Veröffentlichungen Molekulare Biotechnologie

Grundmann L., Känel A., Muth J., Beinecke F., Jekat M., Shen Y., Kudithipudi C., Xu D., Yang J., Warek U., Strickland J., Prüfer D., Noll G.A.
Tissue-specific expression of barnase in tobacco delays axillary shoot development after topping
(2021) Plant Biotechnology Journal DOI

Kronenberg, J., Schrödter, K., Noll, G.A., Twyman, R.M., Prüfer, D., Känel, P.
The tobacco phosphatidylethanolamine-binding protein NtFT4 simultaneously improves vitality, growth, and protein yield in human cells (2021) Biotechnology and Bioengineering, 118 (10), 3770-3786. DOI

Laibach N., Müller B., Pleissner D., Raber W., Smetana S.
An integrated, modular biorefinery for the treatment of food waste in urban areas
(2021) Case Studies in Chemical and Environmental Engineering, 4, DOI

Rose J., Brand I., Bilstein-Schloemer M., Jachimska B., Twyman R.M., Prüfer D., Noll G.A.
The Ca2+ response of a smart forisome protein is dependent on polymerization (2021) Protein Science, DOI

Rose, J., Müller, B., Groscurth, S., Giese, J., Eirich, J., Finkemeier, I., Twyman, R.M., Prüfer, D., Noll, G.A.
The functionality of plant mechanoproteins (forisomes) is dependent on the dual role of conserved cysteine residues (2021) International Journal of Biological Macromolecules DOI

Solís J.L., Muth J., Canales J., Lizana C., Prüfer D., Riegel R., Behn A.
Allelic diversity of three anthocyanin synthesis genes in accessions of native Solanum tuberosum L. ssp. tuberosum at the Potato Genebank of the Universidad Austral de Chile (2021) Genetic Resources and Crop Evolution, DOI

Vasilev, H., Šmejkal, K., Gronover, C.S., Choi, Y.H., Prüfer, D., Jankovská, D., Ionkova, I.
Flavonol glycosides from aerial parts of Astragalus thracicus Griseb (2021) Phytochemistry Letters, 41, 119-122. DOI

Wollenweber, T.E., van Deenen, N., Roelfs, K.-U., Prüfer, D., Gronover, C.S.
Microscopic and transcriptomic analysis of pollination processes in self-incompatible Taraxacum koksaghyz (2021) Plants, 10 (3), art. no. 555,1-17. DOI






Bröker, J. N., Müller, B., Prüfer, D., Schulze Gronover, C.
Combinatorial metabolic engineering in
Saccharomyces cerevisiae for the enhanced production of the FPP-derived sesquiterpene germacrene (2020) Bioengineering 7(4), 135.

Benninghaus, V.A., Van Deenen, N., Müller, B., Roelfs, K.-U., Lassowskat, I., Finkemeier, I., Prüfer, D., Gronover, C.S.
Comparative proteome and metabolome analyses of latex-exuding and non-exuding Taraxacum koksaghyz roots provide insights into laticifer biology (2020) Journal of Experimental Botany, 71 (4), 1278-1293.

Capell, T., Twyman, R.M., Armario-Najera, V., Ma, J.K.-C., Schillberg, S., Christou, P. Potential Applications of Plant Biotechnology against SARS-CoV-2(2020) Trends in Plant Science.

Derya, S.M., Spiegel, H., Hanisch, F.-G., Morozov, V., Schroten, H., Jennewein, S., Parschat, K.
Biotechnologically produced fucosylated oligosaccharides inhibit the binding of human noroviruses to their natural receptors (2020) Journal of Biotechnology, 318, pp. 31-38.

Edgü, G., Freund, L.J., Hartje, S., Tacke, E., Hofferbert, H.-R., Twyman, R.M., Noll, G.A., Muth, J., Prüfer, D.
Fast, precise, and reliable multiplex detection of potato viruses by loop-mediated isothermal amplification (2020) International Journal of Molecular Sciences, 21 (22), 8741: 1-19.

Ilić Đurđić, K., Ece, S., Ostafe, R., Vogel, S., Balaž, A.M., Schillberg, S., Fischer, R., Prodanović, R.
Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability (2020) Journal of Bioscience and Bioengineering, 129 (6),664-671. 10.1016/j.jbiosc.2019.12.009

Ilić Đurđić, K., Ece, S., Ostafe, R., Vogel, S., Schillberg, S., Fischer, R., Prodanović, R.
Improvement in oxidative stability of versatile peroxidase by flow cytometry-based high-throughput screening system (2020) Biochemical Engineering Journal, 157, 107555. 10.1016/j.bej.2020.107555

Ilić Đurđić, K., Ostafe, R., Đurđević Đelmaš, A., Popović, N., Schillberg, S., Fischer, R., Prodanović, R.
Saturation mutagenesis to improve the degradation of azo dyes by versatile peroxidase and application in form of VP-coated yeast cell walls (2020) Enzyme and Microbial Technology, 136, e109509.

Fischer, R., Buyel, J.F.
Molecular farming – The slope of enlightenment(2020) Biotechnology Advances, 40, art. no. 107519.

Gengenbach, B.B., Opdensteinen, P., Buyel, J.F.
Robot Cookies – Plant Cell Packs as an Automated High-Throughput Screening Platform Based on Transient Expression (2020) Frontiers in Bioengineering and Biotechnology, 8, art. no. 393.

Janke, C., Gaida, S., Jennewein S.
The production of isoprene from cellulose using recombinant Clostridium cellulolyticum strains expressing isoprene synthase (2020) MicrobiologyOpen

Kirchhoff, J., Schiermeyer, A., Schneider, K., Fischer, R., Ainley, W.M., Webb, S.R., Schinkel, H, Schillberg, S.
Gene expression variability between randomly and targeted transgene integration events in tobacco suspension cell lines (2020) Plant Biotechnol Rep

Pietschmann, J., Spiegel, H., Krause, H.-J., Schillberg, S., Schröper, F.
Sensitive Aflatoxin B1 Detection Using Nanoparticle-Based Competitive Magnetic Immunodetection (2020) Toxins, 12 (5).

Rose, J., Visser, F., Müller, B., Senft, M., Groscurth, S., Sicking, K.F., Twyman, R.M., Prüfer, D., Noll, G.A.
Identification and molecular analysis of interaction sites in the MtSEO-F1 protein involved in forisome assembly (2020) International Journal of Biological Macromolecules, 144, 603-614.

Schiermeyer, A.
Optimizing product quality in molecular farming (2020) Current Opinion in Biotechnology, 61, 15-20.

Schmidt, F., Zimmermann, M., Lichtenauer, S., Wiedmann, D., Grundmann, L., Muth, J, Twyman, R, Prüfer, D, Noll, G
The Major Floral Promoter NtFT5 in tobacco (Nicotiana tabacum) is a promising target for crop improvement (2020) Front. Plant Sci. 10:1666.

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Achtsnicht, S., Neuendorf, C., Faßbender, T., Nölke, G., Offenhäusser, A., Krause, H.-J., Schröper, F.
Sensitive and rapid detection of cholera toxin subunit B using magnetic frequency mixing detection (2019) PLoS ONE, 14 (7), e0219356.

Achtsnicht, S., Tödter, J., Niehues, J., Telöken, M., Offenhäusser, A., Krause, H.-J., Schröper, F.
3D printed modular immunofiltration columns for frequency mixing-based multiplex magnetic immunodetection (2019) Sensors (Switzerland), 19 (1), 148.

Arcalis, E., Ibl, V., Hilscher, J., Rademacher, T., Avesani, L., Morandini, F., Bortesi, L., Pezzotti, M., Vitale, A., Pum, D., De Meyer, T., Depicker, A., Stoger, E.
Russell-like bodies in plant seeds share common features with prolamin bodies and occur upon recombinant protein production (2019) Frontiers in Plant Science, 10, 777.

Buyel, J.F.
Hitze stabilisiert Malariaimpfstoffkandidaten in komplexen Proteinextrakten (2019) BioSpektrum, 25 (2), 163-166.

Buyel, J.F.
Plant molecular farming – Integration and exploitation of side streams to achieve sustainable biomanufacturing (2019) Frontiers in Plant Science, 9, 1893.

Conrady, M., Lemoine, A., Limberg, M.H., Oldiges, M., Neubauer, P., Junne, S.
Carboxylic acid consumption and production by Corynebacterium glutamicum (2019) Biotechnology Progress, e2804.


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Gengenbach, B.B., Keil, L.L., Opdensteinen, P., Müschen, C.R., Melmer, G., Lentzen, H., Bührmann, J., Buyel, J.F.
Comparison of microbial and transient expression (tobacco plants and plant-cell packs) for the production and purification of the anticancer mistletoe lectin viscumin (2019) Biotechnology and Bioengineering, 116 (9), 2236-2249.

Hussain, A.F., Heppenstall, P.A., Kampmeier, F., Meinhold-Heerlein, I., Barth, S.
One-step site-specific antibody fragment auto-conjugation using SNAP-tag technology (2019) Nature Protocols, 14, 3101-3125.

Jansing, J., Buyel, J.F.
The Correlation Between DsRed mRNA Levels and Transient DsRed Protein Expression in Plants Depends on Leaf Age and the 5' Untranslated Region (2019) Biotechnology Journal 14 (3), 1800075.

Jansing, J., Schiermeyer, A., Schillberg, S., Fischer, R., Bortesi, L.
Genome editing in agriculture: Technical and practical considerations (2019) International Journal of Molecular Sciences, 20 (12), 2888.

Kern, K., Havenith, H., Delaroque, N., Rautenberger, P., Lehmann, J., Fischer, M., Spiegel, H., Schillberg, S., Ehrentreich-Foerster, E., Aurich, S., Treudler, R., Szardenings, M.
The immunome of soy bean allergy: Comprehensive identifi­cation and characterization of epitopes (2019) Clinical and Experi­mental Allergy, 49 (2), 239-251.

Knödler, M., Rühl, C., Emonts, J., Buyel, J. F.
Seasonal weather changes affect the yield and quality of recombinant proteins produced in transgenic tobacco plants in a greenhouse setting (2019) Frontiers in Plant Science, 10, 1245.

Knödler, M., Rühl C., Opdensteinen, P., Buyel, J.F.
Activated cross-linked agarose for the rapid development of affinity chromatography resins – Antibody capture as a case study (2019) Journal of Visualized Experiments, 150, e59933.

Kovačević, G., Ostafe, R., Fischer, R., Prodanović, R.
Influence of methionine residue position on oxidative stability of glucose oxidase from Aspergillus niger (2019) Biochemical Engineering Journal, 146, 143-149.

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Maennling, A.E., Tur, M.K., Niebert, M., Klockenbring, T., Zeppernick, F., Gattenlöhner, S., Meinhold-Heerlein, I., Hussain, A.F.
Molecular targeting therapy against egfr family in breast cancer: Progress and future potentials (2019) Cancers, 11 (12), e1826.

Niephaus, E., Müller, B., van Deenen, N., Lassowskat, I., Bonin, M., Finkemeier, I., Prüfer, D., Schulze Gronover, C.
Uncovering mechanisms of rubber biosynthesis in Taraxacum koksaghyz - role of cis-prenyltransferase-like 1 protein (2019) The Plant Journal.

Nölke, G., Barsoum, M., Houdelet, M., Arcalis, E., Kreuzaler, F., Fischer, R., Schillberg, S.
The integration of algal carbon concentration mechanism components into tobacco chloroplasts increases photosynthetic efficiency and biomass (2019). Biotechnology Journal, 14 (3), 1800170.

Opdensteinen, P., Clodt, J.I., Müschen, C.R., Filiz, V., Buyel, J.F.
A combined ultrafiltration/diafiltration step facilitates the purification of cyanovirin-n from transgenic tobacco extracts (2019) Frontiers in Bioengineering and Biotechnology, 6, 206.

Philipps, G., De Vries, S., Jennewein, S.
Development of a metabolic pathway transfer and genomic integration system for the syngas-fermenting bacterium Clostridium ljungdahlii (2019) Biotechnology for Biofuels, 12 (1), 112.

Pütter, K.M., van Deenen, N., Müller, B., Fuchs, L., Vorwerk, K., Unland, K., Bröker, J.N., Scherer, E., Huber, C., Eisenreich, W., Prüfer, D., Schulze Gronover, C.
The enzymes OSC1 and CYP716A263 produce a high variety of triterpenoids in the latex of Taraxacum koksaghyz (2019) Scientific Reports, 9 (1), 5942.

Rademacher, T., Sack, M., Blessing, D., Fischer, R., Holland, T., Buyel, J.
Plant cell packs: A scalable platform for recombinant protein production and metabolic engineering (2019) Plant Biotechnology Journal, 17 (8), 1560-1566.

Rasche S., Schillberg S., Derwenskus F., Schmid-Staiger U., Schließmann U.
Organismen als Produzenten (2019) In Biologische Transformation, Springer Vieweg, 183-202.

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Santos, R.B., Pires, A.S., van der Hoorn, R.A.L., Schiermeyer, A., Abranches, R.
Generation of transgenic cell suspension cultures of the model legume Medicago truncatula: a rapid method for Agrobacterium mediated gene transfer (2019) Plant Cell, Tissue and Organ Culture, 136 (3), 445-450.

Schiermeyer, A., Schneider, K, Kirchhoff, J., Schmelter, T., Koch, N, Ke, J., Herwartz, D., Blue, R., Marri, P., Samuel, P., Corbin, D.R., Webb, S.R., Gonzalez, D.O., Folkerts, O., Fischer, R., Schinkel, H., Ainley, W. M., Schillberg, S.
Targeted insertion of large DNA sequences by homology‐directed repair or non‐homologous end joining in engineered tobacco BY‐2 cells using designed zinc finger nucleases (2019) Plant direct 3 , No.7, Art. e00153.

Schillberg, S., Raven, N., Spiegel, H., Rasche, S., Buntru, M.
Critical analysis of the commercial potential of plants for the production of recombinant proteins (2019) Frontiers in Plant Science, 10, 720.

Schmitz, C., Auza, L.G., Koberidze, D., Rasche, S., Fischer, R., Bortesi, L.
Conversion of chitin to defined chitosan oligomers: Current status and future prospects (2019) Marine Drugs, 17 (8), 452.

Schubert, M., Xue, S., Ebel, F., Vaggelas, A., Krylov, V.B., Nifantiev, N.E., Chudobová, I., Schillberg, S., Nölke, G.
Monoclonal antibody AP3 binds galactomannan antigens displayed by the pathogens Aspergillus flavus, A. fumigatus, and A. parasiticus (2019) Frontiers in Cellular and Infection Microbiology, 9, 234.

Spiegel, H., Boes, A., Perales Morales, C., Rademacher, T., Buyel, J.F.
Ready to use stocks of Agrobacterium tumefaciens can simplify process development for the production of recombinant proteins by transient expression in plants (2019) Biotechnology Journal, 1900113.

Ueberham, E., Spiegel, H., Havenith, H., Rautenberger, P., Lidzba, N., Schillberg, S., Lehmann, J.
Simplified tracking of a soy allergen in processed food using a monoclonal antibody-based sandwich ELISA targeting the soybean 2S Albumin Gly m 8 (2019) Journal of Agricultural and Food Chemistry, 67, 8660-8667.

van Deenen, N., Unland, K., Prüfer, D., Schulze Gronover, C.
Oxidosqualene cyclase knock-down in latex of Taraxacum koksaghyz reduces triterpenes in roots and separated natural rubber (2019) Molecules, 24 (15), 2703.

Volke, D.C., Rohwer, J., Fischer, R., Jennewein, S.
Investigation of the methylerythritol 4-phosphate pathway for microbial terpenoid production through metabolic control analysis (2019) Microbial Cell Factories, 18, 192.

Wieghaus, A., Prüfer, D., Schulze Gronover, C.
Loss of function mutation of the Rapid Alkalinization Factor (RALF1)-like peptide in the dandelion Taraxacum koksaghyz entails a high biomass taproot phenotype (2019) PLoS ONE, 14 (5), e0217454.

Wu, D., von Roepenack-Lahaye, E., Buntru, M., de Lange, O., Schandry, N., Pérez-Quintero, A.L., Weinberg, Z., Lowe-Power, T.M., Szurek, B., Michael, A.J., Allen, C., Schillberg, S., Lahaye, T.
A pathogen effector subverts translational regulation to boost host polyamine levels (2019) Social Science Research Network.

Zafarnia, S., Mrugalla, A., Rix, A., Doleschel, D., Gremse, F., Wolf, S.D., Buyel, J.F., Albrecht, U., Bode, J.G., Kiessling, F., Lederle, W.
Non-invasive imaging and modeling of liver regeneration after partial hepatectomy (2019) Frontiers in Physiology, 10, 904.

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Beinecke, F.A., Grundmann, L., Wiedmann, D.R., Schmidt, F.J., Caesar, A.S., Zimmermann, M., Lahme, M., Twyman, R.M., Prüfer, D., Noll, G.A.
The FT/FD-dependent initiation of flowering under long-day conditions in the day-neutral species Nicotiana tabacum originates from the facultative short-day ancestor ­Nicotiana ­tomentosiformis (2018) Plant Journal, 96 (2), 329-342.

Bröker, J.N., Laibach, N., Müller, B., Prüfer, D., Schulze Gronover, C.
Taraxacum brevicorniculatum
rubber elongation factor TbREF associates with lipid droplets and affects lipid turn-over in yeast (2018) Biotechnology Reports, 20, e00290.

Bröker, J.N., Müller, B., van Deenen, N., Prüfer, D., Schulze Gronover, C.
Upregulating the mevalonate pathway and repressing sterol synthesis in Saccharomyces cerevisiae enhances the production of triterpenes (2018) Applied Microbiology and Biotechnology 1-12

Buyel, J.F.
Plants as sources of natural and recombinant anti-cancer agents (2018) Biotechnology Advances, 36 (2), 506-520.

Chouman, K., Korioth-Schmitz, B., Sack, M., Schmitz, J.E., Pham, A.T., Fischer, R., Barth, S., Klockenbring, T., Fendel, R.
Characterization of new anti-IL-6 antibodies revealed high potency candidates for intracellular cytokine detection and specific targeting of IL-6 receptor binding sites (2018) European Cytokine Network, 29 (2), 59-72.

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Gengenbach, B.B., Müschen, C.R., Buyel J.F.
Expression and purification of human phosphatase and actin regulator 1 (PHACTR1) in plant-based systems (2018) Protein Expression and Purification, 151, 46-55 [Epub ahead of print]

Gresch, G., Schenke, L., Mladenov, R., Zwirner, S., Cremer, C., Niesen, J., Grieger, E., Brümmendorf, T., Jost, E., Fischer, R., Stockmeyer, B., Barth, S., Nachreiner, T., Stein, C.
Elimination of different leukaemia subtypes using novel CD89-specific human cytolytic fusion proteins (2018) British ­Journal of Haematology, 183 (2), 313-317.

Hausmann, R., Chudobová, I., Spiegel, H., Schillberg, S.
Proteomic analysis of CHO cell lines producing high and low quantities of a recombinant antibody before and after selection with methotrexate (2018) Journal of Biotechnology, 265, 65-69.

Holland, T., Buyel, J.F. Bioreactor-based production of glycoproteins in plant cell suspension cultures (2018) Methods in Molecular Biology, 1674, 129-146.

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Jansing, J., Buyel, J.F.
The Correlation Between DsRed mRNA Levels and Transient DsRed Protein Expression in Plants Depends on Leaf Age and the 5' Untranslated Region (2018) Biotechnology Journal [Epub ahead of print]

Kern, K., Havenith, H., Delaroque, N., Rautenberger, P., Lehmann, J., Fischer, M., Spiegel, H., Schillberg, S., Ehrentreich-Foerster, E., Aurich, S., Treudler, R., Szardenings, M.
The immunome of soy bean allergy: Comprehensive identifi­cation and characterization of epitopes (2018) Clinical and Experi­mental Allergy, e13285.

Laibach, N., Schmidl, S., Müller, B., Bergmann, M., Prüfer, D., Schulze Gronover, C.
Small rubber particle proteins from Taraxacum brevicorniculatum promote stress tolerance and influence the size and distribution of lipid droplets and artificial poly(cis-1,4-isoprene) bodies (2018) Plant Journal, 93 (6), 1045-1061.

Lim, S.S.Y., Chua, K.H., Nölke, G., Spiegel, H., Goh, W.L., Chow, S.C., Kee, B.P., Fischer, R., Schillberg, S., Othman, R.Y.
Plant-derived chimeric antibodies inhibit the invasion of human fibroblasts by Toxoplasma gondii  (2018) PeerJ, 2018 (12), 5780.

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Martínez, M.E., Poirrier, P., Prüfer, D., Schulze, C., Jorquera, L., Ferrer, P., Díaz, K., Chamy, R.
Kinetics and modeling of cell growth for potential anthocyanin induction in cultures of Taraxacum officinale G.H. Weber ex Wiggers (Dandelion) in vitro (2018) Electronic Journal of Biotechnology, 36, 15-23.

McElroy, C., & Jennewein, S. (2018). Taxol® biosynthesis and production: from forests to fermenters. In Biotechnology of Natural Products (pp. 145-185). Springer, Cham.

Menzel, S., Holland, T., Boes, A., Spiegel, H., Fischer, R., Buyel, J.F.
Downstream processing of a plant-derived malaria transmission-blocking vaccine candidate (2018) Protein Expression and Purification, 152, 122-130.

Nölke, G., Barsoum, M., Houdelet, M., Arcalis, E., Kreuzaler, F., Fischer, R., Schillberg, S.
The integration of algal carbon concentration mechanism components into tobacco chloroplasts increases photosynthetic efficiency and biomass (2018). Biotechnology Journal, 1800170.

Rühl, C., Knödler, M., Opdensteinen, P., Buyel, J.F.
A linear epitope coupled to DsRed provides an affinity ligand for the capture of monoclonal antibodies (2018) Journal of Chromatography A, 1571, 55-64.

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Santos, R.B., Chandrasekar, B., Mandal, M.K., Kaschani, F., Kaiser, M., Both, L., van der Hoorn, R.A.L., Schiermeyer, A., Abranches, R.
Low Protease Content in Medicago truncatula Cell Cultures Facilitates Recombinant Protein Production (2018) Biotechnology Journal, 13 (7), 1800050.

Schubert, M., Spiegel, H., Schillberg, S., Nölke, G. Aspergillus-specific antibodies – Targets and applications (2018) Biotechnology Advances, 36 (4), 1167-1184.

Spiegel, H., Stöger, E., Twyman, R.M., Buyel, J.F.
Current status and perspectives of the molecular farming landscape (2018) Molecular Pharming: Applications, Challenges and Emerging Areas, 1-23.

Tyutereva, E.V., Dobryakova, K.S., Schiermeyer, A., Shishova, M.F., Pawlowski, K., Demidchik, V., Reumann, S., Voitsekhovskaja, O.V. The levels of peroxisomal catalase protein and activity modulate the onset of cell death in tobacco BY-2 cells via reactive oxygen species levels and autophagy (2018) Functional Plant Biology, 45 (1-2), 247-258.

Wollschlaeger, C., Meinhold-Heerlein, I., Cong, X., Bräutigam, K., Di Fiore, S., Zeppernick, F., Klockenbring, T., Stickeler, E., Barth, S., Hussain, A.F.
Simultaneous and Independent Dual Site-Specific Self-Labeling of Recombinant Antibodies (2018) Bioconjugate Chemistry, 29 (11), 3586-3594.

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Akinrinmade, O.A., Jordaan, S., Hristodorov, D., Mladenov, R., Mungra, N., Chetty, S., Barth, S., 2017. Human MAP tau based targeted cytolytic fusion proteins. Biomedicines No. 5(3).

Augustine, S.M., 2017. CRISPR-cas9 system as a genome editing tool in sugarcane in: Mohan C. (Ed.) Sugarcane Biotechnology: Challenges and Prospects Springer International Publishing pp. 155-172.

Bauerschlag, D., Meinhold-Heerlein, I., Maass, N., Bleilevens, A., Bräutigam, K., Al Rawashdeh, W., Di Fiore, S., Haugg, A.M., Gremse, F., Steitz, J., Fischer, R., Stickeler, E., Barth, S., Hussain, A.F., 2017. Detection and Specific Elimination of EGFR+ Ovarian Cancer Cells Using a Near Infrared Photoimmunotheranostic Approach. Pharmaceutical Research 34, 696–703.

Berges, N., Arens, K., Kreusch, V., Fischer, R., Di Fiore, S., 2017. Toward discovery of novel microtubule targeting agents: A SNAP-tag-based high-content screening assay for the analysis of microtubule dynamics and cell cycle progression. SALAS Discovery 22, 387–398.

Braig, F., Kriegs, M., Voigtlaender, M., Habel, B., Grob, T., Biskup, K., Blanchard, V., Sack, M., Thalhammer, A., Batalla, I.B., Braren, I., Laban, S., Danielczyk, A., Goletz, S., Jakubowicz, E., Märkl, B., Trepel, M., Knecht, R., Riecken, K., Fehse, B., Loges, S., Bokemeyer, C., Binder, M., 2017. Cetuximab resistance in head and neck cancer is mediated by EGFR-K521 polymorphism. Cancer Research 77, 1188–1199.

Buyel, J.F., 2017a. Blanching facilitates the purification of recombinant proteins from plants. BioSpektrum 23, 522–524.

Buyel, J.F., 2017b. How plants can contribute to the supply of anticancer compounds, in: Biotechnology and Production of Anti-Cancer Compounds. pp. 39–72.

Buyel, J.F., Twyman, R.M., Fischer, R., 2017. Very-large-scale production of antibodies in plants: The biologization of manufacturing. Biotechnology Advances 35, 458–465.

Choudhary, S., Barth, S., Verma, R.S., 2017. SNAP-Tag Technology: A Promising Tool for Ex Vivo Immunophenotyping. Molecular Diagnosis and Therapy 21, 315–326.

Chouman, K., Woitok, M., Mladenov, R., Kessler, C., Weinhold, E., Hanz, G., Fischer, R., Meinhold-Heerlein, I., Bleilevens, A., Gresch, G., Haugg, A.M., Zeppernick, F., Bauerschlag, D., Maass, N., Stickeler, E., Kolberg, K., Hussain, A.F., 2017. Fine tuning antibody conjugation methods using SNAP-tag technology. Anti-Cancer Agents in Medicinal Chemistry 17, 1434–1440.

Costa, M.P., Feitosa, A.C.S., Oliveira, F.C.E., Cavalcanti, B.C., Dias, G.G., Caetano, E.W.S., Sales, F.A.M., Freire, V.N., Di Fiore, S., Fischer, R., Ladeira, L.O., Da Silva Júnior, E.N., Pessoa, C., 2017. Encapsulation of nor-β-lapachone into poly(d,l)-lactide-: Co -glycolide (PLGA) microcapsules: Full characterization, computational details and cytotoxic activity against human cancer cell lines. MedChemComm 8, 1993–2002.

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Ece, S., Lambertz, C., Fischer, R., Commandeur, U., 2017. Heterologous expression of a Streptomyces cyaneus laccase for biomass modification applications. AMB Express 7.

Edgue, G., Twyman, R.M., Beiss, V., Fischer, R., Sack, M., 2017. Antibodies from plants for bionanomaterials. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology 9.

Fischer, R., Schillberg, S., 2017. Molecular Farming, in: Thomas, B., Murray, B.G., Murphy, D.J. (Eds.), Encyclopedia of Applied Plant Sciences (Second Edition). Academic Press, Oxford, pp. 77–82.

Gresch, G., Schenke, L., Mladenov, R., Zwirner, S., Cremer, C., Niesen, J., Grieger, E., Brümmendorf, T., Jost, E., Fischer, R., Stockmeyer, B., Barth, S., Nachreiner, T., Stein, C., 2017. Elimination of different leukaemia subtypes using novel CD89-specific human cytolytic fusion proteins. Br J Haematol n/a-n/a.

Grieger, E., Gresch, G., Niesen, J., Woitok, M., Barth, S., Fischer, R., Fendel, R., Stein, C., 2017. Efficient targeting of CD13 on cancer cells by the immunotoxin scFv13–ETA′ and the bispecific scFv [13xds16]. Journal of Cancer Research and Clinical Oncology 143, 2159–2170.

Havenith, H., Kern, K., Rautenberger, P., Spiegel, H., Szardenings, M., Ueberham, E., Lehmann, J., Buntru, M., Vogel, S., Treudler, R., Fischer, R., Schillberg, S., 2017. Combination of two epitope identification techniques enables the rational design of soy allergen Gly m 4 mutants. Biotechnology Journal 12.

Houdelet, M., Galinski, A., Holland, T., Wenzel, K., Schillberg, S., Buyel, J.F., 2017. Animal component-free Agrobacterium tumefaciens cultivation media for better GMP-compliance increases biomass yield and pharmaceutical protein expression in Nicotiana benthamiana. Biotechnology Journal 12.

Huck, N.V., Leissing, F., Majovsky, P., Buntru, M., Aretz, C., Flecken, M., Müller, J.P.J., Vogel, S., Schillberg, S., Hoehenwarter, W., Conrath, U., Beckers, G.J.M., 2017. Combined 15N-labeling and tandemmoac quantifies phosphorylation of map kinase substrates downstream of MKK7 in Arabidopsis. Frontiers in Plant Science No. 8.

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Kastilan, R., Boes, A., Spiegel, H., Voepel, N., Chudobová, I., Hellwig, S., Buyel, J.F., Reimann, A., Fischer, R., 2017. Improvement of a fermentation process for the production of two PfAMA1-DiCo-based malaria vaccine candidates in Pichia pastoris. Scientific Reports 7.

Kessler, C., Pardo, A., Tur, M.K., Gattenlöhner, S., Fischer, R., Kolberg, K., Barth, S., 2017. Novel PSCA targeting scFv-fusion proteins for diagnosis and immunotherapy of prostate cancer. Journal of Cancer Research and Clinical Oncology 143, 2025–2038.

Klose, D., Woitok, M., Niesen, J., Beerli, R.R., Grawunder, U., Fischer, R., Barth, S., Fendel, R., Nachreiner, T.:
Generation of an artificial human B cell line test system using Transpo-mAbTM technology to evaluate the therapeutic efficacy of novel antigen-specific fusion proteins. PLoS ONE (2017) No. 12(7)

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Purcell, O., Opdensteinen, P., Chen, W., Lowenhaupt, K., Brown, A., Hermann, M., Cao, J., Tenhaef, N., Kallweit, E., Kastilan, R., Sinskey, A.J., Perez-Pinera, P., Buyel, J.F., Lu, T.K., 2017. Production of Functional Anti-Ebola Antibodies in Pichia pastoris. ACS Synthetic Biology No. 6(12), 2183-2190.

Pütter, K.M., van Deenen, N., Unland, K., Prüfer, D., Schulze Gronover, C., 2017. Isoprenoid biosynthesis in dandelion latex is enhanced by the overexpression of three key enzymes involved in the mevalonate pathway. BMC Plant Biology 17.

Schillberg, S., Raven, N., Fischer, R., Twyman, R.M., Schiermeyer, A., 2017. Contained Molecular Farming Using Plant Cell and Tissue Cultures, in: Yoshida, T. (Ed.), Applied Bioengineering. Wiley-VCH Verlag GmbH & Co. KGaA, pp. 259–281.

Spiegel, H., Boes, A., Fendel, R., Reimann, A., Schillberg, S., Fischer, R., 2017. Immunization with the malaria diversity-covering blood-stage vaccine candidate plasmodium falciparum apical membrane antigen 1 DiCo in complex with its natural ligand PfRon2 does not improve the in vitro efficacy. Frontiers in Immunology 8.

Stolze, A., Wanke, A., van Deenen, N., Geyer, R., Prüfer, D., Schulze Gronover, C., 2017. Development of rubber-enriched dandelion varieties by metabolic engineering of the inulin pathway. Plant Biotechnology Journal 15, 740–753.

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Woitok, M., Klose, D., Di Fiore, S., Richter, W., Stein, C., Gresch, G., Grieger, E., Barth, S., Fischer, R., Kolberg, K., Niesen, J., 2017. Comparison of a mouse and a novel human scFv-SNAP-auristatin F drug conjugate with potent activity against EGFR-overexpressing human solid tumor cells. OncoTargets and Therapy 10, 3313–3327.

Zhu, C., Bortesi, L., Baysal, C., Twyman, R.M., Fischer, R., Capell, T., Schillberg, S., Christou, P., 2017. Characteristics of Genome Editing Mutations in Cereal Crops. Trends in Plant Science 22, 38–52.

Zischewski, J., Fischer, R., Bortesi, L., 2017. Detection of on-target and off-target mutations generated by CRISPR/Cas9 and other sequence-specific nucleases. Biotechnology Advances 35, 95–104.


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Addai-Mensah, O., Seidel, M., Amidu, N., Maskus, D.J., Kapelski, S., Breuer, G., Franken, C., Owusu-Dabo, E., Frempong, M., Rakotozandrindrainy, R., Schinkel, H., Reimann, A., Klockenbring, T., Barth, S., Fischer, R., Fendel, R.: Acquired immune responses to three malaria vaccine candidates and their relationship to invasion inhibition in two populations naturally exposed to malaria. Malaria Journal. Online Journal (2016) No. 15: Art.65 ( )

Amoury, M., Bauerschlag, D., Zeppernick, F., Felbert, V., Berges, N., Di Fiore, S.D., Mintert, I., Bleilevens, A., Maass, N., Bräutigam, K., Meinhold-Heerlein, I., Stickeler, E., Barth, S., Fischer, R., Hussain, A.F.: Photoimmunotheranostic agents for triple-negative breast cancer diagnosis and therapy that can be activated on demand. OncoTarget 7 (2016), No. 34: 54925-54936 (

Amoury, M., Kolberg, K., Pham, A.-T., Hristodorov, D., Mladenov, R., Di Fiore, S., Helfrich, W., Kiessling, F., Fischer, R., Pardo, A., Thepen, T., Hussain, A.F., Nachreiner, T., Barth, S.: Granzyme B-based cytolytic fusion protein targeting EpCAM specifically kills triple negative breast cancer cells in vitro and inhibits tumor growth in a subcutaneous mouse tumor model. Cancer letters 372 (2016) No. 2: 201-209 (

Amoury, M., Mladenov, R., Nachreiner, T., Pham, A.-T., Hristodorov, D., Di Fiore, S., Helfrich, W., Pardo, A., Fey, G., Schwenkert, M., Thepen, T., Kiessling, F., Hussain, A.F., Fischer, R., Kolberg, K., Barth, S.: A novel approach for targeted elimination of CSPG4-positive triple-negative breast cancer cells using a MAP tau-based fusion protein. International Journal of Cancer 139 (2016) No. 4: 916-927 (

Arfi, Z.A., Hellwig, S., Drossard, J., Fischer, R., Buyel, J.F.: Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisCO depletion and the removal of endotoxins. Biotechnology Journal 11 (2016) No. 4: 507-518 (

Baysal, C., Bortesi, L., Zhu, C., Farré, G., Schillberg, S., Christou, P.: CRISPR/Cas9 activity in the rice OsBEIIb gene does not induce off-target effects in the closely related paralog OsBEIIa. Molecular Breeding 36 (2016) No. 8 (

Bialon, M., Grezella, C., Friesen, L., Sieben, T., Pham, A.T., Fischer, R., et al.: A monoclonal antibody that discriminates between SNAP-Tagged and CLIP-tagged proteins. Monoclonal antibodies in immunodiagnosis and immunotherapy. 2016 No. 35(3): 141-147 (

Bodewein, L., Schmelter, F., Di Fiore, S., Hollert, H., Fischer, R., Fenske, M.: Differences in toxicity of anionic and cationic PAMAM and PPI dendrimers in zebrafish embryos and cancer cell lines. Toxicology and Applied Pharmacology (2016) No. 395: 83-92 (

Boes, A., Reimann, A., Twyman, R.M., Fischer, R., Schillberg, S., Spiegel, H.: A plant-based transient expression system for the rapid production of malaria vaccine candidates. Methods in Molecular Biology (2016) No. 1404: 597-619 (

Boes, A., Spiegel, H., Kastilan, R., Bethke, S., Voepel, N., Chudobova, I., Bolscher, J.M., Dechering, K.J., Fendel, R., Buyel, J.F., Reimann, A., Schillberg, S., Fischer, R.: Analysis of the dose-dependent stage-specific in vitro efficacy of a multi-stage malaria vaccine candidate cocktail. Malaria Journal. Online Journal 15 (2016) Art. 279 (

Boesch, A.W., Osei-Owusu, N.Y., Crowley, A.R., Chu, T.H., Chan, Y.N., Weiner, J.A., Bharadwaj, P., Hards, R., Adamo, M.E., Gerber, S.A., Cocklin, S.L., Schmitz, J.E., Miles, A.R., Eckman, J.W., Belli, A.J., Reimann, K.A., Ackerman, M.E.: Biophysical and functional characterization of rhesus macaque IgG subclasses.  Front Immunology (2016) (

Bortesi, L., Zhu, C., Zischewski, J., Perez, L., Bassié, L., Nadi, R., Forni, G., Lade, S.B., Soto, E., Jin, X., Medina, V., Villorbina, G., Munoz, P., Farré, G., Fischer, R., Twyman, R.M., Capell, T., Christou, P., Schillberg, S.: Patterns of CRISPR/Cas9 activity in plants, animals and microbes. Plant biotechnology journal 14 (2016) No.12: 2203-2216 (

Buyel, J.F.: Numeric simulation can be used to predict heat transfer during the blanching of leaves and intact plants. Biochemical Engineering Journal (2016) No. 109: 118-126 (

Buyel, J.F.: Procedure to evaluate the efficiency of flocculants for the removal of dispersed particles from plant extracts. Journal of Visualized Experiments: JoVE. Online resource (2016) No.110: Art. e53940 (

Buyel, J.F., Gruchow, H.M., Tödter, N., Wehner, M.: Determination of the thermal properties of leaves by non-invasive contact free laser probing. Journal of Biotechnology (2016) No. 217: 100-108 (

Buyel, J.F., Hubbuch, J., Fischer, R.: Comparison of tobacco host cell protein removal methods by blanching intact plants or by heat treatment of extracts. Journal of Visualized Experiments: JoVE. Online resource (2016) No.114 (

Cartwright, E.K., Spicer, L., Smith, S.A., Lee, D., Fast, R., Paganini, S., Lawson, B.O., Nega, M., Easley, K., Schmitz, J.E., Bosinger, S.E., Paiardini, M., Chahroudi, A., Vanderford, T.H., Estes, J.D., Lifson, J.D., Derdeyn, C.A., Silvestri, G.: CD8+ lymphocytes are required for maintaining viral suppression in SIV-infected macaques treated with short-term antiretroviral therapy. Immunity. (2016) No. 45(3): 656-668 ( PMID: 27653601)

Carvajal-Rondanelli, P., Aróstica, M., Marshall, S.H., Albericio, F., Álvarez, C.A., Ojeda, C., Aguilar, L.F., Guzmán, F.: Inhibitory effect of short cationic homopeptides against Gram-negative bacteria. Amino Acids (2016) No. 48(6): 1445-1456 (

Castro, R.I., Forero-Doria, O., Guzmán, L., Laurie, V.F., Valdés, O., Ávila-Salas, F., López-Cortés, X., Santos, L.S.: New polymer for removal of wine phenolics: Poly(N-(3-(N-isobutyrylisobutyramido)-3-oxopropyl)acrylamide) (P-NIOA). Food Chemistry (2016) No. 213:  554-560 (

Chan, Y.N., Boesch, A.W., Osei-Owusu, N.Y., Emileh, A., Crowley, A.R., Cocklin, S.L., Finstad, S.L., Linde, C.H., Howell, R.A., Zentner, I., Cocklin, S., Miles, A.R., Eckman, J.W., Alter, G., Schmitz, J.E., Ackerman, M.E.: IgG binding characteristics of rhesus macaque FcgR. Journal of Immunology (2016) No. 197(7): 2936-2947 ( PMID: 27559046)

Choudhary, S., Pardo, A., Rosinke, R., Batra, J.K., Barth, S. Verma, R.S.: Targeting c-kit receptor in neuroblastomas and colorectal cancers using stem cell factor (SCF)-based recombinant bacterial toxins. Applied Microbiology and Biotechnology (2016) No. 100(1): 263-277 (

Cong, X., Cremer, C., Nachreiner, T., Barth, S., Carloni, P.: Engineered human angiogenin mutations in the placental ribonuclease inhibitor complex for anticancer therapy: Insights from enhanced sampling simulations. Protein Science : a publication of the Protein Society (2016) No. 25(8): 1451-1460 (

Costa, M.P., Feitosa, A.C.S., Oliveira, F.C.E., Cavalcanti, B.C., Da Silva, E.N., Jr., Dias, G.G., Sales, F.A.M., Sousa, B.L., Barroso-Neto, I.L., Pessoa, C., Caetano, E.W.S., Di Fiore, S., Fischer, R., Ladeira, L.O., Freire, V.N.: Controlled release of Nor-b-lapachone by PLGA Microparticles: a strategy for improving cytotoxicity against prostate cancer cells. Molecules 21 (2016) No. 7: 12 S. (


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Damm, T., Spiegel, H., Barth, S., Fischer, R., Naehring, J.: Development of a competitive cystatin C-Specific bioassay suitable for repetitivem measurements. PloS one. (2016) No. 11(1): e0147177 (

Fischer, R., Schulze Gronover, C., Prüfer, D: Naturkautschuk aus russischem Löwenzahn. Ressourceneffizienz, Schlüsseltechnologie für Wirtschaft&Gesellschaft. Fraunhofer Forschungsfokus, Hrsg. Reimund Neugebauer (2016) 232-329  

Fritsch, L., Soeur, R., Hansen, C., Fischer, R., Schillberg, S., Schröper, F.: Next-generation sequencing of amplicons is a rapid and reliable method for the detection of polymorphisms relevant for barley breeding. Molecular Breeding 36 (2016) No.83 (

Gaida, S.M., Liedtke, A., Jentges, A.H.W., Engels, B., Jennewein, S.: Metabolic engineering of clostridium cellulolyticum for the production of n-butanol from crystalline cellulose. Microbial Cell Factories. Online journal 15 (2016), Art.6 (

Hristodorov, D., Mladenov, R., Fischer, R., Barth, S., Thepen, T.: Fully human MAP-fusion protein selectively targets and eliminates proliferating CD64(+) M1 macrophages. Immunology and cell biology (2016) No. 94(5): 470-478 (

Huber, M., Epping, J., Schulze Gronover, C., Fricke, J., Aziz, Z., Brillatz, T., Swyers, M., Köllner, T.G., Vogel, H., Hammerbacher, A., Triebwasser-Freese, D., Robert, C.A.M., Verhoeven, K., Preite, V., Gershenzon, J., Erb, M.: A latex metabolite benefits plant fitness under root herbivore attack. PLoS Biology (2016) No. 14(1) (


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Keating, S.M., Heitman, J.W., Wu, S., Deng, X., Stacey, A.R., Zahn, R.C., de la Rosa, M., Finstad, S.L., Lifson, J.D., Piatak, M., Gauduin, M.C., Kessler, B.M., Ternette, N., Carville, A., Johnson, R.P., Desrosiers, R.C., Letvin, N.L., Borrow, P., Norris, P.J., Schmitz, J.E.: Magnitude and quality of cytokine and chemokine storm during acute infection distinguish non-progressive and progressive simian immunodeficiency virus infections of nonhuman primates. Journal of  Virology (2016) No. 90(22): 10339-10350 PMID: 27630228 (

Klose, D., Saunders, U., Barth, S., Fischer, R., Jacobi, A.M., Nachreiner, T.: Novel fusion proteins for the antigen-specific staining and elimination of B cell receptor-positive cell populations demonstrated by a tetanus toxoid fragment C (TTC) model antigen. Bmc Biotechnology (2016) No. 16: 18 (

Madeira, L.M., Szeto, T.H., Henquet, M., Raven, N., Runions, J., Huddleston, J., Garrard, I., Drake, P.M.W., Ma, J.K.-C.: High-yield production of a human monoclonal IgG by rhizosecretion in hydroponic tobacco cultures. Plant biotechnology journal 14 (2016) No. 2: 615-624 (

Mandal, M.K., Ahvari, H., Schillberg, S., Schiermeyer, A.: Tackling unwanted proteolysis in plant production hosts used for molecular farming. Frontiers in Plant Science (2016) No. 7: Art. 267 (

Maskus, D.J., Królik, M., Bethke, S., Spiegel, H., Kapelski, S., Seidel, M., Addai-Mensah, O., Reimann, A., Klockenbring, T., Barth, S., Fischer, R., Fendel, R.: Characterization of a novel inhibitory human monoclonal antibody directed against Plasmodium falciparum apical membrane antigen 1. Scientific Reports (2016) No. 6: Art. 39462 (

Menzel, S., Holland, T., Boes, A., Spiegel, H., et al.: Optimized blanching reduces the host cell protein content and substantially enhances the recovery and stability of two plant-derived malaria vaccine candidates. Frontiers in Plant Science (2016) No. 7: 1-15 (

Mladenov, R., Hristodorov, D., Cremer, C., Gresch, G., Grieger, E., Schenke, L., et al.: CD64-directed microtubule associated protein tau kills leukemic blasts ex vivo. Oncotarget. (2016) No. 7(41): 67166-67174 (

Niesen, J., Hehmann-Titt, G., Woitok, M., Fendel, R., Barth, S., Fischer, R., et al.: A novel fully-human cytolytic fusion protein based on granzyme B shows in vitro cytotoxicity and ex vivo binding to solid tumors overexpressing the epidermal growth factor receptor. Cancer letters. (2016) No. 374(2): 229-240 (

Niesen, J., Sack, M., Seidel, M., Fendel, R., Barth, S., Fischer, R., et al.: SNAP-tag technology: a useful tool to determine affinity constants and other functional parameters of novel antibody fragments. Bioconjugat Chemistry (2016) No. 27(8): 1931-1941 (


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Peschen, D., Schillberg, S., Fischer, R.: Antibody-mediated pathogen resistance in plants. Methods in Molecular Biology (2016) No. 1385: 273-291 (

Rasche, S., Herwartz, D., Schuster, F., Jabloka, N., Weber, A., Fischer, R., Schillberg, S.: More for less: Improving the biomass yield of a pear cell suspension culture by design of experiments. Scientific Reports (2016) No.6 : 23371(

Rasche, S., Schmitz, C., Jablonka, N., Schillberg, S.: LED-beleuchtung von Fußballrasen – Möglichkeiten, Chancen und Grenzen. European Journal of Turfgrass Science (2016) No. 4: 69–75 (

Raven, N., Schillberg, S., Rasche, S.: Plant cell-based recombinant antibody manufacturing with a 200 L orbitally shaken disposable bioreactor. Methods in Molecular Biology (2016) No. 1385: 161–172 (

Schinkel, H., Schillberg, S.: Genome editing: intellectual property and product development in plant biotechnology. Plant Cell Reports (2016) No. 7: 1487-1491(

Schmitz, C., Fritsch, L., Fischer, R., Schillberg, S., Rasche, S.: Statistical experimental designs for the production of secondary metabolites in plant cell suspension cultures. Biotechnology letters 38 (2016) No.12: 2007-2014 (

Schneider, K., Schiermeyer, A., Dolls, A., Koch, N., Herwartz, D., Kirchhoff, J., Fischer, R., Russell, S.M., Cao, Z., Corbin, D.R., Sastry-Dent, L., Ainley, W.M., Webb, S.R., Schinkel, H., Schillberg, S.: Targeted gene exchange in plant cells mediated by a zinc finger nuclease double cut. Plant Biotechnology Journal 14 (2016) No. 4: 1151-1160 (

Stolze, A., Wanke, A., van Deenen, N., Geyer, R., Prüfer, R., Schulze Gronover, C.: Development of rubber-enriched dandelion varieties by metabolic engineering of the inulin pathway. Plant Biotechnology Journal (2016) (


V – Z

Vasilev, N., Boccard, J., Lang, G., Grömping, U., Fischer, R., Goepfert, S., Rudaz, S., Schillberg, S.: Structured plant metabolomics for the simultaneous exploration of multiple factors. Scientific Reports (2016) No. 6: Art. 37390 (

Vasilev, N., Smales, C.M., Fischer, R., Schillberg S., Schiermeyer, A. (2016): Developments in the production of mucosal antibodies in plants. Biotechnology Advances (2016) No. 34 (2): 77-87 (

Visser, F., Müller, B., Rose, J., Prüfer, D., Noll, G.A.: Forizymes – functionalised artificial forisomes as a platform for the production and immobilisation of single enzymes and multienzyme complexes. Science Reports (2016) No. 6: 30839 (

von Felbert, V., Bauerschlag, D., Maass, N., Brautigam, K., Meinhold-Heerlein, I., Woitok, M., et al.: A specific photoimmunotheranostics agent to detect and eliminate skin cancer cells expressing EGFR. Journal of Cancer Research and Clinical Oncology (2016) No. 142(5): 1003-1011 (

Woitok, M., Klose, D., Niesen, J., Richter, W., Abbas, M., Stein, C., et al.: The efficient elimination of solid tumor cells by EGFR-specific and HER2-specific scFv-SNAP fusion proteins conjugated to benzylguanine-modified auristatin F. Cancer Letters. (2016) No. 381(2): 323-330 (

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Beiss, V., Spiegel, H., Boes, A., Kapelski, S., Scheuermayer, M., Edgue, G., Sack, M., Fendel, R., Reimann, A., Schillberg, S., Pradel, G., Fischer, R., 2015a. Heat-precipitation allows the efficient purification of a functional plant-derived malaria transmission-blocking vaccine candidate fusion protein. Biotechnol. Bioeng. 112, 1297–1305.

Beiss, V., Spiegel, H., Boes, A., Scheuermayer, M., Reimann, A., Schillberg, S., Fischer, R., 2015b. Plant expression and characterization of the transmission-blocking vaccine candidate PfGAP50. BMC Biotechnology 15, 108.

Boes, A., Spiegel, H., Edgue, G., Kapelski, S., Scheuermayer, M., Fendel, R., Remarque, E., Altmann, F., Maresch, D., Reimann, A., Pradel, G., Schillberg, S., Fischer, R., 2015a. Detailed functional characterization of glycosylated and nonglycosylated variants of malaria vaccine candidate PfAMA1 produced in Nicotiana benthamiana and analysis of growth inhibitory responses in rabbits. Plant Biotechnol J 13, 222–234.

Boes, A., Spiegel, H., Voepel, N., Edgue, G., Beiss, V., Kapelski, S., Fendel, R., Scheuermayer, M., Pradel, G., Bolscher, J.M., Behet, M.C., Dechering, K.J., Hermsen, C.C., Sauerwein, R.W., Schillberg, S., Reimann, A., Fischer, R., 2015b. Analysis of a Multi-component Multi-stage Malaria Vaccine Candidate--Tackling the Cocktail Challenge. PLoS ONE 10, e0131456.

Bortesi, L., Fischer, R., 2015. The CRISPR/Cas9 system for plant genome editing and beyond. Biotechnology Advances 33, 41–52.

Brehm, H., Hristodorov, D., Pardo, A., Mladenov, R., Niesen, J., Fischer, R., Tur, M.K., Barth, S., 2015. Targeted killing of rhabdomyosarcoma cells by a MAP-based human cytolytic fusion protein. Cancer Lett. 365, 149–155.

Buntru, M., Vogel, S., Stoff, K., Spiegel, H., Schillberg, S., 2015. A versatile coupled cell-free transcription–translation system based on tobacco BY-2 cell lysates. Biotechnol. Bioeng. 112, 867–878.

Buyel, J.F., 2015. Controlling the interplay between Agrobacterium tumefaciens and plants during the transient expression of proteins. Bioengineered 6, 242–244.

Buyel, J.F., 2015. Process Development Strategies in Plant Molecular Farming. Curr Pharm Biotechnol 16, 966–982.

Buyel, J.F., Buyel, J.J., Haase, C., Fischer, R., 2015a. The impact of Pseudomonas syringae type III effectors on transient protein expression in tobacco. Plant Biol (Stuttg) 17, 484–492.

Buyel, J.F., Gruchow, H.M., Fischer, R., 2015. Depth Filters Containing Diatomite Achieve More Efficient Particle Retention than Filters Solely Containing Cellulose Fibers. Front Plant Sci 6, 1134.

Buyel, J.F., Twyman, R.M., Fischer, R., 2015b. Extraction and downstream processing of plant-derived recombinant proteins. Biotechnol. Adv. 33, 902–913.

Cremer, C., Vierbuchen, T., Hein, L., Fischer, R., Barth, S., Nachreiner, T., 2015. Angiogenin mutants as novel effector molecules for the generation of fusion proteins with increased cytotoxic potential. J. Immunother. 38, 85–95.

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Epping, J., van Deenen, N., Niephaus, E., Stolze, A., Fricke, J., Huber, C., Eisenreich, W., Twyman, R.M., Prüfer, D., Schulze Gronover, C., 2015. A rubber transferase activator is necessary for natural rubber biosynthesis in dandelion. Nature Plants 1, 15048.

Faber, B.W., Hellwig, S., Houard, S., Havelange, N., Drossard, J., Mertens, H., Croon ,A., Byrne, R., van der Werff, N., van der Eijk, M., Thomas, A.W., Kocken, C.H.M. and Remarque, E.J.: Production, quality control, stability and pharmacotoxicity of a mixture of three cGMP-produced Diversity Covering Plasmodium falciparum AMA1 proteins, submitted

Fritsch, L., Fischer, R., Wambach, C., Dudek, M., Schillberg, S., Schröper, F., 2015. Next-generation sequencing is a robust strategy for the high-throughput detection of zygosity in transgenic maize. Transgenic Res. 24, 615–623.

Hristodorov, D., Mladenov, R., von Felbert, V., Huhn, M., Fischer, R., Barth, S., Thepen, T., 2015. Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies. MAbs 7, 853–862.

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Kapelski, S., Boes, A., Spiegel, H., de Almeida, M., Klockenbring, T., Reimann, A., Fischer, R., Barth, S., Fendel, R., 2015a. Fast track antibody V-gene rescue, recombinant expression in plants and characterization of a PfMSP4-specific antibody. Malar. J. 14, 50.

Kapelski, S., de Almeida, M., Fischer, R., Barth, S., Fendel, R., 2015b. Antimalarial activity of granzyme B and its targeted delivery by a granzyme B-single-chain Fv fusion protein. Antimicrob. Agents Chemother. 59, 669–672.

Laibach, N., Hillebrand, A., Twyman, R.M., Prüfer, D., Schulze Gronover, C., 2015. Identification of a Taraxacum brevicorniculatum rubber elongation factor protein that is localized on rubber particles and promotes rubber biosynthesis. Plant J. 82, 609–620.

Ma, J.K.-C., Drossard, J., Lewis, D., Altmann, F., Boyle, J., Christou, P., Cole, T., Dale, P., van Dolleweerd, C.J., Isitt, V., Katinger, D., Lobedan, M., Mertens, H., Paul, M.J., Rademacher, T., Sack, M., Hundleby, P.A.C., Stiegler, G., Stoger, E., Twyman, R.M., Vcelar, B., Fischer, R., 2015. Regulatory approval and a first-in-human phase I clinical trial of a monoclonal antibody produced in transgenic tobacco plants. Plant Biotechnol. J. 13, 1106–1120.

Maskus, D.J., Bethke, S., Seidel, M., Kapelski, S., Addai-Mensah, O., Boes, A., Edgü, G., Spiegel, H., Reimann, A., Fischer, R., Barth, S., Klockenbring, T., Fendel, R., 2015. Isolation, production and characterization of fully human monoclonal antibodies directed to Plasmodium falciparum MSP10. Malar. J. 14, 276.

Mladenov, R., Hristodorov, D., Cremer, C., Hein, L., Kreutzer, F., Stroisch, T., Niesen, J., Brehm, H., Blume, T., Brümmendorf, T.H., Jost, E., Thepen, T., Fischer, R., Stockmeyer, B., Barth, S., Stein, C., 2015. The Fc-alpha receptor is a new target antigen for immunotherapy of myeloid leukemia. Int. J. Cancer 137, 2729–2738.

Niesen, J., Brehm, H., Stein, C., Berges, N., Pardo, A., Fischer, R., Ten Haaf, A., Gattenlöhner, S., Tur, M.K., Barth, S., 2015a. In vitro effects and ex vivo binding of an EGFR-specific immunotoxin on rhabdomyosarcoma cells. J. Cancer Res. Clin. Oncol. 141, 1049–1061.

Niesen, J., Stein, C., Brehm, H., Hehmann-Titt, G., Fendel, R., Melmer, G., Fischer, R., Barth, S., 2015b. Novel EGFR-specific immunotoxins based on panitumumab and cetuximab show in vitro and ex vivo activity against different tumor entities. J. Cancer Res. Clin. Oncol. 141, 2079–2095.

P - U

Popovic, M., Prodanovic, R., Ostafe, R., Schillberg, S., Fischer, R., Gavrovic-Jankulovic, M., 2015. Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens. Immunol. Res. 61, 230–239.

Raven, N., Rasche, S., Kuehn, C., Anderlei, T., Klöckner, W., Schuster, F., Henquet, M., Bosch, D., Büchs, J., Fischer, R., Schillberg, S., 2015. Scaled-up manufacturing of recombinant antibodies produced by plant cells in a 200-L orbitally-shaken disposable bioreactor. Biotechnol. Bioeng. 112, 308–321.

Rettcher, S., Jungk, F., Kühn, C., Krause, H.-J., Nölke, G., Commandeur, U., Fischer, R., Schillberg, S., Schröper, F., 2015. A simple and portable magnetic immunoassay for the rapid detection and sensitive quantification of plant viruses. Appl. Environ. Microbiol. AEM.03667-14.

Sack, M., Hofbauer, A., Fischer, R., Stoger, E., 2015a. The increasing value of plant-made proteins. Curr. Opin. Biotechnol. 32, 163–170.

Sack, M., Rademacher, T., Spiegel, H., Boes, A., Hellwig, S., Drossard, J., Stoger, E., Fischer, R., 2015b. From gene to harvest: insights into upstream process development for the GMP production of a monoclonal antibody in transgenic tobacco plants. Plant Biotechnol. J. 13, 1094–1105.

Schubert, M., Houdelet, M., Kogel, K.-H., Fischer, R., Schillberg, S., Nölke, G., 2015. Thanatin confers partial resistance against aflatoxigenic fungi in maize (Zea mays). Transgenic Res. 24, 885–895.

Spiegel, H., Boes, A., Voepel, N., Beiss, V., Edgue, G., Rademacher, T., Sack, M., Schillberg, S., Reimann, A., Fischer, R., 2015. Application of a Scalable Plant Transient Gene Expression Platform for Malaria Vaccine Development. Front. Plant Sci. 1169.

A - C

Brehm, H., Niesen, J., Mladenov, R., Stein, C., Pardo, A., Fey, G., Helfrich, W., Fischer, R., Gattenlöhner, S., Barth, S., 2014. A CSPG4-specific immunotoxin kills rhabdomyosarcoma cells and binds to primary tumor tissues. Cancer Lett. 352, 228–235.

Buntru, M., Vogel, S., Spiegel, H., Schillberg, S., 2014. Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system. BMC Biotechnology 14, 37.

D - I

Havenith, H., Raven, N., Fiore, S.D., Fischer, R., Schillberg, S., 2014. Image-based analysis of cell-specific productivity for plant cell suspension cultures. Plant Cell Tiss Organ Cult 117, 393–399.

J - O

Kern, S., Agarwal, S., Huber, K., Gehring, A.P., Strödke, B., Wirth, C.C., Brügl, T., Abodo, L.O., Dandekar, T., Doerig, C., Fischer, R., Tobin, A.B., Alam, M.M., Bracher, F., Pradel, G., 2014. Inhibition of the SR Protein-Phosphorylating CLK Kinases of Plasmodium falciparum Impairs Blood Stage Replication and Malaria Transmission. PLoS One 9.

Mandal, M.K., Fischer, R., Schillberg, S., Schiermeyer, A., 2014. Inhibition of protease activity by antisense RNA improves recombinant protein production in Nicotiana tabacum cv. Bright Yellow 2 (BY-2) suspension cells. Biotechnol J 9, 1065–1073.

Müller, B., Groscurth, S., Menzel, M., Rüping, B.A., Twyman, R.M., Prüfer, D., Noll, G.A., 2014. Molecular and ultrastructural analysis of forisome subunits reveals the principles of forisome assembly. Ann. Bot. 113, 1121–1137.

Nölke, G., Houdelet, M., Kreuzaler, F., Peterhänsel, C., Schillberg, S., 2014. The expression of a recombinant glycolate dehydrogenase polyprotein in potato (Solanum tuberosum) plastids strongly enhances photosynthesis and tuber yield. Plant Biotechnol J 12, 734–742.

Ostafe, R., Prodanovic, R., Nazor, J., Fischer, R., 2014. Ultra-High-Throughput Screening Method for the Directed Evolution of Glucose Oxidase. Chemistry & Biology 21, 414–421.

P - U

Schramm, O.G., López-Cortés, X., Santos, L.S., Laurie, V.F., Nilo, F.D.G., Krolik, M., Fischer, R., Fiore, S.D., 2013. pH-dependent nano-capturing of tartaric acid using dendrimers. Soft Matter 10, 600–608.

Sommer, J., Garbers, C., Wolf, J., Trad, A., Moll, J.M., Sack, M., Fischer, R., Grötzinger, J., Waetzig, G.H., Floss, D.M., Scheller, J., 2014. Alternative intronic polyadenylation generates the interleukin-6 trans-signaling inhibitor sgp130-E10. J. Biol. Chem. 289, 22140–22150.

Stoger, E., Fischer, R., Moloney, M., Ma, J.K.-C., 2014. Plant molecular pharming for the treatment of chronic and infectious diseases. Annu Rev Plant Biol 65, 743–768.

V - Z

Vasilev, N., Schmitz, C., Grömping, U., Fischer, R., Schillberg, S., 2014. Assessment of cultivation factors that affect biomass and geraniol production in transgenic tobacco cell suspension cultures. PLoS ONE 9, e104620.

Voepel, N., Boes, A., Edgue, G., Beiss, V., Kapelski, S., Reimann, A., Schillberg, S., Pradel, G., Fendel, R., Scheuermayer, M., Spiegel, H., Fischer, R., 2014. Malaria vaccine candidate antigen targeting the pre-erythrocytic stage of Plasmodium falciparum produced at high level in plants. Biotechnol J 9, 1435–1445.

Wirth, C.C., Glushakova, S., Scheuermayer, M., Repnik, U., Garg, S., Schaack, D., Kachman, M.M., Weißbach, T., Zimmerberg, J., Dandekar, T., Griffiths, G., Chitnis, C.E., Singh, S., Fischer, R., Pradel, G., 2014. Perforin-like protein PPLP2 permeabilizes the red blood cell membrane during egress of Plasmodium falciparum gametocytes. Cell. Microbiol. 16, 709–733.

Zielonka, S., Ernst, A.M., Hawat, S., Twyman, R.M., Prüfer, D., Noll, G.A., 2014. Characterization of five subgroups of the sieve element occlusion gene family in Glycine max reveals genes encoding non-forisome P-proteins, forisomes and forisome tails. Plant Mol. Biol. 86, 51–67.