Fraunhofer Institute for Molecular Biology and Applied Ecology IMETutorial: transient production of recombinant proteins
Transient production of recombinant proteins using intact Nicotiana benthamiana plants and Agrobacterium tumefaciens in a process called Agroinfiltration is a powerful tool in research and development. The fast and robust method can yield up to 1mg protein per gram fresh leaf weight. In this video we show you how the plants can be efficiently infiltrated with Agrobacterium-suspension using a simple syringe and how to homogenize plant tissue to extract soluble proteins.