The rational design of expression vectors and genes includes the optimization of the coding region, the regulatory elements of transcription and translation (e.g. promoters, UTRs, terminators) and the subcellular localization (cytosol, chloroplast, ER, vacuole, apoplast) as well as the combination with suitable fusion partners for the purification. The optimization is also tailored to the needs of process development and can include the directed modification of amino acids, for example, to increase protein expression, stability and purification.
Up to 100 different protein candidates can be analyzed in a semi-automated high-throughput process in microtiter plate format in one week.