We are identifying and characterizing targets for potential therapy of sepsis patients using cell culture in vitro, as well as the polymicrobial sepsis model, induced in the mouse by caecum ligation and puncture. With the cell culture systems, we have access to high-throughput screening methods which allow us to screen test compounds at transcriptional and translational levels. An emphasis is laid on methods which permit testing of compounds on intact cells. The data acquired in this way are validated by translation as rapidly as possible to the experimental model in vivo. Conversely, in-vivo findings are studied mechanistically in vitro.