Fraunhofer Institute for Molecular Biology and Applied Ecology IMEIn the Bioprocess Engineering department, chromatographic separation processes are optimized for recombinant proteins by combining descriptive and mechanistic models. For this purpose, molecular descriptors such as total charge, surface charge and SMA parameter are used and includes chromatographic methods such as SEC, HIC, AEX, CEX, etc. In addition, non-chromatographic methods such as membrane, depth, ultra and diafiltration are also implemented in the extraction and purification protocols.
Purification
Chromatography columns coupled with an inherently fluorescent affinity ligand under ambient and fluorescent light.
© Fraunhofer IME
Model-based process development of extraction and purification methods
Development of mechanistic models for purification.
Mechanistic models for targeted chromatographic purification method.