The protection of soil and water requires the characterisation of their quality, the identification of relevant pollution pathways and the most comprehensive possible detection of potentially hazardous components in incoming material flows, such as composts, sewage sludge or wastewater. We are successively expanding our organic and inorganic chemical trace analytics and effect-based analysis to include suspect and non-target methods. Using high-resolution LC-MS/MS, we record the entire mass spectrum of occurring substances. Also, using transcriptomics and proteomics, we record the mechanisms of action of all material loads that are present in effect-relevant concentrations in eluates of soil, compost or sewage sludge, or in water or wastewater samples. The local and regional biodiversity is recorded via e-DNA determinations and can be checked for a correlation with the contaminated material.