In-vivo Pharmacology

Established animal models available within the IME-TMP cover a range of inflammatory, autoimmune, pain and neurodegenerative indications. Variables determined include direct functional, imaging and behavioral analyses, as well as ex-vivo immunohistological, immunological, cellular (e.g. FACS), receptor, mediator generating, enzyme, signaling, proteomic and genomic processes. This detailed assessment allows for distinctive phenotyping of drug effects.

Acute and inflammatory pain models

  • Mechanical and thermal pain thresholds (e.g. von Frey, dynamic plantar, Randall-Selitto cold/hot plate)
  • Inflammatory hyperalgesia (e.g. formalin, zymosan and adjuvant-induced)

Neuropathic pain models

  • Spared nerve injury
  • Chronic constriction injury (CCI)
  • Chemotherapy (paclitaxel or oxaliplatin-induced)

Animal models of neurodegenerative diseases

  • Experimental autoimmune encephalomyelitis (EAE)
  • Cognition and behavioral assessments

Acute and chronic inflammatory models

  • Zymosan and adjuvant-induced paw edema
  • Adjuvant-, antigen and collagen-induced arthritis
  • Cutaneous oxazolone contact sensitivity

Models of sepsis and vascular injury

  • Colon ligation sepsis
  • Endotoxin-induced sepsis

Animal models of autoimmune diseases

  • CYP2D6 mouse hepatitis
  • Bleomycin-induced skin fibrosis
  • Murine RIP-LCMV-induced type 1 diabetes
  • Streptozotocin (STZ)-induced type 1 diabetes

Animal models of tissue repair

Selected rodent models

For preclinical profiling of compounds we offer the use of:

  • Objective, sensitive and specific readouts of locomotor impairment, recovery or improvement
  • IVIS Spectrum (Caliper Life Sciences): optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression in living animals, applicable for e.g. inflammation, neurology, immunology and drug metabolism studies

Models for other indications are also available for specific interests.

 

Selected Publications

Schmitz K, de Bruin N, Bishay P, Männich J, Häussler A, Altmann C, et al., (2014) R-flurbiprofen attenuates experimental autoimmune encephalomyelitis in mice, EMBO Mol Med 6: 1398–1423. doi:10.15252/emmm.201404168.

de Bruin NMWJ., Schmitz K, Schiffmann S, Tafferner N, Schmidt M, Jordan H, et al. (2016) Multiple rodent models and behavioral measures reveal unexpected responses to FTY720 and DMF in experimental autoimmune encephalomyelitis, Behav Brain Res 300 (2016) 160–174. doi:10.1016/j.bbr.2015.12.006.

Parnham MJ, de Bruin N, Scholich K, Schmidt M, Jordan H, Geisslinger G (2014). Non-invasive bioluminescence imaging (BLI) of inflammation in murine allergic contact dermatitis. Proceedings of the British Pharmacological Society 12(3): abst033P http://www.pa2online.org/abstracts/1vol12issue3abst033p.pdf