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Bioaccumulation describes the enrichment of chemicals in organisms. The uptake of substances occurs either via food (biomagnification) or directly from the abiotic environment (bioconcentration). Experimentally determined bioaccumulation factors are an important element in the risk assessment of chemical substances. The basis for bioconcentration studies with fish is the OECD Guideline 305 (flow-through fish test).

Especially for highly lipophilic chemicals (log P > 5) bioconcentration studies are often difficult to perform. Lipophilic substances are poorly water soluble, which makes the adjustment of stable test concentrations difficult and, under certain conditions, may cause inexact measurements of the test substance in the medium. For poorly soluble chemicals an alternative test design for the performance of bioaccumulation studies based on feeding experiments is available in the scope of the revised OECD guideline 305. The aim of these studies is the determination of the biomagnification factor (BMF).


Goss, K.-U., Linden, L., Ulrich, N., Schlechtriem, C.:
Revisiting elimination half live as an indicator for bioaccumulation in fish and terrestrial mammals. Chemosphere, Vol 210, November 2018, Pages 341-346

Nichols, J., Fay, K., Bernhard, M.J., Bischof, I., Davis, J., Halder, M., Hu, J., Johanning, K., Laue, H., Nabb, D., Schlechtriem, C., Segner, H., Swintek, J., Weeks, J., Embry, M.:
Reliability of In Vitro Methods used to Measure Intrinsic Clearance of Hydrophobic Organic Chemicals by Fish: Results of an International Ring Trial. Toxicological Sciences, Volume 164, Issue 2, 2018, Pages 563–575

Kampe, S., Schlechtriem, C.:
Bioaccumulation of hexachlorobenzene in the terrestrial isopod Porcellio scaber. Environmental Toxicology and Chemistry, Online first (2016) (DOI: 10.1002/etc.3473)

Schlechtriem, C., Böhm, L., Bebon, R., Bruckert, H.-J., Düring, R.-A.:
Fish bioconcentration studies with column generated analyte concentrations of highly hydrophobic organic chemicals. Environmental Toxicology and Chemistry Accepted manuscript online (2016) (DOI: 10.1002/etc.3635)

Böhm, L., Schlechtriem, C., Düring, R.A.:
Sorption of highly hydrophobic chemicals to organic matter relevant for fish bioconcentration studies. Environmental Science & Technology 50 (15) (2016) 8316–8323 (DOI: 10.1021/acs.est.6b01778)

Brinkmann, M., Schlechtriem, C., Reininghaus, M., Eichbaum, K., Buchinger, S., Reifferscheid, G., Hollert, H., Preuss, T.:
Cross-species extrapolation of uptake and disposition of neutral organic chemicals using a multi-species physiologically based toxicokinetic model. Environmental Science & Technology 50 (4) (2016) 1914-1923 (DOI: 10.1021/acs.est.5b06158)

Treu, G., Drost, W., Jöhncke, U., Rauert, C., Schlechtriem, C.:
The Dessau Workshop on Bioaccumulation: State of the art, challenges and regulatory implications. Environmental Sciences Europe 27:34 (2015) (DOI: 10.1186/s12302-015-0067-0)

Schlechtriem, C., Kampe, S., Bruckert, H.J., Schaefers, C., L´Haridon, J..
Bioconcentration tests with fish and the freshwater amphipod Hyalella azteca. Are the results comparable? SETAC Europe 25th Annual Meeting, 3-7 May 2015, Barcelona, Catalonia, Spain

Goeritz, I., Falk, S., Stahl, T., Schäfers, C., Schlechtriem, C.:
Biomagnification and Tissue Distribution of perfluoroalkyl substances (PFASs) in market size rainbow trout (Oncorhynchus mykiss). Environmental Toxicology and Chemistry 32 (9) (2013) 2078–2088

Schlechtriem, C., Fliedner, A., and Schäfers, C.:
Determination of lipid content in fish samples from bioaccumulation studies: Contributions to the revision of guideline OECD 305. Environmental Sciences Europe (2012) 24:13

Düring, R-A., Böhm, L., Schlechtriem, C.:
Solid-phase microextraction for fish bioconcentration studies according to OECD TG 305. Environmental Sciences Europe (2012) 24: 4