In collaboration with Wolfgang Eisenreich (TUM) and Richard Twyman (TRM), our Münster colleagues analyze cis-prenyltransferases, essential for linear isoprenoid biosynthesis. This study offers insights into the localization, interactions, and products of the T. koksaghyz cis-PT family, revealing a previously unknown pentaprenol exclusive to flowers. The findings will enable the selection of targets for future experiments focusing on primary and secondary polyisoprenoid metabolites. Importantly, our study underscores the specialized nature of the T. koksaghyz rubber transferase complex, paving the way for the rational design of improved enzymes.