., Nölting, S., Hübbers, J., Jäpel, R., Buyel, J. F.
Separation of tobacco host cell proteins for directed process development (2021) Jourmal pf Chromatography A. DOI

Buyel, J. F., Stöger, E., Bortesi, L.
Targeted genome editing of plants and plant cells for biomanufacturing (2021) Transgenic Research. DOI

Christou, P., Dhingra, A., Slamet-Loedin, H.I., Oliveira, M., Chakraborty, S., Buyel, J.F., Stoger, E., Schillberg, S., Orzaez, D., Quemada, H.
Preface: Genome editing in plants (2021) Transgenic Research 30, 317–320. DOI

Engels, B., Heinig, U., McElroy, C., Meusinger, R., Grothe, T., Stadler, M., Jennewein, S.
Isolation of a gene cluster from Armillaria gallica for the synthesis of armillyl orsellinate–type sesquiterpenoids (2021) Applied Microbiology and Biotechnology, 105(1), 211-224. DOI

Grundmann L., Känel A., Muth J., Beinecke F., Jekat M., Shen Y., Kudithipudi C., Xu D., Yang J., Warek U., Strickland J., Prüfer D., Noll G.A.
Tissue-specific expression of barnase in tobacco delays axillary shoot development after topping
(2021) Plant Biotechnology Journal DOI

He, W., Baysal, C., Lobato Gómez, M., Huang, X., Alvarez, D., Zhu, C., Armario-Najera, V., Blanco Perera, A., Cerda Bennaser, P., Saba-Mayoral, A., Sobrino-Mengual, G., Vargheese, A., Abranches, R., Abreu, I.A., Balamurugan, S., Bock, R., Buyel, J.F., da Cunha, N.B., Daniell, H., Faller, R., Folgado, A., Gowtham, I., Häkkinen, S.T., Kumar, S,, Sathish Kumar, R., Lacorte, C., Lomonossoff, G.P., Luís, I.M., Ma, J.K.C., McDonald, K.A., Murad, A., Nandi, S., O’Keef, B., Parthiban, S., Paul, M.J., Ponndorf, D., Rech, E., Rodrigues, J.C.M., Ruf, S., Schillberg, S., Schwestka, J., Shah, P.S., Singh, R., Stoger, E., Twyman, R.M., Varghese, I.P., Vianna, G.R., Webster, G., Wilbers, R.H.P., Christou, P., Oksman-Caldentey, K.M., Capell, T.
Contributions of the international plant science community to the fight against infectious diseases in humans – part 2: affordable drugs in edible plants for endemic and re-emerging diseases (2021) Plant Biotechnology Journal 19, 1921–1936. DOI

Hübbers, J., Buyel, J. F.
On the verge of the market – Plant factories for the automated and standardized production of biopharmaceuticals (2021) Biotechnology Advances. DOI

Ilić Đurđić, K., Ostafe, R., Prodanović, O., Đelmaš, A.Đ., Popović, N., Fischer, R., Schillberg, S., Prodanović, R.
Improved degradation of azo dyes by lignin peroxidase following mutagenesis at two sites near the catalytic pocket and the application of peroxidase-coated yeast cell walls (2021) Frontiers of Environmental Science & Engineering 15, 19. DOI

Janke, C.
Clostridia as a sustainable platform for chemicals and foods | [Clostriden als nachhaltige Plattform für Chemikalien und Lebensmittel] (2021) BioSpektrum, 27(5), 554-556. DOI

Knödler, M., Buyel, J. F.
Plant-made immunotoxin building blocks: A roadmap for producing therapeutic antibody-toxin fusions (2021) Biotechnology Advances. DOI

Kottenhahn, P., Philipps, G., Jennewein, S.
Hexanol biosynthesis from syngas by Clostridium carboxidivorans P7–product toxicity, temperature dependence and in situ extraction (2021) Heliyon, 7(8), e07732. DOI

Kronenberg, J., Schrödter, K., Noll, G.A., Twyman, R.M., Prüfer, D., Känel, P.
The tobacco phosphatidylethanolamine-binding protein NtFT4 simultaneously improves vitality, growth, and protein yield in human cells (2021) Biotechnology and Bioengineering, 118 (10), 3770-3786. DOI

Laibach N., Müller B., Pleissner D., Raber W., Smetana S.
An integrated, modular biorefinery for the treatment of food waste in urban areas
(2021) Case Studies in Chemical and Environmental Engineering, 4, DOI

Lidzba, N., García Arteaga, V., Schiermeyer, A., Havenith, H., Muranyi, I., Schillberg, S., Lehmann, J., Ueberham, E.
Development of monoclonal antibodies against pea globulins for multiplex assays targeting legume proteins (2021) Journal of Agricultural Food and Chemistry 69, 9, 2864-2874. DOI

Lobato Gómez, M., Huang, X., Alvarez, D., He, W., Baysal, C., Zhu, C., Armario-Najera, V., Blanco Perera, A., Cerda Bennasser, P., Saba-Mayoral, A., Sobrino-Mengual, G., Vargheese, A., Abranches, R., Abreu, I.A., Balamurugan, S., Bock, R., Buyel, J.F., da Cunha, N.B., Daniell, H., Faller, R., Folgado, A., Gowtham, I., Häkkinen, S.T., Kumar, S., Sathish Kumar, R., Lacorte, C., Lomonossoff, G.P., Luís, I.M., Ma, J.K.C., McDonald, K.A., Murad, A., Nandi, S., O’Keef, B., Oksman-Caldentey, K.M., Parthiban, S., Paul, M.J., Ponndorf, D., Rech, E., Rodrigues, J.C.M., Ruf, S., Schillberg, S., Schwestka, J., Shah, P.S., Singh, R., Stoger, E., Twyman, R.M., Varghese, I.P., Vianna, G.R., Webster, G., Wilbers, R.H.P., Capell, T., Christou, P.
Contributions of the international plant science community to the fight against human infectious diseases – part 1: epidemic and pandemic diseases (2021) Plant Biotechnology Journal 19, 1901–1920. DOI

Nausch H., Buyel J. F.
Cryopreservation of plant cell cultures – Current practices and protocols (2021). New Biotechnology. DOI

Nölke, G., Chudobova, I., Houdelet, M., Volke, D., Lusso, M., Frederick, J., Kudithipudi, C., Shen, Y., Warek, U., Strickland, J.A., Xu, D., Schinkel, H., Schillberg, S.
Impact of nicotine pathway downregulation on polyamine biosynthesis and leaf ripening in tobacco (2021) Plant Direct 5:e00329. DOI

Opdensteinen, P., Lobanov, A., Buyel, J. F.
Characterization of a combined pH and heat precipitation of tobacco host cell proteins for simplified downstream processing (2021) Biotechnology Journal. DOI

Opdensteinen, P., Meyer, S., Buyel, J. F.
Nicotiana spp. for the expression and purification of functional IgG3 antibodies directed against the Staphylococcus aureus alpha toxin (2021) Frontiers in Chemical Engineering. DOI

Parschat, K., Melsaether, C., Jäpelt, K. R., Jennewein, S.
Clinical evaluation of 16-week supplementation with 5HMO-Mix in healthy-term human infants to determine tolerability, safety, and effect on growth (2021) Nutrients, 13(8), 2871. DOI

Rose J., Brand I., Bilstein-Schloemer M., Jachimska B., Twyman R.M., Prüfer D., Noll G.A.
The Ca2+ response of a smart forisome protein is dependent on polymerization (2021) Protein Science, DOI

Rose, J., Müller, B., Groscurth, S., Giese, J., Eirich, J., Finkemeier, I., Twyman, R.M., Prüfer, D., Noll, G.A.
The functionality of plant mechanoproteins (forisomes) is dependent on the dual role of conserved cysteine residues (2021) International Journal of Biological Macromolecules DOI

Solís J.L., Muth J., Canales J., Lizana C., Prüfer D., Riegel R., Behn A.
Allelic diversity of three anthocyanin synthesis genes in accessions of native Solanum tuberosum L. ssp. tuberosum at the Potato Genebank of the Universidad Austral de Chile (2021) Genetic Resources and Crop Evolution, DOI

Schiermeyer, A., Cerda-Bennasser, P., Schmelter, T., Huang, X., Christou, P., Schillberg, S.
Rapid production of SaCas9 in plant-based cell-free lysate for activity testing (2021) Authorea. DOI

Schillberg, S., Finnern, R.
Plant molecular farming for the production of valuable proteins – critical evaluation of achievements and future challenges (2021) Journal of Plant Physiology 258-259, 153359. DOI

Vasilev, H., Šmejkal, K., Gronover, C.S., Choi, Y.H., Prüfer, D., Jankovská, D., Ionkova, I.
Flavonol glycosides from aerial parts of Astragalus thracicus Griseb (2021) Phytochemistry Letters, 41, 119-122. DOI

Wollenweber, T.E., van Deenen, N., Roelfs, K.-U., Prüfer, D., Gronover, C.S.
Microscopic and transcriptomic analysis of pollination processes in self-incompatible Taraxacum koksaghyz (2021) Plants, 10 (3), art. no. 555,1-17. DOI

 

 

 

 

 

Bröker, J. N., Müller, B., Prüfer, D., Schulze Gronover, C.
Combinatorial metabolic engineering in
Saccharomyces cerevisiae for the enhanced production of the FPP-derived sesquiterpene germacrene (2020) Bioengineering 7(4), 135. https://doi.org/10.3390/bioengineering7040135

Benninghaus, V.A., Van Deenen, N., Müller, B., Roelfs, K.-U., Lassowskat, I., Finkemeier, I., Prüfer, D., Gronover, C.S.
Comparative proteome and metabolome analyses of latex-exuding and non-exuding Taraxacum koksaghyz roots provide insights into laticifer biology (2020) Journal of Experimental Botany, 71 (4), 1278-1293. https://doi.org/10.1093/jxb/erz512

Capell, T., Twyman, R.M., Armario-Najera, V., Ma, J.K.-C., Schillberg, S., Christou, P. Potential Applications of Plant Biotechnology against SARS-CoV-2(2020) Trends in Plant Science. https://doi.org/10.1016/j.tplants.2020.04.009

Derya, S.M., Spiegel, H., Hanisch, F.-G., Morozov, V., Schroten, H., Jennewein, S., Parschat, K.
Biotechnologically produced fucosylated oligosaccharides inhibit the binding of human noroviruses to their natural receptors (2020) Journal of Biotechnology, 318, pp. 31-38. https://doi.org/10.1016/j.jbiotec.2020.05.001

Edgü, G., Freund, L.J., Hartje, S., Tacke, E., Hofferbert, H.-R., Twyman, R.M., Noll, G.A., Muth, J., Prüfer, D.
Fast, precise, and reliable multiplex detection of potato viruses by loop-mediated isothermal amplification (2020) International Journal of Molecular Sciences, 21 (22), 8741: 1-19. https://doi.org/10.3390/ijms21228741

Ilić Đurđić, K., Ece, S., Ostafe, R., Vogel, S., Balaž, A.M., Schillberg, S., Fischer, R., Prodanović, R.
Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability (2020) Journal of Bioscience and Bioengineering, 129 (6),664-671. https://doi.org/ 10.1016/j.jbiosc.2019.12.009

Ilić Đurđić, K., Ece, S., Ostafe, R., Vogel, S., Schillberg, S., Fischer, R., Prodanović, R.
Improvement in oxidative stability of versatile peroxidase by flow cytometry-based high-throughput screening system (2020) Biochemical Engineering Journal, 157, 107555. https://doi.org/ 10.1016/j.bej.2020.107555

Ilić Đurđić, K., Ostafe, R., Đurđević Đelmaš, A., Popović, N., Schillberg, S., Fischer, R., Prodanović, R.
Saturation mutagenesis to improve the degradation of azo dyes by versatile peroxidase and application in form of VP-coated yeast cell walls (2020) Enzyme and Microbial Technology, 136, e109509. https://doi.org/10.1016/j.enzmictec.2020.109509

Fischer, R., Buyel, J.F.
Molecular farming – The slope of enlightenment(2020) Biotechnology Advances, 40, art. no. 107519. https://doi.org/10.1016/j.biotechadv.2020.107519

Gengenbach, B.B., Opdensteinen, P., Buyel, J.F.
Robot Cookies – Plant Cell Packs as an Automated High-Throughput Screening Platform Based on Transient Expression (2020) Frontiers in Bioengineering and Biotechnology, 8, art. no. 393. https://doi.org/10.3389/fbioe.2020.00393

Janke, C., Gaida, S., Jennewein S.
The production of isoprene from cellulose using recombinant Clostridium cellulolyticum strains expressing isoprene synthase (2020) MicrobiologyOpen https://doi.org/10.1000/mbo3.1008

Kirchhoff, J., Schiermeyer, A., Schneider, K., Fischer, R., Ainley, W.M., Webb, S.R., Schinkel, H, Schillberg, S.
Gene expression variability between randomly and targeted transgene integration events in tobacco suspension cell lines (2020) Plant Biotechnol Rep https://doi.org/10.1007/s11816-020-00624-7

Pietschmann, J., Spiegel, H., Krause, H.-J., Schillberg, S., Schröper, F.
Sensitive Aflatoxin B1 Detection Using Nanoparticle-Based Competitive Magnetic Immunodetection (2020) Toxins, 12 (5). https://doi.org/10.3390/toxins12050337

Rose, J., Visser, F., Müller, B., Senft, M., Groscurth, S., Sicking, K.F., Twyman, R.M., Prüfer, D., Noll, G.A.
Identification and molecular analysis of interaction sites in the MtSEO-F1 protein involved in forisome assembly (2020) International Journal of Biological Macromolecules, 144, 603-614. https://doi.org/10.1016/j.ijbiomac.2019.12.092

Schiermeyer, A.
Optimizing product quality in molecular farming (2020) Current Opinion in Biotechnology, 61, 15-20. https://doi.org/10.1016/j.copbio.2019.08.012

Schmidt, F., Zimmermann, M., Lichtenauer, S., Wiedmann, D., Grundmann, L., Muth, J, Twyman, R, Prüfer, D, Noll, G
The Major Floral Promoter NtFT5 in tobacco (Nicotiana tabacum) is a promising target for crop improvement (2020) Front. Plant Sci. 10:1666. https://doi.org/10.3389/fpls.2019.01666

A - C

Achtsnicht, S., Neuendorf, C., Faßbender, T., Nölke, G., Offenhäusser, A., Krause, H.-J., Schröper, F.
Sensitive and rapid detection of cholera toxin subunit B using magnetic frequency mixing detection (2019) PLoS ONE, 14 (7), e0219356. http://doi.org/10.1371/journal.pone.0219356

Achtsnicht, S., Tödter, J., Niehues, J., Telöken, M., Offenhäusser, A., Krause, H.-J., Schröper, F.
3D printed modular immunofiltration columns for frequency mixing-based multiplex magnetic immunodetection (2019) Sensors (Switzerland), 19 (1), 148. http://doi.org/10.3390/s19010148

Arcalis, E., Ibl, V., Hilscher, J., Rademacher, T., Avesani, L., Morandini, F., Bortesi, L., Pezzotti, M., Vitale, A., Pum, D., De Meyer, T., Depicker, A., Stoger, E.
Russell-like bodies in plant seeds share common features with prolamin bodies and occur upon recombinant protein production (2019) Frontiers in Plant Science, 10, 777. https://doi.org/10.3389/fpls.2019.00777

Buyel, J.F.
Hitze stabilisiert Malariaimpfstoffkandidaten in komplexen Proteinextrakten (2019) BioSpektrum, 25 (2), 163-166. http://doi.org/10.1007/s12268-019-1026-x

Buyel, J.F.
Plant molecular farming – Integration and exploitation of side streams to achieve sustainable biomanufacturing (2019) Frontiers in Plant Science, 9, 1893. http://doi.org/10.3389/fpls.2018.01893

Conrady, M., Lemoine, A., Limberg, M.H., Oldiges, M., Neubauer, P., Junne, S.
Carboxylic acid consumption and production by Corynebacterium glutamicum (2019) Biotechnology Progress, e2804. http://doi.org/10.1002/btpr.2804

 

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Gengenbach, B.B., Keil, L.L., Opdensteinen, P., Müschen, C.R., Melmer, G., Lentzen, H., Bührmann, J., Buyel, J.F.
Comparison of microbial and transient expression (tobacco plants and plant-cell packs) for the production and purification of the anticancer mistletoe lectin viscumin (2019) Biotechnology and Bioengineering, 116 (9), 2236-2249. http://doi.org/10.1002/bit.27076

Hussain, A.F., Heppenstall, P.A., Kampmeier, F., Meinhold-Heerlein, I., Barth, S.
One-step site-specific antibody fragment auto-conjugation using SNAP-tag technology (2019) Nature Protocols, 14, 3101-3125. http://doi.org/10.1038/s41596-019-0214-y

Jansing, J., Buyel, J.F.
The Correlation Between DsRed mRNA Levels and Transient DsRed Protein Expression in Plants Depends on Leaf Age and the 5' Untranslated Region (2019) Biotechnology Journal 14 (3), 1800075. http://doi.org/10.1002/biot.201800075

Jansing, J., Schiermeyer, A., Schillberg, S., Fischer, R., Bortesi, L.
Genome editing in agriculture: Technical and practical considerations (2019) International Journal of Molecular Sciences, 20 (12), 2888. http://doi.org/10.3390/ijms20122888

Kern, K., Havenith, H., Delaroque, N., Rautenberger, P., Lehmann, J., Fischer, M., Spiegel, H., Schillberg, S., Ehrentreich-Foerster, E., Aurich, S., Treudler, R., Szardenings, M.
The immunome of soy bean allergy: Comprehensive identifi­cation and characterization of epitopes (2019) Clinical and Experi­mental Allergy, 49 (2), 239-251. http://doi.org/10.1111/cea.13285

Knödler, M., Rühl, C., Emonts, J., Buyel, J. F.
Seasonal weather changes affect the yield and quality of recombinant proteins produced in transgenic tobacco plants in a greenhouse setting (2019) Frontiers in Plant Science, 10, 1245. http://doi.org/10.3389/fpls.2019.01245

Knödler, M., Rühl C., Opdensteinen, P., Buyel, J.F.
Activated cross-linked agarose for the rapid development of affinity chromatography resins – Antibody capture as a case study (2019) Journal of Visualized Experiments, 150, e59933. http://doi.org/10.3791/59933

Kovačević, G., Ostafe, R., Fischer, R., Prodanović, R.
Influence of methionine residue position on oxidative stability of glucose oxidase from Aspergillus niger (2019) Biochemical Engineering Journal, 146, 143-149. http://doi.org/10.1016/j.bej.2019.03.016


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Maennling, A.E., Tur, M.K., Niebert, M., Klockenbring, T., Zeppernick, F., Gattenlöhner, S., Meinhold-Heerlein, I., Hussain, A.F.
Molecular targeting therapy against egfr family in breast cancer: Progress and future potentials (2019) Cancers, 11 (12), e1826. https://doi.org/10.3390/cancers11121826

Niephaus, E., Müller, B., van Deenen, N., Lassowskat, I., Bonin, M., Finkemeier, I., Prüfer, D., Schulze Gronover, C.
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Nölke, G., Barsoum, M., Houdelet, M., Arcalis, E., Kreuzaler, F., Fischer, R., Schillberg, S.
The integration of algal carbon concentration mechanism components into tobacco chloroplasts increases photosynthetic efficiency and biomass (2019). Biotechnology Journal, 14 (3), 1800170. http://doi.org/10.1002/biot.201800170

Opdensteinen, P., Clodt, J.I., Müschen, C.R., Filiz, V., Buyel, J.F.
A combined ultrafiltration/diafiltration step facilitates the purification of cyanovirin-n from transgenic tobacco extracts (2019) Frontiers in Bioengineering and Biotechnology, 6, 206. http://doi.org/10.3389/fbioe.2018.00206

Philipps, G., De Vries, S., Jennewein, S.
Development of a metabolic pathway transfer and genomic integration system for the syngas-fermenting bacterium Clostridium ljungdahlii (2019) Biotechnology for Biofuels, 12 (1), 112. http://doi.org/10.1186/s13068-019-1448-1

Pütter, K.M., van Deenen, N., Müller, B., Fuchs, L., Vorwerk, K., Unland, K., Bröker, J.N., Scherer, E., Huber, C., Eisenreich, W., Prüfer, D., Schulze Gronover, C.
The enzymes OSC1 and CYP716A263 produce a high variety of triterpenoids in the latex of Taraxacum koksaghyz (2019) Scientific Reports, 9 (1), 5942. http://doi.org/10.1038/s41598-019-42381-w

Rademacher, T., Sack, M., Blessing, D., Fischer, R., Holland, T., Buyel, J.
Plant cell packs: A scalable platform for recombinant protein production and metabolic engineering (2019) Plant Biotechnology Journal, 17 (8), 1560-1566. http://doi.org/10.1111/pbi.13081

Rasche S., Schillberg S., Derwenskus F., Schmid-Staiger U., Schließmann U.
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Santos, R.B., Pires, A.S., van der Hoorn, R.A.L., Schiermeyer, A., Abranches, R.
Generation of transgenic cell suspension cultures of the model legume Medicago truncatula: a rapid method for Agrobacterium mediated gene transfer (2019) Plant Cell, Tissue and Organ Culture, 136 (3), 445-450. http://doi.org/10.1007/s11240-018-1525-3

Schiermeyer, A., Schneider, K, Kirchhoff, J., Schmelter, T., Koch, N, Ke, J., Herwartz, D., Blue, R., Marri, P., Samuel, P., Corbin, D.R., Webb, S.R., Gonzalez, D.O., Folkerts, O., Fischer, R., Schinkel, H., Ainley, W. M., Schillberg, S.
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Schillberg, S., Raven, N., Spiegel, H., Rasche, S., Buntru, M.
Critical analysis of the commercial potential of plants for the production of recombinant proteins (2019) Frontiers in Plant Science, 10, 720. http://doi.org/10.3389/fpls.2019.00720

Schmitz, C., Auza, L.G., Koberidze, D., Rasche, S., Fischer, R., Bortesi, L.
Conversion of chitin to defined chitosan oligomers: Current status and future prospects (2019) Marine Drugs, 17 (8), 452. http://doi.org/10.3390/md17080452

Schubert, M., Xue, S., Ebel, F., Vaggelas, A., Krylov, V.B., Nifantiev, N.E., Chudobová, I., Schillberg, S., Nölke, G.
Monoclonal antibody AP3 binds galactomannan antigens displayed by the pathogens Aspergillus flavus, A. fumigatus, and A. parasiticus (2019) Frontiers in Cellular and Infection Microbiology, 9, 234. http://doi.org/10.3389/fcimb.2019.00234

Spiegel, H., Boes, A., Perales Morales, C., Rademacher, T., Buyel, J.F.
Ready to use stocks of Agrobacterium tumefaciens can simplify process development for the production of recombinant proteins by transient expression in plants (2019) Biotechnology Journal, 1900113. http://doi.org/10.1002/biot.201900113

Ueberham, E., Spiegel, H., Havenith, H., Rautenberger, P., Lidzba, N., Schillberg, S., Lehmann, J.
Simplified tracking of a soy allergen in processed food using a monoclonal antibody-based sandwich ELISA targeting the soybean 2S Albumin Gly m 8 (2019) Journal of Agricultural and Food Chemistry, 67, 8660-8667. http://doi.org/10.1021/acs.jafc.9b02717

van Deenen, N., Unland, K., Prüfer, D., Schulze Gronover, C.
Oxidosqualene cyclase knock-down in latex of Taraxacum koksaghyz reduces triterpenes in roots and separated natural rubber (2019) Molecules, 24 (15), 2703. http://doi.org/10.3390/molecules24152703

Volke, D.C., Rohwer, J., Fischer, R., Jennewein, S.
Investigation of the methylerythritol 4-phosphate pathway for microbial terpenoid production through metabolic control analysis (2019) Microbial Cell Factories, 18, 192. http://doi.org/10.1186/s12934-019-1235-5

Wieghaus, A., Prüfer, D., Schulze Gronover, C.
Loss of function mutation of the Rapid Alkalinization Factor (RALF1)-like peptide in the dandelion Taraxacum koksaghyz entails a high biomass taproot phenotype (2019) PLoS ONE, 14 (5), e0217454. http://doi.org/10.1371/journal.pone.0217454

Wu, D., von Roepenack-Lahaye, E., Buntru, M., de Lange, O., Schandry, N., Pérez-Quintero, A.L., Weinberg, Z., Lowe-Power, T.M., Szurek, B., Michael, A.J., Allen, C., Schillberg, S., Lahaye, T.
A pathogen effector subverts translational regulation to boost host polyamine levels (2019) Social Science Research Network. http://dx.doi.org/10.2139/ssrn.3376660

Zafarnia, S., Mrugalla, A., Rix, A., Doleschel, D., Gremse, F., Wolf, S.D., Buyel, J.F., Albrecht, U., Bode, J.G., Kiessling, F., Lederle, W.
Non-invasive imaging and modeling of liver regeneration after partial hepatectomy (2019) Frontiers in Physiology, 10, 904. http://doi.org/10.3389/fphys.2019.00904

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Beinecke, F.A., Grundmann, L., Wiedmann, D.R., Schmidt, F.J., Caesar, A.S., Zimmermann, M., Lahme, M., Twyman, R.M., Prüfer, D., Noll, G.A.
The FT/FD-dependent initiation of flowering under long-day conditions in the day-neutral species Nicotiana tabacum originates from the facultative short-day ancestor ­Nicotiana ­tomentosiformis (2018) Plant Journal, 96 (2), 329-342. http://doi.org/10.1111/tpj.14033

Bröker, J.N., Laibach, N., Müller, B., Prüfer, D., Schulze Gronover, C.
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Bröker, J.N., Müller, B., van Deenen, N., Prüfer, D., Schulze Gronover, C.
Upregulating the mevalonate pathway and repressing sterol synthesis in Saccharomyces cerevisiae enhances the production of triterpenes (2018) Applied Microbiology and Biotechnology 1-12 http://doi.org/DOI:10.1007/s00253-018-9154-7

Buyel, J.F.
Plants as sources of natural and recombinant anti-cancer agents (2018) Biotechnology Advances, 36 (2), 506-520. http://doi.org/10.1016/j.biotechadv.2018.02.002

Chouman, K., Korioth-Schmitz, B., Sack, M., Schmitz, J.E., Pham, A.T., Fischer, R., Barth, S., Klockenbring, T., Fendel, R.
Characterization of new anti-IL-6 antibodies revealed high potency candidates for intracellular cytokine detection and specific targeting of IL-6 receptor binding sites (2018) European Cytokine Network, 29 (2), 59-72. http://doi.org/10.1684/ecn.2018.0409

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Gengenbach, B.B., Müschen, C.R., Buyel J.F.
Expression and purification of human phosphatase and actin regulator 1 (PHACTR1) in plant-based systems (2018) Protein Expression and Purification, 151, 46-55 [Epub ahead of print] http://doi.org/10.1016/j.pep.2018.06.003

Gresch, G., Schenke, L., Mladenov, R., Zwirner, S., Cremer, C., Niesen, J., Grieger, E., Brümmendorf, T., Jost, E., Fischer, R., Stockmeyer, B., Barth, S., Nachreiner, T., Stein, C.
Elimination of different leukaemia subtypes using novel CD89-specific human cytolytic fusion proteins (2018) British ­Journal of Haematology, 183 (2), 313-317. http://doi.org/10.1111/bjh.14971

Hausmann, R., Chudobová, I., Spiegel, H., Schillberg, S.
Proteomic analysis of CHO cell lines producing high and low quantities of a recombinant antibody before and after selection with methotrexate (2018) Journal of Biotechnology, 265, 65-69. https://doi.org/10.1016/j.jbiotec.2017.11.008

Holland, T., Buyel, J.F. Bioreactor-based production of glycoproteins in plant cell suspension cultures (2018) Methods in Molecular Biology, 1674, 129-146. http://doi.org/10.1007/978-1-4939-7312-5_11
 

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Jansing, J., Buyel, J.F.
The Correlation Between DsRed mRNA Levels and Transient DsRed Protein Expression in Plants Depends on Leaf Age and the 5' Untranslated Region (2018) Biotechnology Journal [Epub ahead of print] http://doi.org/10.1002/biot.201800075

Kern, K., Havenith, H., Delaroque, N., Rautenberger, P., Lehmann, J., Fischer, M., Spiegel, H., Schillberg, S., Ehrentreich-Foerster, E., Aurich, S., Treudler, R., Szardenings, M.
The immunome of soy bean allergy: Comprehensive identifi­cation and characterization of epitopes (2018) Clinical and Experi­mental Allergy, e13285. http://doi.org/10.1111/cea.13285

Laibach, N., Schmidl, S., Müller, B., Bergmann, M., Prüfer, D., Schulze Gronover, C.
Small rubber particle proteins from Taraxacum brevicorniculatum promote stress tolerance and influence the size and distribution of lipid droplets and artificial poly(cis-1,4-isoprene) bodies (2018) Plant Journal, 93 (6), 1045-1061. http://doi.org/10.1111/tpj.13829

Lim, S.S.Y., Chua, K.H., Nölke, G., Spiegel, H., Goh, W.L., Chow, S.C., Kee, B.P., Fischer, R., Schillberg, S., Othman, R.Y.
Plant-derived chimeric antibodies inhibit the invasion of human fibroblasts by Toxoplasma gondii  (2018) PeerJ, 2018 (12), 5780. http://doi.org/10.7717/peerj.5780

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Martínez, M.E., Poirrier, P., Prüfer, D., Schulze, C., Jorquera, L., Ferrer, P., Díaz, K., Chamy, R.
Kinetics and modeling of cell growth for potential anthocyanin induction in cultures of Taraxacum officinale G.H. Weber ex Wiggers (Dandelion) in vitro (2018) Electronic Journal of Biotechnology, 36, 15-23. http://doi.org/10.1016/j.ejbt.2018.08.006

McElroy, C., & Jennewein, S. (2018). Taxol® biosynthesis and production: from forests to fermenters. In Biotechnology of Natural Products (pp. 145-185). Springer, Cham. http://doi.org/10.1007/978-3-319-67903-7

Menzel, S., Holland, T., Boes, A., Spiegel, H., Fischer, R., Buyel, J.F.
Downstream processing of a plant-derived malaria transmission-blocking vaccine candidate (2018) Protein Expression and Purification, 152, 122-130. http://doi.org/10.1016/j.pep.2018.07.012

Nölke, G., Barsoum, M., Houdelet, M., Arcalis, E., Kreuzaler, F., Fischer, R., Schillberg, S.
The integration of algal carbon concentration mechanism components into tobacco chloroplasts increases photosynthetic efficiency and biomass (2018). Biotechnology Journal, 1800170. https://doi.org/10.1002/biot.201800170

Rühl, C., Knödler, M., Opdensteinen, P., Buyel, J.F.
A linear epitope coupled to DsRed provides an affinity ligand for the capture of monoclonal antibodies (2018) Journal of Chromatography A, 1571, 55-64. http://doi.org/10.1016/j.chroma.2018.08.014
 

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Santos, R.B., Chandrasekar, B., Mandal, M.K., Kaschani, F., Kaiser, M., Both, L., van der Hoorn, R.A.L., Schiermeyer, A., Abranches, R.
Low Protease Content in Medicago truncatula Cell Cultures Facilitates Recombinant Protein Production (2018) Biotechnology Journal, 13 (7), 1800050. http://doi.org/10.1002/biot.201800050

Schubert, M., Spiegel, H., Schillberg, S., Nölke, G. Aspergillus-specific antibodies – Targets and applications (2018) Biotechnology Advances, 36 (4), 1167-1184. http://doi.org/10.1016/j.biotechadv.2018.03.016

Spiegel, H., Stöger, E., Twyman, R.M., Buyel, J.F.
Current status and perspectives of the molecular farming landscape (2018) Molecular Pharming: Applications, Challenges and Emerging Areas, 1-23. http://doi.org/10.1002/9781118801512.ch1

Tyutereva, E.V., Dobryakova, K.S., Schiermeyer, A., Shishova, M.F., Pawlowski, K., Demidchik, V., Reumann, S., Voitsekhovskaja, O.V. The levels of peroxisomal catalase protein and activity modulate the onset of cell death in tobacco BY-2 cells via reactive oxygen species levels and autophagy (2018) Functional Plant Biology, 45 (1-2), 247-258.http://doi.org/10.1071/FP16418

Wollschlaeger, C., Meinhold-Heerlein, I., Cong, X., Bräutigam, K., Di Fiore, S., Zeppernick, F., Klockenbring, T., Stickeler, E., Barth, S., Hussain, A.F.
Simultaneous and Independent Dual Site-Specific Self-Labeling of Recombinant Antibodies (2018) Bioconjugate Chemistry, 29 (11), 3586-3594. http://doi.org/10.1021/acs.bioconjchem.8b00545

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Akinrinmade, O.A., Jordaan, S., Hristodorov, D., Mladenov, R., Mungra, N., Chetty, S., Barth, S., 2017. Human MAP tau based targeted cytolytic fusion proteins. Biomedicines No. 5(3). http://doi.org/10.3390/biomedicines5030036

Augustine, S.M., 2017. CRISPR-cas9 system as a genome editing tool in sugarcane in: Mohan C. (Ed.) Sugarcane Biotechnology: Challenges and Prospects Springer International Publishing pp. 155-172. http://doi.org/10.1007/978-3-319-58946-6_11

Bauerschlag, D., Meinhold-Heerlein, I., Maass, N., Bleilevens, A., Bräutigam, K., Al Rawashdeh, W., Di Fiore, S., Haugg, A.M., Gremse, F., Steitz, J., Fischer, R., Stickeler, E., Barth, S., Hussain, A.F., 2017. Detection and Specific Elimination of EGFR+ Ovarian Cancer Cells Using a Near Infrared Photoimmunotheranostic Approach. Pharmaceutical Research 34, 696–703. http://doi.org/10.1007/s11095-017-2096-4

Berges, N., Arens, K., Kreusch, V., Fischer, R., Di Fiore, S., 2017. Toward discovery of novel microtubule targeting agents: A SNAP-tag-based high-content screening assay for the analysis of microtubule dynamics and cell cycle progression. SALAS Discovery 22, 387–398. http://doi.org/10.1177/2472555216685518

Braig, F., Kriegs, M., Voigtlaender, M., Habel, B., Grob, T., Biskup, K., Blanchard, V., Sack, M., Thalhammer, A., Batalla, I.B., Braren, I., Laban, S., Danielczyk, A., Goletz, S., Jakubowicz, E., Märkl, B., Trepel, M., Knecht, R., Riecken, K., Fehse, B., Loges, S., Bokemeyer, C., Binder, M., 2017. Cetuximab resistance in head and neck cancer is mediated by EGFR-K521 polymorphism. Cancer Research 77, 1188–1199. http://doi.org/10.1158/0008-5472.CAN-16-0754

Buyel, J.F., 2017a. Blanching facilitates the purification of recombinant proteins from plants. BioSpektrum 23, 522–524. http://doi.org/10.1007/s12268-017-0835-z

Buyel, J.F., 2017b. How plants can contribute to the supply of anticancer compounds, in: Biotechnology and Production of Anti-Cancer Compounds. pp. 39–72. http://doi.org/10.1007/978-3-319-53880-8_2

Buyel, J.F., Twyman, R.M., Fischer, R., 2017. Very-large-scale production of antibodies in plants: The biologization of manufacturing. Biotechnology Advances 35, 458–465. http://doi.org/10.1016/j.biotechadv.2017.03.011

Choudhary, S., Barth, S., Verma, R.S., 2017. SNAP-Tag Technology: A Promising Tool for Ex Vivo Immunophenotyping. Molecular Diagnosis and Therapy 21, 315–326. http://doi.org/10.1007/s40291-017-0263-2

Chouman, K., Woitok, M., Mladenov, R., Kessler, C., Weinhold, E., Hanz, G., Fischer, R., Meinhold-Heerlein, I., Bleilevens, A., Gresch, G., Haugg, A.M., Zeppernick, F., Bauerschlag, D., Maass, N., Stickeler, E., Kolberg, K., Hussain, A.F., 2017. Fine tuning antibody conjugation methods using SNAP-tag technology. Anti-Cancer Agents in Medicinal Chemistry 17, 1434–1440. http://doi.org/10.2174/1871520617666170213123737

Costa, M.P., Feitosa, A.C.S., Oliveira, F.C.E., Cavalcanti, B.C., Dias, G.G., Caetano, E.W.S., Sales, F.A.M., Freire, V.N., Di Fiore, S., Fischer, R., Ladeira, L.O., Da Silva Júnior, E.N., Pessoa, C., 2017. Encapsulation of nor-β-lapachone into poly(d,l)-lactide-: Co -glycolide (PLGA) microcapsules: Full characterization, computational details and cytotoxic activity against human cancer cell lines. MedChemComm 8, 1993–2002. http://doi.org/10.1039/c7md00196g

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Ece, S., Lambertz, C., Fischer, R., Commandeur, U., 2017. Heterologous expression of a Streptomyces cyaneus laccase for biomass modification applications. AMB Express 7. http://doi.org/10.1186/s13568-017-0387-0

Edgue, G., Twyman, R.M., Beiss, V., Fischer, R., Sack, M., 2017. Antibodies from plants for bionanomaterials. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology 9. http://doi.org/10.1002/wnan.1462

Fischer, R., Schillberg, S., 2017. Molecular Farming, in: Thomas, B., Murray, B.G., Murphy, D.J. (Eds.), Encyclopedia of Applied Plant Sciences (Second Edition). Academic Press, Oxford, pp. 77–82. http://doi.org/10.1016/B978-0-12-394807-6.00159-3

Gresch, G., Schenke, L., Mladenov, R., Zwirner, S., Cremer, C., Niesen, J., Grieger, E., Brümmendorf, T., Jost, E., Fischer, R., Stockmeyer, B., Barth, S., Nachreiner, T., Stein, C., 2017. Elimination of different leukaemia subtypes using novel CD89-specific human cytolytic fusion proteins. Br J Haematol n/a-n/a. http://doi.org/10.1111/bjh.14971

Grieger, E., Gresch, G., Niesen, J., Woitok, M., Barth, S., Fischer, R., Fendel, R., Stein, C., 2017. Efficient targeting of CD13 on cancer cells by the immunotoxin scFv13–ETA′ and the bispecific scFv [13xds16]. Journal of Cancer Research and Clinical Oncology 143, 2159–2170. http://doi.org/10.1007/s00432-017-2468-5

Havenith, H., Kern, K., Rautenberger, P., Spiegel, H., Szardenings, M., Ueberham, E., Lehmann, J., Buntru, M., Vogel, S., Treudler, R., Fischer, R., Schillberg, S., 2017. Combination of two epitope identification techniques enables the rational design of soy allergen Gly m 4 mutants. Biotechnology Journal 12. http://doi.org/10.1002/biot.201600441

Houdelet, M., Galinski, A., Holland, T., Wenzel, K., Schillberg, S., Buyel, J.F., 2017. Animal component-free Agrobacterium tumefaciens cultivation media for better GMP-compliance increases biomass yield and pharmaceutical protein expression in Nicotiana benthamiana. Biotechnology Journal 12. http://doi.org/10.1002/biot.201600721

Huck, N.V., Leissing, F., Majovsky, P., Buntru, M., Aretz, C., Flecken, M., Müller, J.P.J., Vogel, S., Schillberg, S., Hoehenwarter, W., Conrath, U., Beckers, G.J.M., 2017. Combined 15N-labeling and tandemmoac quantifies phosphorylation of map kinase substrates downstream of MKK7 in Arabidopsis. Frontiers in Plant Science No. 8. http://doi.org/10.3389/fpls.2017.02050

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Kastilan, R., Boes, A., Spiegel, H., Voepel, N., Chudobová, I., Hellwig, S., Buyel, J.F., Reimann, A., Fischer, R., 2017. Improvement of a fermentation process for the production of two PfAMA1-DiCo-based malaria vaccine candidates in Pichia pastoris. Scientific Reports 7. http://doi.org/10.1038/s41598-017-11819-4

Kessler, C., Pardo, A., Tur, M.K., Gattenlöhner, S., Fischer, R., Kolberg, K., Barth, S., 2017. Novel PSCA targeting scFv-fusion proteins for diagnosis and immunotherapy of prostate cancer. Journal of Cancer Research and Clinical Oncology 143, 2025–2038. http://doi.org/10.1007/s00432-017-2472-9

Klose, D., Woitok, M., Niesen, J., Beerli, R.R., Grawunder, U., Fischer, R., Barth, S., Fendel, R., Nachreiner, T.:
Generation of an artificial human B cell line test system using Transpo-mAbTM technology to evaluate the therapeutic efficacy of novel antigen-specific fusion proteins. PLoS ONE (2017) No. 12(7) http://doi.org/0.1371/journal.pone.0180305

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Purcell, O., Opdensteinen, P., Chen, W., Lowenhaupt, K., Brown, A., Hermann, M., Cao, J., Tenhaef, N., Kallweit, E., Kastilan, R., Sinskey, A.J., Perez-Pinera, P., Buyel, J.F., Lu, T.K., 2017. Production of Functional Anti-Ebola Antibodies in Pichia pastoris. ACS Synthetic Biology No. 6(12), 2183-2190. http://doi.org/10.1021/acssynbio.7b00234

Pütter, K.M., van Deenen, N., Unland, K., Prüfer, D., Schulze Gronover, C., 2017. Isoprenoid biosynthesis in dandelion latex is enhanced by the overexpression of three key enzymes involved in the mevalonate pathway. BMC Plant Biology 17. http://doi.org/10.1186/s12870-017-1036-0

Schillberg, S., Raven, N., Fischer, R., Twyman, R.M., Schiermeyer, A., 2017. Contained Molecular Farming Using Plant Cell and Tissue Cultures, in: Yoshida, T. (Ed.), Applied Bioengineering. Wiley-VCH Verlag GmbH & Co. KGaA, pp. 259–281. http://doi.org/10.1002/9783527800599.ch9

Spiegel, H., Boes, A., Fendel, R., Reimann, A., Schillberg, S., Fischer, R., 2017. Immunization with the malaria diversity-covering blood-stage vaccine candidate plasmodium falciparum apical membrane antigen 1 DiCo in complex with its natural ligand PfRon2 does not improve the in vitro efficacy. Frontiers in Immunology 8. http://doi.org/10.3389/fimmu.2017.00743

Stolze, A., Wanke, A., van Deenen, N., Geyer, R., Prüfer, D., Schulze Gronover, C., 2017. Development of rubber-enriched dandelion varieties by metabolic engineering of the inulin pathway. Plant Biotechnology Journal 15, 740–753. http://doi.org/10.1111/pbi.12672

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Woitok, M., Klose, D., Di Fiore, S., Richter, W., Stein, C., Gresch, G., Grieger, E., Barth, S., Fischer, R., Kolberg, K., Niesen, J., 2017. Comparison of a mouse and a novel human scFv-SNAP-auristatin F drug conjugate with potent activity against EGFR-overexpressing human solid tumor cells. OncoTargets and Therapy 10, 3313–3327. http://doi.org/10.2147/OTT.S140492

Zhu, C., Bortesi, L., Baysal, C., Twyman, R.M., Fischer, R., Capell, T., Schillberg, S., Christou, P., 2017. Characteristics of Genome Editing Mutations in Cereal Crops. Trends in Plant Science 22, 38–52. http://doi.org/10.1016/j.tplants.2016.08.009

Zischewski, J., Fischer, R., Bortesi, L., 2017. Detection of on-target and off-target mutations generated by CRISPR/Cas9 and other sequence-specific nucleases. Biotechnology Advances 35, 95–104. http://doi.org/10.1016/j.biotechadv.2016.12.003

 

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Addai-Mensah, O., Seidel, M., Amidu, N., Maskus, D.J., Kapelski, S., Breuer, G., Franken, C., Owusu-Dabo, E., Frempong, M., Rakotozandrindrainy, R., Schinkel, H., Reimann, A., Klockenbring, T., Barth, S., Fischer, R., Fendel, R.: Acquired immune responses to three malaria vaccine candidates and their relationship to invasion inhibition in two populations naturally exposed to malaria. Malaria Journal. Online Journal (2016) No. 15: Art.65 (http://doi.org/10.1186/s12936-016-1112-1 )

Amoury, M., Bauerschlag, D., Zeppernick, F., Felbert, V., Berges, N., Di Fiore, S.D., Mintert, I., Bleilevens, A., Maass, N., Bräutigam, K., Meinhold-Heerlein, I., Stickeler, E., Barth, S., Fischer, R., Hussain, A.F.: Photoimmunotheranostic agents for triple-negative breast cancer diagnosis and therapy that can be activated on demand. OncoTarget 7 (2016), No. 34: 54925-54936 (http://doi.org/10.18632/oncotarget.10705)

Amoury, M., Kolberg, K., Pham, A.-T., Hristodorov, D., Mladenov, R., Di Fiore, S., Helfrich, W., Kiessling, F., Fischer, R., Pardo, A., Thepen, T., Hussain, A.F., Nachreiner, T., Barth, S.: Granzyme B-based cytolytic fusion protein targeting EpCAM specifically kills triple negative breast cancer cells in vitro and inhibits tumor growth in a subcutaneous mouse tumor model. Cancer letters 372 (2016) No. 2: 201-209 (http://doi.org/10.1016/j.canlet.2016.01.027)

Amoury, M., Mladenov, R., Nachreiner, T., Pham, A.-T., Hristodorov, D., Di Fiore, S., Helfrich, W., Pardo, A., Fey, G., Schwenkert, M., Thepen, T., Kiessling, F., Hussain, A.F., Fischer, R., Kolberg, K., Barth, S.: A novel approach for targeted elimination of CSPG4-positive triple-negative breast cancer cells using a MAP tau-based fusion protein. International Journal of Cancer 139 (2016) No. 4: 916-927 (http://doi.org/10.1002/ijc.30119)

Arfi, Z.A., Hellwig, S., Drossard, J., Fischer, R., Buyel, J.F.: Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisCO depletion and the removal of endotoxins. Biotechnology Journal 11 (2016) No. 4: 507-518 (http://doi.org/10.1002/biot.201500271)

Baysal, C., Bortesi, L., Zhu, C., Farré, G., Schillberg, S., Christou, P.: CRISPR/Cas9 activity in the rice OsBEIIb gene does not induce off-target effects in the closely related paralog OsBEIIa. Molecular Breeding 36 (2016) No. 8 (http://doi.org/10.1007/s11032-016-0533-4)

Bialon, M., Grezella, C., Friesen, L., Sieben, T., Pham, A.T., Fischer, R., et al.: A monoclonal antibody that discriminates between SNAP-Tagged and CLIP-tagged proteins. Monoclonal antibodies in immunodiagnosis and immunotherapy. 2016 No. 35(3): 141-147 (http://dx.doi.org/10.1089%2Fmab.2016.0002)

Bodewein, L., Schmelter, F., Di Fiore, S., Hollert, H., Fischer, R., Fenske, M.: Differences in toxicity of anionic and cationic PAMAM and PPI dendrimers in zebrafish embryos and cancer cell lines. Toxicology and Applied Pharmacology (2016) No. 395: 83-92 (http://doi.org/10.1016/j.taap.2016.06.008)

Boes, A., Reimann, A., Twyman, R.M., Fischer, R., Schillberg, S., Spiegel, H.: A plant-based transient expression system for the rapid production of malaria vaccine candidates. Methods in Molecular Biology (2016) No. 1404: 597-619 (http://doi.org/10.1007/978-1-4939-3389-1_39)

Boes, A., Spiegel, H., Kastilan, R., Bethke, S., Voepel, N., Chudobova, I., Bolscher, J.M., Dechering, K.J., Fendel, R., Buyel, J.F., Reimann, A., Schillberg, S., Fischer, R.: Analysis of the dose-dependent stage-specific in vitro efficacy of a multi-stage malaria vaccine candidate cocktail. Malaria Journal. Online Journal 15 (2016) Art. 279 (http://doi.org/10.1186/s12936-016-1328-0)

Boesch, A.W., Osei-Owusu, N.Y., Crowley, A.R., Chu, T.H., Chan, Y.N., Weiner, J.A., Bharadwaj, P., Hards, R., Adamo, M.E., Gerber, S.A., Cocklin, S.L., Schmitz, J.E., Miles, A.R., Eckman, J.W., Belli, A.J., Reimann, K.A., Ackerman, M.E.: Biophysical and functional characterization of rhesus macaque IgG subclasses.  Front Immunology (2016) (http://doi.org/10.3389/fimmu.2016.00589)

Bortesi, L., Zhu, C., Zischewski, J., Perez, L., Bassié, L., Nadi, R., Forni, G., Lade, S.B., Soto, E., Jin, X., Medina, V., Villorbina, G., Munoz, P., Farré, G., Fischer, R., Twyman, R.M., Capell, T., Christou, P., Schillberg, S.: Patterns of CRISPR/Cas9 activity in plants, animals and microbes. Plant biotechnology journal 14 (2016) No.12: 2203-2216 (http://doi.org/10.1111/pbi.12634)

Buyel, J.F.: Numeric simulation can be used to predict heat transfer during the blanching of leaves and intact plants. Biochemical Engineering Journal (2016) No. 109: 118-126 (http://doi.org/10.1016/j.bej.2016.01.009)

Buyel, J.F.: Procedure to evaluate the efficiency of flocculants for the removal of dispersed particles from plant extracts. Journal of Visualized Experiments: JoVE. Online resource (2016) No.110: Art. e53940 (http://doi.org/10.3791/53940)

Buyel, J.F., Gruchow, H.M., Tödter, N., Wehner, M.: Determination of the thermal properties of leaves by non-invasive contact free laser probing. Journal of Biotechnology (2016) No. 217: 100-108 (http://dx.doi.org/10.1016%2Fj.jbiotec.2015.11.008)

Buyel, J.F., Hubbuch, J., Fischer, R.: Comparison of tobacco host cell protein removal methods by blanching intact plants or by heat treatment of extracts. Journal of Visualized Experiments: JoVE. Online resource (2016) No.114 (http://doi.org/10.3791/54343)

Cartwright, E.K., Spicer, L., Smith, S.A., Lee, D., Fast, R., Paganini, S., Lawson, B.O., Nega, M., Easley, K., Schmitz, J.E., Bosinger, S.E., Paiardini, M., Chahroudi, A., Vanderford, T.H., Estes, J.D., Lifson, J.D., Derdeyn, C.A., Silvestri, G.: CD8+ lymphocytes are required for maintaining viral suppression in SIV-infected macaques treated with short-term antiretroviral therapy. Immunity. (2016) No. 45(3): 656-668 (http://doi.org/10.1016/j.immuni.2016.08.018. PMID: 27653601)

Carvajal-Rondanelli, P., Aróstica, M., Marshall, S.H., Albericio, F., Álvarez, C.A., Ojeda, C., Aguilar, L.F., Guzmán, F.: Inhibitory effect of short cationic homopeptides against Gram-negative bacteria. Amino Acids (2016) No. 48(6): 1445-1456 (http://doi.org/10.1007/s00726-016-2198-z)

Castro, R.I., Forero-Doria, O., Guzmán, L., Laurie, V.F., Valdés, O., Ávila-Salas, F., López-Cortés, X., Santos, L.S.: New polymer for removal of wine phenolics: Poly(N-(3-(N-isobutyrylisobutyramido)-3-oxopropyl)acrylamide) (P-NIOA). Food Chemistry (2016) No. 213:  554-560 (http://dx.doi.org/10.1016%2Fj.foodchem.2016.06.060)

Chan, Y.N., Boesch, A.W., Osei-Owusu, N.Y., Emileh, A., Crowley, A.R., Cocklin, S.L., Finstad, S.L., Linde, C.H., Howell, R.A., Zentner, I., Cocklin, S., Miles, A.R., Eckman, J.W., Alter, G., Schmitz, J.E., Ackerman, M.E.: IgG binding characteristics of rhesus macaque FcgR. Journal of Immunology (2016) No. 197(7): 2936-2947 (http://doi.org/10.4049/jimmunol.1502252. PMID: 27559046)

Choudhary, S., Pardo, A., Rosinke, R., Batra, J.K., Barth, S. Verma, R.S.: Targeting c-kit receptor in neuroblastomas and colorectal cancers using stem cell factor (SCF)-based recombinant bacterial toxins. Applied Microbiology and Biotechnology (2016) No. 100(1): 263-277 (http://dx.doi.org/10.1007%2Fs00253-015-6978-2)

Cong, X., Cremer, C., Nachreiner, T., Barth, S., Carloni, P.: Engineered human angiogenin mutations in the placental ribonuclease inhibitor complex for anticancer therapy: Insights from enhanced sampling simulations. Protein Science : a publication of the Protein Society (2016) No. 25(8): 1451-1460 (http://dx.doi.org/10.1002%2Fpro.2941)

Costa, M.P., Feitosa, A.C.S., Oliveira, F.C.E., Cavalcanti, B.C., Da Silva, E.N., Jr., Dias, G.G., Sales, F.A.M., Sousa, B.L., Barroso-Neto, I.L., Pessoa, C., Caetano, E.W.S., Di Fiore, S., Fischer, R., Ladeira, L.O., Freire, V.N.: Controlled release of Nor-b-lapachone by PLGA Microparticles: a strategy for improving cytotoxicity against prostate cancer cells. Molecules 21 (2016) No. 7: 12 S. (http://doi.org/10.3390/molecules21070873)

 

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Damm, T., Spiegel, H., Barth, S., Fischer, R., Naehring, J.: Development of a competitive cystatin C-Specific bioassay suitable for repetitivem measurements. PloS one. (2016) No. 11(1): e0147177 (http://dx.doi.org/10.1371%2Fjournal.pone.0147177)

Fischer, R., Schulze Gronover, C., Prüfer, D: Naturkautschuk aus russischem Löwenzahn. Ressourceneffizienz, Schlüsseltechnologie für Wirtschaft&Gesellschaft. Fraunhofer Forschungsfokus, Hrsg. Reimund Neugebauer (2016) 232-329  

Fritsch, L., Soeur, R., Hansen, C., Fischer, R., Schillberg, S., Schröper, F.: Next-generation sequencing of amplicons is a rapid and reliable method for the detection of polymorphisms relevant for barley breeding. Molecular Breeding 36 (2016) No.83 (http://doi.org/10.1007/s11032-016-0507-6)

Gaida, S.M., Liedtke, A., Jentges, A.H.W., Engels, B., Jennewein, S.: Metabolic engineering of clostridium cellulolyticum for the production of n-butanol from crystalline cellulose. Microbial Cell Factories. Online journal 15 (2016), Art.6 (http://doi.org/10.1186/s12934-015-0406-2)

Hristodorov, D., Mladenov, R., Fischer, R., Barth, S., Thepen, T.: Fully human MAP-fusion protein selectively targets and eliminates proliferating CD64(+) M1 macrophages. Immunology and cell biology (2016) No. 94(5): 470-478 (http://dx.doi.org/10.1038%2Ficb.2016.4)

Huber, M., Epping, J., Schulze Gronover, C., Fricke, J., Aziz, Z., Brillatz, T., Swyers, M., Köllner, T.G., Vogel, H., Hammerbacher, A., Triebwasser-Freese, D., Robert, C.A.M., Verhoeven, K., Preite, V., Gershenzon, J., Erb, M.: A latex metabolite benefits plant fitness under root herbivore attack. PLoS Biology (2016) No. 14(1) (http://doi.org/10.1371/journal.pbio.100233)

 

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Keating, S.M., Heitman, J.W., Wu, S., Deng, X., Stacey, A.R., Zahn, R.C., de la Rosa, M., Finstad, S.L., Lifson, J.D., Piatak, M., Gauduin, M.C., Kessler, B.M., Ternette, N., Carville, A., Johnson, R.P., Desrosiers, R.C., Letvin, N.L., Borrow, P., Norris, P.J., Schmitz, J.E.: Magnitude and quality of cytokine and chemokine storm during acute infection distinguish non-progressive and progressive simian immunodeficiency virus infections of nonhuman primates. Journal of  Virology (2016) No. 90(22): 10339-10350 PMID: 27630228 (http://doi.org/10.1128/JVI.01061-16)

Klose, D., Saunders, U., Barth, S., Fischer, R., Jacobi, A.M., Nachreiner, T.: Novel fusion proteins for the antigen-specific staining and elimination of B cell receptor-positive cell populations demonstrated by a tetanus toxoid fragment C (TTC) model antigen. Bmc Biotechnology (2016) No. 16: 18 (http://dx.doi.org/10.1186%2Fs12896-016-0249-x)

Madeira, L.M., Szeto, T.H., Henquet, M., Raven, N., Runions, J., Huddleston, J., Garrard, I., Drake, P.M.W., Ma, J.K.-C.: High-yield production of a human monoclonal IgG by rhizosecretion in hydroponic tobacco cultures. Plant biotechnology journal 14 (2016) No. 2: 615-624 (http://doi.org/10.1111/pbi.12407)

Mandal, M.K., Ahvari, H., Schillberg, S., Schiermeyer, A.: Tackling unwanted proteolysis in plant production hosts used for molecular farming. Frontiers in Plant Science (2016) No. 7: Art. 267 (http://doi.org/10.3389/fpls.2016.00267)

Maskus, D.J., Królik, M., Bethke, S., Spiegel, H., Kapelski, S., Seidel, M., Addai-Mensah, O., Reimann, A., Klockenbring, T., Barth, S., Fischer, R., Fendel, R.: Characterization of a novel inhibitory human monoclonal antibody directed against Plasmodium falciparum apical membrane antigen 1. Scientific Reports (2016) No. 6: Art. 39462 (http://doi.org/10.1038/srep39462)

Menzel, S., Holland, T., Boes, A., Spiegel, H., et al.: Optimized blanching reduces the host cell protein content and substantially enhances the recovery and stability of two plant-derived malaria vaccine candidates. Frontiers in Plant Science (2016) No. 7: 1-15 (http://dx.doi.org/10.3389%2Ffpls.2016.00159)

Mladenov, R., Hristodorov, D., Cremer, C., Gresch, G., Grieger, E., Schenke, L., et al.: CD64-directed microtubule associated protein tau kills leukemic blasts ex vivo. Oncotarget. (2016) No. 7(41): 67166-67174 (http://doi.org/10.18632/oncotarget.11568)

Niesen, J., Hehmann-Titt, G., Woitok, M., Fendel, R., Barth, S., Fischer, R., et al.: A novel fully-human cytolytic fusion protein based on granzyme B shows in vitro cytotoxicity and ex vivo binding to solid tumors overexpressing the epidermal growth factor receptor. Cancer letters. (2016) No. 374(2): 229-240 (http://dx.doi.org/10.1016%2Fj.canlet.2016.02.020)

Niesen, J., Sack, M., Seidel, M., Fendel, R., Barth, S., Fischer, R., et al.: SNAP-tag technology: a useful tool to determine affinity constants and other functional parameters of novel antibody fragments. Bioconjugat Chemistry (2016) No. 27(8): 1931-1941 (http://doi.org/10.1021/acs.bioconjchem.6b00315)

 

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Peschen, D., Schillberg, S., Fischer, R.: Antibody-mediated pathogen resistance in plants. Methods in Molecular Biology (2016) No. 1385: 273-291 (http://doi.org/10.1007/978-1-4939-3289-4_19)

Rasche, S., Herwartz, D., Schuster, F., Jabloka, N., Weber, A., Fischer, R., Schillberg, S.: More for less: Improving the biomass yield of a pear cell suspension culture by design of experiments. Scientific Reports (2016) No.6 : 23371(http://doi.org/10.1038/srep23371)

Rasche, S., Schmitz, C., Jablonka, N., Schillberg, S.: LED-beleuchtung von Fußballrasen – Möglichkeiten, Chancen und Grenzen. European Journal of Turfgrass Science (2016) No. 4: 69–75 (http://doi.org/10.1109/pesa.2006.343093)

Raven, N., Schillberg, S., Rasche, S.: Plant cell-based recombinant antibody manufacturing with a 200 L orbitally shaken disposable bioreactor. Methods in Molecular Biology (2016) No. 1385: 161–172 (http://doi.org/10.1007/978-1-4939-3289-4_12)

Schinkel, H., Schillberg, S.: Genome editing: intellectual property and product development in plant biotechnology. Plant Cell Reports (2016) No. 7: 1487-1491(http://doi.org/10.1007/s00299-016-1988-9)

Schmitz, C., Fritsch, L., Fischer, R., Schillberg, S., Rasche, S.: Statistical experimental designs for the production of secondary metabolites in plant cell suspension cultures. Biotechnology letters 38 (2016) No.12: 2007-2014 (http://doi.org/10.1007/s10529-016-2206-0)

Schneider, K., Schiermeyer, A., Dolls, A., Koch, N., Herwartz, D., Kirchhoff, J., Fischer, R., Russell, S.M., Cao, Z., Corbin, D.R., Sastry-Dent, L., Ainley, W.M., Webb, S.R., Schinkel, H., Schillberg, S.: Targeted gene exchange in plant cells mediated by a zinc finger nuclease double cut. Plant Biotechnology Journal 14 (2016) No. 4: 1151-1160 (http://doi.org/10.1111/pbi.12483)

Stolze, A., Wanke, A., van Deenen, N., Geyer, R., Prüfer, R., Schulze Gronover, C.: Development of rubber-enriched dandelion varieties by metabolic engineering of the inulin pathway. Plant Biotechnology Journal (2016) (http://doi.org/10.1111/pbi.12672)

 

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Vasilev, N., Boccard, J., Lang, G., Grömping, U., Fischer, R., Goepfert, S., Rudaz, S., Schillberg, S.: Structured plant metabolomics for the simultaneous exploration of multiple factors. Scientific Reports (2016) No. 6: Art. 37390 (http://doi.org/10.1038/srep37390)

Vasilev, N., Smales, C.M., Fischer, R., Schillberg S., Schiermeyer, A. (2016): Developments in the production of mucosal antibodies in plants. Biotechnology Advances (2016) No. 34 (2): 77-87 (http://doi.org/10.1016/j.biotechadv.2015.11.002)

Visser, F., Müller, B., Rose, J., Prüfer, D., Noll, G.A.: Forizymes – functionalised artificial forisomes as a platform for the production and immobilisation of single enzymes and multienzyme complexes. Science Reports (2016) No. 6: 30839 (http://doi.org/10.1038/srep30839)

von Felbert, V., Bauerschlag, D., Maass, N., Brautigam, K., Meinhold-Heerlein, I., Woitok, M., et al.: A specific photoimmunotheranostics agent to detect and eliminate skin cancer cells expressing EGFR. Journal of Cancer Research and Clinical Oncology (2016) No. 142(5): 1003-1011 (http://doi.org/10.1007/s00432-016-2122-7)

Woitok, M., Klose, D., Niesen, J., Richter, W., Abbas, M., Stein, C., et al.: The efficient elimination of solid tumor cells by EGFR-specific and HER2-specific scFv-SNAP fusion proteins conjugated to benzylguanine-modified auristatin F. Cancer Letters. (2016) No. 381(2): 323-330 (http://doi.org/10.1016/j.canlet.2016.08.003)

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Beiss, V., Spiegel, H., Boes, A., Kapelski, S., Scheuermayer, M., Edgue, G., Sack, M., Fendel, R., Reimann, A., Schillberg, S., Pradel, G., Fischer, R., 2015a. Heat-precipitation allows the efficient purification of a functional plant-derived malaria transmission-blocking vaccine candidate fusion protein. Biotechnol. Bioeng. 112, 1297–1305. http://doi.org/10.1002/bit.25548

Beiss, V., Spiegel, H., Boes, A., Scheuermayer, M., Reimann, A., Schillberg, S., Fischer, R., 2015b. Plant expression and characterization of the transmission-blocking vaccine candidate PfGAP50. BMC Biotechnology 15, 108. http://doi.org/10.1186/s12896-015-0225-x

Boes, A., Spiegel, H., Edgue, G., Kapelski, S., Scheuermayer, M., Fendel, R., Remarque, E., Altmann, F., Maresch, D., Reimann, A., Pradel, G., Schillberg, S., Fischer, R., 2015a. Detailed functional characterization of glycosylated and nonglycosylated variants of malaria vaccine candidate PfAMA1 produced in Nicotiana benthamiana and analysis of growth inhibitory responses in rabbits. Plant Biotechnol J 13, 222–234. http://doi.org/10.1111/pbi.12255

Boes, A., Spiegel, H., Voepel, N., Edgue, G., Beiss, V., Kapelski, S., Fendel, R., Scheuermayer, M., Pradel, G., Bolscher, J.M., Behet, M.C., Dechering, K.J., Hermsen, C.C., Sauerwein, R.W., Schillberg, S., Reimann, A., Fischer, R., 2015b. Analysis of a Multi-component Multi-stage Malaria Vaccine Candidate--Tackling the Cocktail Challenge. PLoS ONE 10, e0131456. http://doi.org/10.1371/journal.pone.0131456

Bortesi, L., Fischer, R., 2015. The CRISPR/Cas9 system for plant genome editing and beyond. Biotechnology Advances 33, 41–52. http://doi.org/10.1016/j.biotechadv.2014.12.006

Brehm, H., Hristodorov, D., Pardo, A., Mladenov, R., Niesen, J., Fischer, R., Tur, M.K., Barth, S., 2015. Targeted killing of rhabdomyosarcoma cells by a MAP-based human cytolytic fusion protein. Cancer Lett. 365, 149–155. http://doi.org/10.1016/j.canlet.2015.04.004

Buntru, M., Vogel, S., Stoff, K., Spiegel, H., Schillberg, S., 2015. A versatile coupled cell-free transcription–translation system based on tobacco BY-2 cell lysates. Biotechnol. Bioeng. 112, 867–878. http://doi.org/10.1002/bit.25502

Buyel, J.F., 2015. Controlling the interplay between Agrobacterium tumefaciens and plants during the transient expression of proteins. Bioengineered 6, 242–244. http://doi.org/10.1080/21655979.2015.1052920

Buyel, J.F., 2015. Process Development Strategies in Plant Molecular Farming. Curr Pharm Biotechnol 16, 966–982. http://doi.org/10.2174/138920101611150902115413

Buyel, J.F., Buyel, J.J., Haase, C., Fischer, R., 2015a. The impact of Pseudomonas syringae type III effectors on transient protein expression in tobacco. Plant Biol (Stuttg) 17, 484–492. http://doi.org/10.1111/plb.12264

Buyel, J.F., Gruchow, H.M., Fischer, R., 2015. Depth Filters Containing Diatomite Achieve More Efficient Particle Retention than Filters Solely Containing Cellulose Fibers. Front Plant Sci 6, 1134. http://doi.org/10.3389/fpls.2015.01134

Buyel, J.F., Twyman, R.M., Fischer, R., 2015b. Extraction and downstream processing of plant-derived recombinant proteins. Biotechnol. Adv. 33, 902–913. http://doi.org/10.1016/j.biotechadv.2015.04.010

Cremer, C., Vierbuchen, T., Hein, L., Fischer, R., Barth, S., Nachreiner, T., 2015. Angiogenin mutants as novel effector molecules for the generation of fusion proteins with increased cytotoxic potential. J. Immunother. 38, 85–95. http://doi.org/10.1097/CJI.0000000000000053

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Epping, J., van Deenen, N., Niephaus, E., Stolze, A., Fricke, J., Huber, C., Eisenreich, W., Twyman, R.M., Prüfer, D., Schulze Gronover, C., 2015. A rubber transferase activator is necessary for natural rubber biosynthesis in dandelion. Nature Plants 1, 15048. http://doi.org/10.1038/nplants.2015.48

Faber, B.W., Hellwig, S., Houard, S., Havelange, N., Drossard, J., Mertens, H., Croon ,A., Byrne, R., van der Werff, N., van der Eijk, M., Thomas, A.W., Kocken, C.H.M. and Remarque, E.J.: Production, quality control, stability and pharmacotoxicity of a mixture of three cGMP-produced Diversity Covering Plasmodium falciparum AMA1 proteins, submitted

Fritsch, L., Fischer, R., Wambach, C., Dudek, M., Schillberg, S., Schröper, F., 2015. Next-generation sequencing is a robust strategy for the high-throughput detection of zygosity in transgenic maize. Transgenic Res. 24, 615–623. http://doi.org/10.1007/s11248-015-9864-x

Hristodorov, D., Mladenov, R., von Felbert, V., Huhn, M., Fischer, R., Barth, S., Thepen, T., 2015. Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies. MAbs 7, 853–862. http://doi.org/10.1080/19420862.2015.1066950

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Kapelski, S., Boes, A., Spiegel, H., de Almeida, M., Klockenbring, T., Reimann, A., Fischer, R., Barth, S., Fendel, R., 2015a. Fast track antibody V-gene rescue, recombinant expression in plants and characterization of a PfMSP4-specific antibody. Malar. J. 14, 50. http://doi.org/10.1186/s12936-015-0577-7

Kapelski, S., de Almeida, M., Fischer, R., Barth, S., Fendel, R., 2015b. Antimalarial activity of granzyme B and its targeted delivery by a granzyme B-single-chain Fv fusion protein. Antimicrob. Agents Chemother. 59, 669–672. http://doi.org/10.1128/AAC.04190-14

Laibach, N., Hillebrand, A., Twyman, R.M., Prüfer, D., Schulze Gronover, C., 2015. Identification of a Taraxacum brevicorniculatum rubber elongation factor protein that is localized on rubber particles and promotes rubber biosynthesis. Plant J. 82, 609–620. http://doi.org/10.1111/tpj.12836

Ma, J.K.-C., Drossard, J., Lewis, D., Altmann, F., Boyle, J., Christou, P., Cole, T., Dale, P., van Dolleweerd, C.J., Isitt, V., Katinger, D., Lobedan, M., Mertens, H., Paul, M.J., Rademacher, T., Sack, M., Hundleby, P.A.C., Stiegler, G., Stoger, E., Twyman, R.M., Vcelar, B., Fischer, R., 2015. Regulatory approval and a first-in-human phase I clinical trial of a monoclonal antibody produced in transgenic tobacco plants. Plant Biotechnol. J. 13, 1106–1120. http://doi.org/10.1111/pbi.12416

Maskus, D.J., Bethke, S., Seidel, M., Kapelski, S., Addai-Mensah, O., Boes, A., Edgü, G., Spiegel, H., Reimann, A., Fischer, R., Barth, S., Klockenbring, T., Fendel, R., 2015. Isolation, production and characterization of fully human monoclonal antibodies directed to Plasmodium falciparum MSP10. Malar. J. 14, 276. http://doi.org/10.1186/s12936-015-0797-x

Mladenov, R., Hristodorov, D., Cremer, C., Hein, L., Kreutzer, F., Stroisch, T., Niesen, J., Brehm, H., Blume, T., Brümmendorf, T.H., Jost, E., Thepen, T., Fischer, R., Stockmeyer, B., Barth, S., Stein, C., 2015. The Fc-alpha receptor is a new target antigen for immunotherapy of myeloid leukemia. Int. J. Cancer 137, 2729–2738. http://doi.org/10.1002/ijc.29628

Niesen, J., Brehm, H., Stein, C., Berges, N., Pardo, A., Fischer, R., Ten Haaf, A., Gattenlöhner, S., Tur, M.K., Barth, S., 2015a. In vitro effects and ex vivo binding of an EGFR-specific immunotoxin on rhabdomyosarcoma cells. J. Cancer Res. Clin. Oncol. 141, 1049–1061. http://doi.org/10.1007/s00432-014-1884-z

Niesen, J., Stein, C., Brehm, H., Hehmann-Titt, G., Fendel, R., Melmer, G., Fischer, R., Barth, S., 2015b. Novel EGFR-specific immunotoxins based on panitumumab and cetuximab show in vitro and ex vivo activity against different tumor entities. J. Cancer Res. Clin. Oncol. 141, 2079–2095. http://doi.org/10.1007/s00432-015-1975-5

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Popovic, M., Prodanovic, R., Ostafe, R., Schillberg, S., Fischer, R., Gavrovic-Jankulovic, M., 2015. Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens. Immunol. Res. 61, 230–239. http://doi.org/10.1007/s12026-014-8614-0

Raven, N., Rasche, S., Kuehn, C., Anderlei, T., Klöckner, W., Schuster, F., Henquet, M., Bosch, D., Büchs, J., Fischer, R., Schillberg, S., 2015. Scaled-up manufacturing of recombinant antibodies produced by plant cells in a 200-L orbitally-shaken disposable bioreactor. Biotechnol. Bioeng. 112, 308–321. http://doi.org/10.1002/bit.25352

Rettcher, S., Jungk, F., Kühn, C., Krause, H.-J., Nölke, G., Commandeur, U., Fischer, R., Schillberg, S., Schröper, F., 2015. A simple and portable magnetic immunoassay for the rapid detection and sensitive quantification of plant viruses. Appl. Environ. Microbiol. AEM.03667-14. http://doi.org/10.1128/AEM.03667-14

Sack, M., Hofbauer, A., Fischer, R., Stoger, E., 2015a. The increasing value of plant-made proteins. Curr. Opin. Biotechnol. 32, 163–170. http://doi.org/10.1016/j.copbio.2014.12.008

Sack, M., Rademacher, T., Spiegel, H., Boes, A., Hellwig, S., Drossard, J., Stoger, E., Fischer, R., 2015b. From gene to harvest: insights into upstream process development for the GMP production of a monoclonal antibody in transgenic tobacco plants. Plant Biotechnol. J. 13, 1094–1105. http://doi.org/10.1111/pbi.12438

Schubert, M., Houdelet, M., Kogel, K.-H., Fischer, R., Schillberg, S., Nölke, G., 2015. Thanatin confers partial resistance against aflatoxigenic fungi in maize (Zea mays). Transgenic Res. 24, 885–895. http://doi.org/10.1007/s11248-015-9888-2

Spiegel, H., Boes, A., Voepel, N., Beiss, V., Edgue, G., Rademacher, T., Sack, M., Schillberg, S., Reimann, A., Fischer, R., 2015. Application of a Scalable Plant Transient Gene Expression Platform for Malaria Vaccine Development. Front. Plant Sci. 1169. http://doi.org/10.3389/fpls.2015.01169

A - C

Brehm, H., Niesen, J., Mladenov, R., Stein, C., Pardo, A., Fey, G., Helfrich, W., Fischer, R., Gattenlöhner, S., Barth, S., 2014. A CSPG4-specific immunotoxin kills rhabdomyosarcoma cells and binds to primary tumor tissues. Cancer Lett. 352, 228–235. http://doi.org/10.1016/j.canlet.2014.07.006

Buntru, M., Vogel, S., Spiegel, H., Schillberg, S., 2014. Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system. BMC Biotechnology 14, 37. http://doi.org/10.1186/1472-6750-14-37

D - I

Havenith, H., Raven, N., Fiore, S.D., Fischer, R., Schillberg, S., 2014. Image-based analysis of cell-specific productivity for plant cell suspension cultures. Plant Cell Tiss Organ Cult 117, 393–399. http://doi.org/10.1007/s11240-014-0448-x

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Kern, S., Agarwal, S., Huber, K., Gehring, A.P., Strödke, B., Wirth, C.C., Brügl, T., Abodo, L.O., Dandekar, T., Doerig, C., Fischer, R., Tobin, A.B., Alam, M.M., Bracher, F., Pradel, G., 2014. Inhibition of the SR Protein-Phosphorylating CLK Kinases of Plasmodium falciparum Impairs Blood Stage Replication and Malaria Transmission. PLoS One 9. http://doi.org/10.1371/journal.pone.0105732

Mandal, M.K., Fischer, R., Schillberg, S., Schiermeyer, A., 2014. Inhibition of protease activity by antisense RNA improves recombinant protein production in Nicotiana tabacum cv. Bright Yellow 2 (BY-2) suspension cells. Biotechnol J 9, 1065–1073. http://doi.org/10.1002/biot.201300424

Müller, B., Groscurth, S., Menzel, M., Rüping, B.A., Twyman, R.M., Prüfer, D., Noll, G.A., 2014. Molecular and ultrastructural analysis of forisome subunits reveals the principles of forisome assembly. Ann. Bot. 113, 1121–1137. http://doi.org/10.1093/aob/mcu036

Nölke, G., Houdelet, M., Kreuzaler, F., Peterhänsel, C., Schillberg, S., 2014. The expression of a recombinant glycolate dehydrogenase polyprotein in potato (Solanum tuberosum) plastids strongly enhances photosynthesis and tuber yield. Plant Biotechnol J 12, 734–742. http://doi.org/10.1111/pbi.12178

Ostafe, R., Prodanovic, R., Nazor, J., Fischer, R., 2014. Ultra-High-Throughput Screening Method for the Directed Evolution of Glucose Oxidase. Chemistry & Biology 21, 414–421. http://doi.org/10.1016/j.chembiol.2014.01.010

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Schramm, O.G., López-Cortés, X., Santos, L.S., Laurie, V.F., Nilo, F.D.G., Krolik, M., Fischer, R., Fiore, S.D., 2013. pH-dependent nano-capturing of tartaric acid using dendrimers. Soft Matter 10, 600–608. http://doi.org/10.1039/C3SM52255E

Sommer, J., Garbers, C., Wolf, J., Trad, A., Moll, J.M., Sack, M., Fischer, R., Grötzinger, J., Waetzig, G.H., Floss, D.M., Scheller, J., 2014. Alternative intronic polyadenylation generates the interleukin-6 trans-signaling inhibitor sgp130-E10. J. Biol. Chem. 289, 22140–22150. http://doi.org/10.1074/jbc.M114.560938

Stoger, E., Fischer, R., Moloney, M., Ma, J.K.-C., 2014. Plant molecular pharming for the treatment of chronic and infectious diseases. Annu Rev Plant Biol 65, 743–768. http://doi.org/10.1146/annurev-arplant-050213-035850

V - Z

Vasilev, N., Schmitz, C., Grömping, U., Fischer, R., Schillberg, S., 2014. Assessment of cultivation factors that affect biomass and geraniol production in transgenic tobacco cell suspension cultures. PLoS ONE 9, e104620. http://doi.org/10.1371/journal.pone.0104620

Voepel, N., Boes, A., Edgue, G., Beiss, V., Kapelski, S., Reimann, A., Schillberg, S., Pradel, G., Fendel, R., Scheuermayer, M., Spiegel, H., Fischer, R., 2014. Malaria vaccine candidate antigen targeting the pre-erythrocytic stage of Plasmodium falciparum produced at high level in plants. Biotechnol J 9, 1435–1445. http://doi.org/10.1002/biot.201400350

Wirth, C.C., Glushakova, S., Scheuermayer, M., Repnik, U., Garg, S., Schaack, D., Kachman, M.M., Weißbach, T., Zimmerberg, J., Dandekar, T., Griffiths, G., Chitnis, C.E., Singh, S., Fischer, R., Pradel, G., 2014. Perforin-like protein PPLP2 permeabilizes the red blood cell membrane during egress of Plasmodium falciparum gametocytes. Cell. Microbiol. 16, 709–733. http://doi.org/10.1111/cmi.12288

Zielonka, S., Ernst, A.M., Hawat, S., Twyman, R.M., Prüfer, D., Noll, G.A., 2014. Characterization of five subgroups of the sieve element occlusion gene family in Glycine max reveals genes encoding non-forisome P-proteins, forisomes and forisome tails. Plant Mol. Biol. 86, 51–67. http://doi.org/10.1007/s11103-014-0211-z