Identification of inhibitors of SARS-CoV-2
Fraunhofer IME recently published a preprint article on a repurposing screen, which identified multiple inhibitors of SARS-CoV-2.
To identify possible candidates for fast progression towards clinical studies against SARS-CoV-2, we screened a well-defined collection (Fraunhofer IME repurposing collection) of 5632 compounds including 3488 compounds which have undergone clinical investigations (marketed drugs, phases 1 -3, and withdrawn) across 600 indications. Compounds were screened for their inhibition of viral induced cytotoxicity using the human epithelial colorectal adenocarcinoma cell line Caco-2 and a SARS-CoV-2 isolate. The primary screen of 5632 compounds gave 271 hits. A total of 64 compounds with IC50 <20 µM were identified, including 19 compounds with IC50 < 1 µM. Of this confirmed hit population, 90% have not yet been previously reported as active against SARS-CoV-2 in-vitro cell assays. Some 37 of the actives are launched drugs, 19 are in phases 1-3 and 10 pre-clinical. Several inhibitors were associated with modulation of host pathways including kinase signaling P53 activation, ubiquitin pathways and PDE activity modulation, with long chain acyl transferases were effective viral inhibitors.
The study was run by Fraunhofer IME in collaboration with the Goethe-University Frankfurt. For more details see the preprint article currently under review.
Bernhard Ellinger, Denisa Bojkova, Andrea Zaliani et al. Identification of inhibitors of SARS-CoV-2 in-vitro cellular toxicity in human (Caco-2) cells using a large scale drug repurposing collection, 20 April 2020, PREPRINT (Version 1)