DNA-sequencing is one of the most laborious and time consuming steps in the fast growing bio-businesses. It is required to characterize new genes and to validate the identity of your recombinant DNA. To assist you in your research efforts and to free up your valuable time we offer a complete portfolio of sequencing and related services:
- Plasmid DNA Preparation
- PCR-Product Purification
- Preparation of Sequencing Reactions
- Analysis of Sequencing Reactions
The 48-capillary 3730 DNA Analyzer is the gold standard in medium to high throughput genetic analysis with applications ranging from standard Sanger DNA sequencing to DNA fragment analysis such as microsatellites, AFLP, SNP analysis as well as mutation detection. For those applications, contact Dr. Jost Muth (0241 6085-12050).
With capillary electrophoresis (CE) good quality sequences usually reach a length of up to 800 clear bases under optimal conditions, while the first 50 bases of your template are not readable due to the limitations of CE. Sequencing primers should be chosen accordingly to cover the whole area of interest especially when sequencing long and short DNA fragments. Routine DNA sequencing is performed on Tuesdays and Thursdays with prices per samples of 4,50€ for less than 24 samples and 3,00€ for more than 24 samples, if handed in on a PCR plate. Sequencing results are given in standard .abi and .seq format and can be obtained from a password secured ftp server.