Publications Molecular Biotechnology

Status 30.06.2018

Bröker, J.N., Müller, B., van Deenen, N., Prüfer, D., Schulze Gronover, C. Upregulating the mevalonate pathway and repressing sterol synthesis in Saccharomyces cerevisiae enhances the production of triterpenes. Applied Microbiology and Biotechnology 1-12 http://doi.org/DOI:10.1007/s00253-018-9154-7

Buyel, J.F. Plants as sources of natural and recombinant anti-cancer agents (2018) Biotechnology Advances, 36 (2), 506-520. http://doi.org/10.1016/j.biotechadv.2018.02.002

Gengenbach, B.B., Müschen, C.R., Buyel J.F. Expression and purification of human phosphatase and actin regulator 1 (PHACTR1) in plant-based systems (2018) Protein Expression and Purification, 151, 46-55 [Epub ahead of print] http://doi.org/10.1016/j.pep.2018.06.003

Hausmann, R., Chudobová, I., Spiegel, H., Schillberg, S. Proteomic analysis of CHO cell lines producing high and low quantities of a recombinant antibody before and after selection with methotrexate (2018) Journal of Biotechnology, 265, 65-69. https://doi.org/10.1016/j.jbiotec.2017.11.008

Holland, T., Buyel, J.F. Bioreactor-based production of glycoproteins in plant cell suspension cultures (2018) Methods in Molecular Biology, 1674, 129-146. http://doi.org/10.1007/978-1-4939-7312-5_11

Jansing, J., Buyel, J.F., The Correlation Between DsRed mRNA Levels and Transient DsRed Protein Expression in Plants Depends on Leaf Age and the 5' Untranslated Region (2018) Biotechnology Journal [Epub ahead of print] http://doi.org/10.1002/biot.201800075

Laibach, N., Schmidl, S., Müller, B., Bergmann, M., Prüfer, D., Schulze Gronover, C. Small rubber particle proteins from Taraxacum brevicorniculatum promote stress tolerance and influence the size and distribution of lipid droplets and artificial poly(cis-1,4-isoprene) bodies (2018) Plant Journal, 93 (6), 1045-1061. http://doi.org/10.1111/tpj.13829

McElroy, C., & Jennewein, S. (2018). Taxol® biosynthesis and production: from forests to fermenters. In Biotechnology of Natural Products (pp. 145-185). Springer, Cham. http://doi.org/10.1007/978-3-319-67903-7

Nölke, G., Barsoum, M., Houdelet, M., Arcalis, E., Kreuzaler, F., Fischer, R., Schillberg, S. The integration of algal carbon concentration mechanism components into tobacco chloroplasts increases photosynthetic efficiency and biomass (2018). Biotechnology Journal, 1800170. https://doi.org/10.1002/biot.201800170

Schubert, M., Spiegel, H., Schillberg, S., Nölke, G. Aspergillus-specific antibodies – Targets and applications (2018) Biotechnology Advances, 36 (4), 1167-1184. http://doi.org/10.1016/j.biotechadv.2018.03.016

Tyutereva, E.V., Dobryakova, K.S., Schiermeyer, A., Shishova, M.F., Pawlowski, K., Demidchik, V., Reumann, S., Voitsekhovskaja, O.V. The levels of peroxisomal catalase protein and activity modulate the onset of cell death in tobacco BY-2 cells via reactive oxygen species levels and autophagy (2018) Functional Plant Biology, 45 (1-2), 247-258. http://doi.org/10.1071/FP16418

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Akinrinmade, O.A., Jordaan, S., Hristodorov, D., Mladenov, R., Mungra, N., Chetty, S., Barth, S., 2017. Human MAP tau based targeted cytolytic fusion proteins. Biomedicines No. 5(3). http://doi.org/10.3390/biomedicines5030036

Augustine, S.M., 2017. CRISPR-cas9 system as a genome editing tool in sugarcane in: Mohan C. (Ed.) Sugarcane Biotechnology: Challenges and Prospects Springer International Publishing pp. 155-172. http://doi.org/10.1007/978-3-319-58946-6_11

Bauerschlag, D., Meinhold-Heerlein, I., Maass, N., Bleilevens, A., Bräutigam, K., Al Rawashdeh, W., Di Fiore, S., Haugg, A.M., Gremse, F., Steitz, J., Fischer, R., Stickeler, E., Barth, S., Hussain, A.F., 2017. Detection and Specific Elimination of EGFR+ Ovarian Cancer Cells Using a Near Infrared Photoimmunotheranostic Approach. Pharmaceutical Research 34, 696–703. http://doi.org/10.1007/s11095-017-2096-4

Berges, N., Arens, K., Kreusch, V., Fischer, R., Di Fiore, S., 2017. Toward discovery of novel microtubule targeting agents: A SNAP-tag-based high-content screening assay for the analysis of microtubule dynamics and cell cycle progression. SALAS Discovery 22, 387–398. http://doi.org/10.1177/2472555216685518

Braig, F., Kriegs, M., Voigtlaender, M., Habel, B., Grob, T., Biskup, K., Blanchard, V., Sack, M., Thalhammer, A., Batalla, I.B., Braren, I., Laban, S., Danielczyk, A., Goletz, S., Jakubowicz, E., Märkl, B., Trepel, M., Knecht, R., Riecken, K., Fehse, B., Loges, S., Bokemeyer, C., Binder, M., 2017. Cetuximab resistance in head and neck cancer is mediated by EGFR-K521 polymorphism. Cancer Research 77, 1188–1199. http://doi.org/10.1158/0008-5472.CAN-16-0754

Buyel, J.F., 2017a. Blanching facilitates the purification of recombinant proteins from plants. BioSpektrum 23, 522–524. http://doi.org/10.1007/s12268-017-0835-z

Buyel, J.F., 2017b. How plants can contribute to the supply of anticancer compounds, in: Biotechnology and Production of Anti-Cancer Compounds. pp. 39–72. http://doi.org/10.1007/978-3-319-53880-8_2

Buyel, J.F., Twyman, R.M., Fischer, R., 2017. Very-large-scale production of antibodies in plants: The biologization of manufacturing. Biotechnology Advances 35, 458–465. http://doi.org/10.1016/j.biotechadv.2017.03.011

Choudhary, S., Barth, S., Verma, R.S., 2017. SNAP-Tag Technology: A Promising Tool for Ex Vivo Immunophenotyping. Molecular Diagnosis and Therapy 21, 315–326. http://doi.org/10.1007/s40291-017-0263-2

Chouman, K., Woitok, M., Mladenov, R., Kessler, C., Weinhold, E., Hanz, G., Fischer, R., Meinhold-Heerlein, I., Bleilevens, A., Gresch, G., Haugg, A.M., Zeppernick, F., Bauerschlag, D., Maass, N., Stickeler, E., Kolberg, K., Hussain, A.F., 2017. Fine tuning antibody conjugation methods using SNAP-tag technology. Anti-Cancer Agents in Medicinal Chemistry 17, 1434–1440. http://doi.org/10.2174/1871520617666170213123737

Costa, M.P., Feitosa, A.C.S., Oliveira, F.C.E., Cavalcanti, B.C., Dias, G.G., Caetano, E.W.S., Sales, F.A.M., Freire, V.N., Di Fiore, S., Fischer, R., Ladeira, L.O., Da Silva Júnior, E.N., Pessoa, C., 2017. Encapsulation of nor-β-lapachone into poly(d,l)-lactide-: Co -glycolide (PLGA) microcapsules: Full characterization, computational details and cytotoxic activity against human cancer cell lines. MedChemComm 8, 1993–2002. http://doi.org/10.1039/c7md00196g

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Ece, S., Lambertz, C., Fischer, R., Commandeur, U., 2017. Heterologous expression of a Streptomyces cyaneus laccase for biomass modification applications. AMB Express 7. http://doi.org/10.1186/s13568-017-0387-0

Edgue, G., Twyman, R.M., Beiss, V., Fischer, R., Sack, M., 2017. Antibodies from plants for bionanomaterials. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology 9. http://doi.org/10.1002/wnan.1462

Fischer, R., Schillberg, S., 2017. Molecular Farming, in: Thomas, B., Murray, B.G., Murphy, D.J. (Eds.), Encyclopedia of Applied Plant Sciences (Second Edition). Academic Press, Oxford, pp. 77–82. http://doi.org/10.1016/B978-0-12-394807-6.00159-3

Gresch, G., Schenke, L., Mladenov, R., Zwirner, S., Cremer, C., Niesen, J., Grieger, E., Brümmendorf, T., Jost, E., Fischer, R., Stockmeyer, B., Barth, S., Nachreiner, T., Stein, C., 2017. Elimination of different leukaemia subtypes using novel CD89-specific human cytolytic fusion proteins. Br J Haematol n/a-n/a. http://doi.org/10.1111/bjh.14971

Grieger, E., Gresch, G., Niesen, J., Woitok, M., Barth, S., Fischer, R., Fendel, R., Stein, C., 2017. Efficient targeting of CD13 on cancer cells by the immunotoxin scFv13–ETA′ and the bispecific scFv [13xds16]. Journal of Cancer Research and Clinical Oncology 143, 2159–2170. http://doi.org/10.1007/s00432-017-2468-5

Havenith, H., Kern, K., Rautenberger, P., Spiegel, H., Szardenings, M., Ueberham, E., Lehmann, J., Buntru, M., Vogel, S., Treudler, R., Fischer, R., Schillberg, S., 2017. Combination of two epitope identification techniques enables the rational design of soy allergen Gly m 4 mutants. Biotechnology Journal 12. http://doi.org/10.1002/biot.201600441

Houdelet, M., Galinski, A., Holland, T., Wenzel, K., Schillberg, S., Buyel, J.F., 2017. Animal component-free Agrobacterium tumefaciens cultivation media for better GMP-compliance increases biomass yield and pharmaceutical protein expression in Nicotiana benthamiana. Biotechnology Journal 12. http://doi.org/10.1002/biot.201600721

Huck, N.V., Leissing, F., Majovsky, P., Buntru, M., Aretz, C., Flecken, M., Müller, J.P.J., Vogel, S., Schillberg, S., Hoehenwarter, W., Conrath, U., Beckers, G.J.M., 2017. Combined 15N-labeling and tandemmoac quantifies phosphorylation of map kinase substrates downstream of MKK7 in Arabidopsis. Frontiers in Plant Science No. 8. http://doi.org/10.3389/fpls.2017.02050

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Kastilan, R., Boes, A., Spiegel, H., Voepel, N., Chudobová, I., Hellwig, S., Buyel, J.F., Reimann, A., Fischer, R., 2017. Improvement of a fermentation process for the production of two PfAMA1-DiCo-based malaria vaccine candidates in Pichia pastoris. Scientific Reports 7. http://doi.org/10.1038/s41598-017-11819-4

Kessler, C., Pardo, A., Tur, M.K., Gattenlöhner, S., Fischer, R., Kolberg, K., Barth, S., 2017. Novel PSCA targeting scFv-fusion proteins for diagnosis and immunotherapy of prostate cancer. Journal of Cancer Research and Clinical Oncology 143, 2025–2038. http://doi.org/10.1007/s00432-017-2472-9

Klose, D., Woitok, M., Niesen, J., Beerli, R.R., Grawunder, U., Fischer, R., Barth, S., Fendel, R., Nachreiner, T.:
Generation of an artificial human B cell line test system using Transpo-mAbTM technology to evaluate the therapeutic efficacy of novel antigen-specific fusion proteins. PLoS ONE (2017) No. 12(7) http://doi.org/0.1371/journal.pone.0180305

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Purcell, O., Opdensteinen, P., Chen, W., Lowenhaupt, K., Brown, A., Hermann, M., Cao, J., Tenhaef, N., Kallweit, E., Kastilan, R., Sinskey, A.J., Perez-Pinera, P., Buyel, J.F., Lu, T.K., 2017. Production of Functional Anti-Ebola Antibodies in Pichia pastoris. ACS Synthetic Biology No. 6(12), 2183-2190. http.//doi.org/10.1021/acssynbio.7b00234

Pütter, K.M., van Deenen, N., Unland, K., Prüfer, D., Schulze Gronover, C., 2017. Isoprenoid biosynthesis in dandelion latex is enhanced by the overexpression of three key enzymes involved in the mevalonate pathway. BMC Plant Biology 17. http://doi.org/10.1186/s12870-017-1036-0

Schillberg, S., Raven, N., Fischer, R., Twyman, R.M., Schiermeyer, A., 2017. Contained Molecular Farming Using Plant Cell and Tissue Cultures, in: Yoshida, T. (Ed.), Applied Bioengineering. Wiley-VCH Verlag GmbH & Co. KGaA, pp. 259–281. http://doi.org/10.1002/9783527800599.ch9

Spiegel, H., Boes, A., Fendel, R., Reimann, A., Schillberg, S., Fischer, R., 2017. Immunization with the malaria diversity-covering blood-stage vaccine candidate plasmodium falciparum apical membrane antigen 1 DiCo in complex with its natural ligand PfRon2 does not improve the in vitro efficacy. Frontiers in Immunology 8. http://doi.org/10.3389/fimmu.2017.00743

Stolze, A., Wanke, A., van Deenen, N., Geyer, R., Prüfer, D., Schulze Gronover, C., 2017. Development of rubber-enriched dandelion varieties by metabolic engineering of the inulin pathway. Plant Biotechnology Journal 15, 740–753. http://doi.org/10.1111/pbi.12672

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Woitok, M., Klose, D., Di Fiore, S., Richter, W., Stein, C., Gresch, G., Grieger, E., Barth, S., Fischer, R., Kolberg, K., Niesen, J., 2017. Comparison of a mouse and a novel human scFv-SNAP-auristatin F drug conjugate with potent activity against EGFR-overexpressing human solid tumor cells. OncoTargets and Therapy 10, 3313–3327. http://doi.org/10.2147/OTT.S140492

Zhu, C., Bortesi, L., Baysal, C., Twyman, R.M., Fischer, R., Capell, T., Schillberg, S., Christou, P., 2017. Characteristics of Genome Editing Mutations in Cereal Crops. Trends in Plant Science 22, 38–52. http://doi.org/10.1016/j.tplants.2016.08.009

Zischewski, J., Fischer, R., Bortesi, L., 2017. Detection of on-target and off-target mutations generated by CRISPR/Cas9 and other sequence-specific nucleases. Biotechnology Advances 35, 95–104. http://doi.org/10.1016/j.biotechadv.2016.12.003

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Addai-Mensah, O., Seidel, M., Amidu, N., Maskus, D.J., Kapelski, S., Breuer, G., Franken, C., Owusu-Dabo, E., Frempong, M., Rakotozandrindrainy, R., Schinkel, H., Reimann, A., Klockenbring, T., Barth, S., Fischer, R., Fendel, R.: Acquired immune responses to three malaria vaccine candidates and their relationship to invasion inhibition in two populations naturally exposed to malaria. Malaria Journal. Online Journal (2016) No. 15: Art.65 (http://doi.org/10.1186/s12936-016-1112-1 )

Amoury, M., Bauerschlag, D., Zeppernick, F., Felbert, V., Berges, N., Di Fiore, S.D., Mintert, I., Bleilevens, A., Maass, N., Bräutigam, K., Meinhold-Heerlein, I., Stickeler, E., Barth, S., Fischer, R., Hussain, A.F.: Photoimmunotheranostic agents for triple-negative breast cancer diagnosis and therapy that can be activated on demand. OncoTarget 7 (2016), No. 34: 54925-54936 (http://doi.org/10.18632/oncotarget.10705)

Amoury, M., Kolberg, K., Pham, A.-T., Hristodorov, D., Mladenov, R., Di Fiore, S., Helfrich, W., Kiessling, F., Fischer, R., Pardo, A., Thepen, T., Hussain, A.F., Nachreiner, T., Barth, S.: Granzyme B-based cytolytic fusion protein targeting EpCAM specifically kills triple negative breast cancer cells in vitro and inhibits tumor growth in a subcutaneous mouse tumor model. Cancer letters 372 (2016) No. 2: 201-209 (http://doi.org/10.1016/j.canlet.2016.01.027)

Amoury, M., Mladenov, R., Nachreiner, T., Pham, A.-T., Hristodorov, D., Di Fiore, S., Helfrich, W., Pardo, A., Fey, G., Schwenkert, M., Thepen, T., Kiessling, F., Hussain, A.F., Fischer, R., Kolberg, K., Barth, S.: A novel approach for targeted elimination of CSPG4-positive triple-negative breast cancer cells using a MAP tau-based fusion protein. International Journal of Cancer 139 (2016) No. 4: 916-927 (http://doi.org/10.1002/ijc.30119)

Arfi, Z.A., Hellwig, S., Drossard, J., Fischer, R., Buyel, J.F.: Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisCO depletion and the removal of endotoxins. Biotechnology Journal 11 (2016) No. 4: 507-518 (http://doi.org/10.1002/biot.201500271)

Baysal, C., Bortesi, L., Zhu, C., Farré, G., Schillberg, S., Christou, P.: CRISPR/Cas9 activity in the rice OsBEIIb gene does not induce off-target effects in the closely related paralog OsBEIIa. Molecular Breeding 36 (2016) No. 8 (http://doi.org/10.1007/s11032-016-0533-4)

Bialon, M., Grezella, C., Friesen, L., Sieben, T., Pham, A.T., Fischer, R., et al.: A monoclonal antibody that discriminates between SNAP-Tagged and CLIP-tagged proteins. Monoclonal antibodies in immunodiagnosis and immunotherapy. 2016 No. 35(3): 141-147 (http://dx.doi.org/10.1089%2Fmab.2016.0002)

Bodewein, L., Schmelter, F., Di Fiore, S., Hollert, H., Fischer, R., Fenske, M.: Differences in toxicity of anionic and cationic PAMAM and PPI dendrimers in zebrafish embryos and cancer cell lines. Toxicology and Applied Pharmacology (2016) No. 395: 83-92 (http://doi.org/10.1016/j.taap.2016.06.008)

Boes, A., Reimann, A., Twyman, R.M., Fischer, R., Schillberg, S., Spiegel, H.: A plant-based transient expression system for the rapid production of malaria vaccine candidates. Methods in Molecular Biology (2016) No. 1404: 597-619 (http://doi.org/10.1007/978-1-4939-3389-1_39)

Boes, A., Spiegel, H., Kastilan, R., Bethke, S., Voepel, N., Chudobova, I., Bolscher, J.M., Dechering, K.J., Fendel, R., Buyel, J.F., Reimann, A., Schillberg, S., Fischer, R.: Analysis of the dose-dependent stage-specific in vitro efficacy of a multi-stage malaria vaccine candidate cocktail. Malaria Journal. Online Journal 15 (2016) Art. 279 (http://doi.org/10.1186/s12936-016-1328-0)

Boesch, A.W., Osei-Owusu, N.Y., Crowley, A.R., Chu, T.H., Chan, Y.N., Weiner, J.A., Bharadwaj, P., Hards, R., Adamo, M.E., Gerber, S.A., Cocklin, S.L., Schmitz, J.E., Miles, A.R., Eckman, J.W., Belli, A.J., Reimann, K.A., Ackerman, M.E.: Biophysical and functional characterization of rhesus macaque IgG subclasses.  Front Immunology (2016) (http://doi.org/10.3389/fimmu.2016.00589)

Bortesi, L., Zhu, C., Zischewski, J., Perez, L., Bassié, L., Nadi, R., Forni, G., Lade, S.B., Soto, E., Jin, X., Medina, V., Villorbina, G., Munoz, P., Farré, G., Fischer, R., Twyman, R.M., Capell, T., Christou, P., Schillberg, S.: Patterns of CRISPR/Cas9 activity in plants, animals and microbes. Plant biotechnology journal 14 (2016) No.12: 2203-2216 (http://doi.org/10.1111/pbi.12634)

Buyel, J.F.: Numeric simulation can be used to predict heat transfer during the blanching of leaves and intact plants. Biochemical Engineering Journal (2016) No. 109: 118-126 (http://doi.org/10.1016/j.bej.2016.01.009)

Buyel, J.F.: Procedure to evaluate the efficiency of flocculants for the removal of dispersed particles from plant extracts. Journal of Visualized Experiments: JoVE. Online resource (2016) No.110: Art. e53940 (http://doi.org/10.3791/53940)

Buyel, J.F., Gruchow, H.M., Tödter, N., Wehner, M.: Determination of the thermal properties of leaves by non-invasive contact free laser probing. Journal of Biotechnology (2016) No. 217: 100-108 (http://dx.doi.org/10.1016%2Fj.jbiotec.2015.11.008)

Buyel, J.F., Hubbuch, J., Fischer, R.: Comparison of tobacco host cell protein removal methods by blanching intact plants or by heat treatment of extracts. Journal of Visualized Experiments: JoVE. Online resource (2016) No.114 (http://doi.org/10.3791/54343)

Cartwright, E.K., Spicer, L., Smith, S.A., Lee, D., Fast, R., Paganini, S., Lawson, B.O., Nega, M., Easley, K., Schmitz, J.E., Bosinger, S.E., Paiardini, M., Chahroudi, A., Vanderford, T.H., Estes, J.D., Lifson, J.D., Derdeyn, C.A., Silvestri, G.: CD8+ lymphocytes are required for maintaining viral suppression in SIV-infected macaques treated with short-term antiretroviral therapy. Immunity. (2016) No. 45(3): 656-668 (http://doi.org/10.1016/j.immuni.2016.08.018. PMID: 27653601)

Carvajal-Rondanelli, P., Aróstica, M., Marshall, S.H., Albericio, F., Álvarez, C.A., Ojeda, C., Aguilar, L.F., Guzmán, F.: Inhibitory effect of short cationic homopeptides against Gram-negative bacteria. Amino Acids (2016) No. 48(6): 1445-1456 (http://doi.org/10.1007/s00726-016-2198-z)

Castro, R.I., Forero-Doria, O., Guzmán, L., Laurie, V.F., Valdés, O., Ávila-Salas, F., López-Cortés, X., Santos, L.S.: New polymer for removal of wine phenolics: Poly(N-(3-(N-isobutyrylisobutyramido)-3-oxopropyl)acrylamide) (P-NIOA). Food Chemistry (2016) No. 213:  554-560 (http://dx.doi.org/10.1016%2Fj.foodchem.2016.06.060)

Chan, Y.N., Boesch, A.W., Osei-Owusu, N.Y., Emileh, A., Crowley, A.R., Cocklin, S.L., Finstad, S.L., Linde, C.H., Howell, R.A., Zentner, I., Cocklin, S., Miles, A.R., Eckman, J.W., Alter, G., Schmitz, J.E., Ackerman, M.E.: IgG binding characteristics of rhesus macaque FcgR. Journal of Immunology (2016) No. 197(7): 2936-2947 (http://doi.org/10.4049/jimmunol.1502252. PMID: 27559046)

Choudhary, S., Pardo, A., Rosinke, R., Batra, J.K., Barth, S. Verma, R.S.: Targeting c-kit receptor in neuroblastomas and colorectal cancers using stem cell factor (SCF)-based recombinant bacterial toxins. Applied Microbiology and Biotechnology (2016) No. 100(1): 263-277 (http://dx.doi.org/10.1007%2Fs00253-015-6978-2)

Cong, X., Cremer, C., Nachreiner, T., Barth, S., Carloni, P.: Engineered human angiogenin mutations in the placental ribonuclease inhibitor complex for anticancer therapy: Insights from enhanced sampling simulations. Protein Science : a publication of the Protein Society (2016) No. 25(8): 1451-1460 (http://dx.doi.org/10.1002%2Fpro.2941)

Costa, M.P., Feitosa, A.C.S., Oliveira, F.C.E., Cavalcanti, B.C., Da Silva, E.N., Jr., Dias, G.G., Sales, F.A.M., Sousa, B.L., Barroso-Neto, I.L., Pessoa, C., Caetano, E.W.S., Di Fiore, S., Fischer, R., Ladeira, L.O., Freire, V.N.: Controlled release of Nor-b-lapachone by PLGA Microparticles: a strategy for improving cytotoxicity against prostate cancer cells. Molecules 21 (2016) No. 7: 12 S. (http://doi.org/10.3390/molecules21070873)

 

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Damm, T., Spiegel, H., Barth, S., Fischer, R., Naehring, J.: Development of a competitive cystatin C-Specific bioassay suitable for repetitivem measurements. PloS one. (2016) No. 11(1): e0147177 (http://dx.doi.org/10.1371%2Fjournal.pone.0147177)

Fischer, R., Schulze Gronover, C., Prüfer, D: Naturkautschuk aus russischem Löwenzahn. Ressourceneffizienz, Schlüsseltechnologie für Wirtschaft&Gesellschaft. Fraunhofer Forschungsfokus, Hrsg. Reimund Neugebauer (2016) 232-329  

Fritsch, L., Soeur, R., Hansen, C., Fischer, R., Schillberg, S., Schröper, F.: Next-generation sequencing of amplicons is a rapid and reliable method for the detection of polymorphisms relevant for barley breeding. Molecular Breeding 36 (2016) No.83 (http://doi.org/10.1007/s11032-016-0507-6)

Gaida, S.M., Liedtke, A., Jentges, A.H.W., Engels, B., Jennewein, S.: Metabolic engineering of clostridium cellulolyticum for the production of n-butanol from crystalline cellulose. Microbial Cell Factories. Online journal 15 (2016), Art.6 (http://doi.org/10.1186/s12934-015-0406-2)

Hristodorov, D., Mladenov, R., Fischer, R., Barth, S., Thepen, T.: Fully human MAP-fusion protein selectively targets and eliminates proliferating CD64(+) M1 macrophages. Immunology and cell biology (2016) No. 94(5): 470-478 (http://dx.doi.org/10.1038%2Ficb.2016.4)

Huber, M., Epping, J., Schulze Gronover, C., Fricke, J., Aziz, Z., Brillatz, T., Swyers, M., Köllner, T.G., Vogel, H., Hammerbacher, A., Triebwasser-Freese, D., Robert, C.A.M., Verhoeven, K., Preite, V., Gershenzon, J., Erb, M.: A latex metabolite benefits plant fitness under root herbivore attack. PLoS Biology (2016) No. 14(1) (http://doi.org/10.1371/journal.pbio.100233)

J – O

Keating, S.M., Heitman, J.W., Wu, S., Deng, X., Stacey, A.R., Zahn, R.C., de la Rosa, M., Finstad, S.L., Lifson, J.D., Piatak, M., Gauduin, M.C., Kessler, B.M., Ternette, N., Carville, A., Johnson, R.P., Desrosiers, R.C., Letvin, N.L., Borrow, P., Norris, P.J., Schmitz, J.E.: Magnitude and quality of cytokine and chemokine storm during acute infection distinguish non-progressive and progressive simian immunodeficiency virus infections of nonhuman primates. Journal of  Virology (2016) No. 90(22): 10339-10350 PMID: 27630228 (http://doi.org/10.1128/JVI.01061-16)

Klose, D., Saunders, U., Barth, S., Fischer, R., Jacobi, A.M., Nachreiner, T.: Novel fusion proteins for the antigen-specific staining and elimination of B cell receptor-positive cell populations demonstrated by a tetanus toxoid fragment C (TTC) model antigen. Bmc Biotechnology (2016) No. 16: 18 (http://dx.doi.org/10.1186%2Fs12896-016-0249-x)

Madeira, L.M., Szeto, T.H., Henquet, M., Raven, N., Runions, J., Huddleston, J., Garrard, I., Drake, P.M.W., Ma, J.K.-C.: High-yield production of a human monoclonal IgG by rhizosecretion in hydroponic tobacco cultures. Plant biotechnology journal 14 (2016) No. 2: 615-624 (http://doi.org/10.1111/pbi.12407)

Mandal, M.K., Ahvari, H., Schillberg, S., Schiermeyer, A.: Tackling unwanted proteolysis in plant production hosts used for molecular farming. Frontiers in Plant Science (2016) No. 7: Art. 267 (http://doi.org/10.3389/fpls.2016.00267)

Maskus, D.J., Królik, M., Bethke, S., Spiegel, H., Kapelski, S., Seidel, M., Addai-Mensah, O., Reimann, A., Klockenbring, T., Barth, S., Fischer, R., Fendel, R.: Characterization of a novel inhibitory human monoclonal antibody directed against Plasmodium falciparum apical membrane antigen 1. Scientific Reports (2016) No. 6: Art. 39462 (http://doi.org/10.1038/srep39462)

Menzel, S., Holland, T., Boes, A., Spiegel, H., et al.: Optimized blanching reduces the host cell protein content and substantially enhances the recovery and stability of two plant-derived malaria vaccine candidates. Frontiers in Plant Science (2016) No. 7: 1-15 (http://dx.doi.org/10.3389%2Ffpls.2016.00159)

Mladenov, R., Hristodorov, D., Cremer, C., Gresch, G., Grieger, E., Schenke, L., et al.: CD64-directed microtubule associated protein tau kills leukemic blasts ex vivo. Oncotarget. (2016) No. 7(41): 67166-67174 (http://doi.org/10.18632/oncotarget.11568)

Niesen, J., Hehmann-Titt, G., Woitok, M., Fendel, R., Barth, S., Fischer, R., et al.: A novel fully-human cytolytic fusion protein based on granzyme B shows in vitro cytotoxicity and ex vivo binding to solid tumors overexpressing the epidermal growth factor receptor. Cancer letters. (2016) No. 374(2): 229-240 (http://dx.doi.org/10.1016%2Fj.canlet.2016.02.020)

Niesen, J., Sack, M., Seidel, M., Fendel, R., Barth, S., Fischer, R., et al.: SNAP-tag technology: a useful tool to determine affinity constants and other functional parameters of novel antibody fragments. Bioconjugat Chemistry (2016) No. 27(8): 1931-1941 (http://doi.org/10.1021/acs.bioconjchem.6b00315)

 

P – U

Peschen, D., Schillberg, S., Fischer, R.: Antibody-mediated pathogen resistance in plants. Methods in Molecular Biology (2016) No. 1385: 273-291 (http://doi.org/10.1007/978-1-4939-3289-4_19)

Rasche, S., Herwartz, D., Schuster, F., Jabloka, N., Weber, A., Fischer, R., Schillberg, S.: More for less: Improving the biomass yield of a pear cell suspension culture by design of experiments. Scientific Reports (2016) No.6 : 23371(http://doi.org/10.1038/srep23371)

Rasche, S., Schmitz, C., Jablonka, N., Schillberg, S.: LED-beleuchtung von Fußballrasen – Möglichkeiten, Chancen und Grenzen. European Journal of Turfgrass Science (2016) No. 4: 69–75 (http://doi.org/10.1109/pesa.2006.343093)

Raven, N., Schillberg, S., Rasche, S.: Plant cell-based recombinant antibody manufacturing with a 200 L orbitally shaken disposable bioreactor. Methods in Molecular Biology (2016) No. 1385: 161–172 (http://doi.org/10.1007/978-1-4939-3289-4_12)

Schinkel, H., Schillberg, S.: Genome editing: intellectual property and product development in plant biotechnology. Plant Cell Reports (2016) No. 7: 1487-1491(http://doi.org/10.1007/s00299-016-1988-9)

Schmitz, C., Fritsch, L., Fischer, R., Schillberg, S., Rasche, S.: Statistical experimental designs for the production of secondary metabolites in plant cell suspension cultures. Biotechnology letters 38 (2016) No.12: 2007-2014 (http://doi.org/10.1007/s10529-016-2206-0)

Schneider, K., Schiermeyer, A., Dolls, A., Koch, N., Herwartz, D., Kirchhoff, J., Fischer, R., Russell, S.M., Cao, Z., Corbin, D.R., Sastry-Dent, L., Ainley, W.M., Webb, S.R., Schinkel, H., Schillberg, S.: Targeted gene exchange in plant cells mediated by a zinc finger nuclease double cut. Plant Biotechnology Journal 14 (2016) No. 4: 1151-1160 (http://doi.org/10.1111/pbi.12483)

Stolze, A., Wanke, A., van Deenen, N., Geyer, R., Prüfer, R., Schulze Gronover, C.: Development of rubber-enriched dandelion varieties by metabolic engineering of the inulin pathway. Plant Biotechnology Journal (2016) (http://doi.org/10.1111/pbi.12672)

 

V – Z

Vasilev, N., Boccard, J., Lang, G., Grömping, U., Fischer, R., Goepfert, S., Rudaz, S., Schillberg, S.: Structured plant metabolomics for the simultaneous exploration of multiple factors. Scientific Reports (2016) No. 6: Art. 37390 (http://doi.org/10.1038/srep37390)

Vasilev, N., Smales, C.M., Fischer, R., Schillberg S., Schiermeyer, A. (2016): Developments in the production of mucosal antibodies in plants. Biotechnology Advances (2016) No. 34 (2): 77-87 (http://doi.org/10.1016/j.biotechadv.2015.11.002)

Visser, F., Müller, B., Rose, J., Prüfer, D., Noll, G.A.: Forizymes – functionalised artificial forisomes as a platform for the production and immobilisation of single enzymes and multienzyme complexes. Science Reports (2016) No. 6: 30839 (http://doi.org/10.1038/srep30839)

von Felbert, V., Bauerschlag, D., Maass, N., Brautigam, K., Meinhold-Heerlein, I., Woitok, M., et al.: A specific photoimmunotheranostics agent to detect and eliminate skin cancer cells expressing EGFR. Journal of Cancer Research and Clinical Oncology (2016) No. 142(5): 1003-1011 (http://doi.org/10.1007/s00432-016-2122-7)

Woitok, M., Klose, D., Niesen, J., Richter, W., Abbas, M., Stein, C., et al.: The efficient elimination of solid tumor cells by EGFR-specific and HER2-specific scFv-SNAP fusion proteins conjugated to benzylguanine-modified auristatin F. Cancer Letters. (2016) No. 381(2): 323-330 (http://doi.org/10.1016/j.canlet.2016.08.003)

A - C

Beiss, V., Spiegel, H., Boes, A., Kapelski, S., Scheuermayer, M., Edgue, G., Sack, M., Fendel, R., Reimann, A., Schillberg, S., Pradel, G., Fischer, R., 2015a. Heat-precipitation allows the efficient purification of a functional plant-derived malaria transmission-blocking vaccine candidate fusion protein. Biotechnol. Bioeng. 112, 1297–1305. http://doi.org/10.1002/bit.25548

Beiss, V., Spiegel, H., Boes, A., Scheuermayer, M., Reimann, A., Schillberg, S., Fischer, R., 2015b. Plant expression and characterization of the transmission-blocking vaccine candidate PfGAP50. BMC Biotechnology 15, 108. http://doi.org/10.1186/s12896-015-0225-x

Boes, A., Spiegel, H., Edgue, G., Kapelski, S., Scheuermayer, M., Fendel, R., Remarque, E., Altmann, F., Maresch, D., Reimann, A., Pradel, G., Schillberg, S., Fischer, R., 2015a. Detailed functional characterization of glycosylated and nonglycosylated variants of malaria vaccine candidate PfAMA1 produced in Nicotiana benthamiana and analysis of growth inhibitory responses in rabbits. Plant Biotechnol J 13, 222–234. http://doi.org/10.1111/pbi.12255

Boes, A., Spiegel, H., Voepel, N., Edgue, G., Beiss, V., Kapelski, S., Fendel, R., Scheuermayer, M., Pradel, G., Bolscher, J.M., Behet, M.C., Dechering, K.J., Hermsen, C.C., Sauerwein, R.W., Schillberg, S., Reimann, A., Fischer, R., 2015b. Analysis of a Multi-component Multi-stage Malaria Vaccine Candidate--Tackling the Cocktail Challenge. PLoS ONE 10, e0131456. http://doi.org/10.1371/journal.pone.0131456

Bortesi, L., Fischer, R., 2015. The CRISPR/Cas9 system for plant genome editing and beyond. Biotechnology Advances 33, 41–52. http://doi.org/10.1016/j.biotechadv.2014.12.006

Brehm, H., Hristodorov, D., Pardo, A., Mladenov, R., Niesen, J., Fischer, R., Tur, M.K., Barth, S., 2015. Targeted killing of rhabdomyosarcoma cells by a MAP-based human cytolytic fusion protein. Cancer Lett. 365, 149–155. http://doi.org/10.1016/j.canlet.2015.04.004

Buntru, M., Vogel, S., Stoff, K., Spiegel, H., Schillberg, S., 2015. A versatile coupled cell-free transcription–translation system based on tobacco BY-2 cell lysates. Biotechnol. Bioeng. 112, 867–878. http://doi.org/10.1002/bit.25502

Buyel, J.F., 2015. Controlling the interplay between Agrobacterium tumefaciens and plants during the transient expression of proteins. Bioengineered 6, 242–244. http://doi.org/10.1080/21655979.2015.1052920

Buyel, J.F., 2015. Process Development Strategies in Plant Molecular Farming. Curr Pharm Biotechnol 16, 966–982. http://doi.org/10.2174/138920101611150902115413

Buyel, J.F., Buyel, J.J., Haase, C., Fischer, R., 2015a. The impact of Pseudomonas syringae type III effectors on transient protein expression in tobacco. Plant Biol (Stuttg) 17, 484–492. http://doi.org/10.1111/plb.12264

Buyel, J.F., Gruchow, H.M., Fischer, R., 2015. Depth Filters Containing Diatomite Achieve More Efficient Particle Retention than Filters Solely Containing Cellulose Fibers. Front Plant Sci 6, 1134. http://doi.org/10.3389/fpls.2015.01134

Buyel, J.F., Twyman, R.M., Fischer, R., 2015b. Extraction and downstream processing of plant-derived recombinant proteins. Biotechnol. Adv. 33, 902–913. http://doi.org/10.1016/j.biotechadv.2015.04.010

Cremer, C., Vierbuchen, T., Hein, L., Fischer, R., Barth, S., Nachreiner, T., 2015. Angiogenin mutants as novel effector molecules for the generation of fusion proteins with increased cytotoxic potential. J. Immunother. 38, 85–95. http://doi.org/10.1097/CJI.0000000000000053

D - I

Epping, J., van Deenen, N., Niephaus, E., Stolze, A., Fricke, J., Huber, C., Eisenreich, W., Twyman, R.M., Prüfer, D., Schulze Gronover, C., 2015. A rubber transferase activator is necessary for natural rubber biosynthesis in dandelion. Nature Plants 1, 15048. http://doi.org/10.1038/nplants.2015.48

Faber, B.W., Hellwig, S., Houard, S., Havelange, N., Drossard, J., Mertens, H., Croon ,A., Byrne, R., van der Werff, N., van der Eijk, M., Thomas, A.W., Kocken, C.H.M. and Remarque, E.J.: Production, quality control, stability and pharmacotoxicity of a mixture of three cGMP-produced Diversity Covering Plasmodium falciparum AMA1 proteins, submitted

Fritsch, L., Fischer, R., Wambach, C., Dudek, M., Schillberg, S., Schröper, F., 2015. Next-generation sequencing is a robust strategy for the high-throughput detection of zygosity in transgenic maize. Transgenic Res. 24, 615–623. http://doi.org/10.1007/s11248-015-9864-x

Hristodorov, D., Mladenov, R., von Felbert, V., Huhn, M., Fischer, R., Barth, S., Thepen, T., 2015. Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies. MAbs 7, 853–862. http://doi.org/10.1080/19420862.2015.1066950

J - O

Kapelski, S., Boes, A., Spiegel, H., de Almeida, M., Klockenbring, T., Reimann, A., Fischer, R., Barth, S., Fendel, R., 2015a. Fast track antibody V-gene rescue, recombinant expression in plants and characterization of a PfMSP4-specific antibody. Malar. J. 14, 50. http://doi.org/10.1186/s12936-015-0577-7

Kapelski, S., de Almeida, M., Fischer, R., Barth, S., Fendel, R., 2015b. Antimalarial activity of granzyme B and its targeted delivery by a granzyme B-single-chain Fv fusion protein. Antimicrob. Agents Chemother. 59, 669–672. http://doi.org/10.1128/AAC.04190-14

Laibach, N., Hillebrand, A., Twyman, R.M., Prüfer, D., Schulze Gronover, C., 2015. Identification of a Taraxacum brevicorniculatum rubber elongation factor protein that is localized on rubber particles and promotes rubber biosynthesis. Plant J. 82, 609–620. http://doi.org/10.1111/tpj.12836

Ma, J.K.-C., Drossard, J., Lewis, D., Altmann, F., Boyle, J., Christou, P., Cole, T., Dale, P., van Dolleweerd, C.J., Isitt, V., Katinger, D., Lobedan, M., Mertens, H., Paul, M.J., Rademacher, T., Sack, M., Hundleby, P.A.C., Stiegler, G., Stoger, E., Twyman, R.M., Vcelar, B., Fischer, R., 2015. Regulatory approval and a first-in-human phase I clinical trial of a monoclonal antibody produced in transgenic tobacco plants. Plant Biotechnol. J. 13, 1106–1120. http://doi.org/10.1111/pbi.12416

Maskus, D.J., Bethke, S., Seidel, M., Kapelski, S., Addai-Mensah, O., Boes, A., Edgü, G., Spiegel, H., Reimann, A., Fischer, R., Barth, S., Klockenbring, T., Fendel, R., 2015. Isolation, production and characterization of fully human monoclonal antibodies directed to Plasmodium falciparum MSP10. Malar. J. 14, 276. http://doi.org/10.1186/s12936-015-0797-x

Mladenov, R., Hristodorov, D., Cremer, C., Hein, L., Kreutzer, F., Stroisch, T., Niesen, J., Brehm, H., Blume, T., Brümmendorf, T.H., Jost, E., Thepen, T., Fischer, R., Stockmeyer, B., Barth, S., Stein, C., 2015. The Fc-alpha receptor is a new target antigen for immunotherapy of myeloid leukemia. Int. J. Cancer 137, 2729–2738. http://doi.org/10.1002/ijc.29628

Niesen, J., Brehm, H., Stein, C., Berges, N., Pardo, A., Fischer, R., Ten Haaf, A., Gattenlöhner, S., Tur, M.K., Barth, S., 2015a. In vitro effects and ex vivo binding of an EGFR-specific immunotoxin on rhabdomyosarcoma cells. J. Cancer Res. Clin. Oncol. 141, 1049–1061. http://doi.org/10.1007/s00432-014-1884-z

Niesen, J., Stein, C., Brehm, H., Hehmann-Titt, G., Fendel, R., Melmer, G., Fischer, R., Barth, S., 2015b. Novel EGFR-specific immunotoxins based on panitumumab and cetuximab show in vitro and ex vivo activity against different tumor entities. J. Cancer Res. Clin. Oncol. 141, 2079–2095. http://doi.org/10.1007/s00432-015-1975-5

P - U

Popovic, M., Prodanovic, R., Ostafe, R., Schillberg, S., Fischer, R., Gavrovic-Jankulovic, M., 2015. Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens. Immunol. Res. 61, 230–239. http://doi.org/10.1007/s12026-014-8614-0

Raven, N., Rasche, S., Kuehn, C., Anderlei, T., Klöckner, W., Schuster, F., Henquet, M., Bosch, D., Büchs, J., Fischer, R., Schillberg, S., 2015. Scaled-up manufacturing of recombinant antibodies produced by plant cells in a 200-L orbitally-shaken disposable bioreactor. Biotechnol. Bioeng. 112, 308–321. http://doi.org/10.1002/bit.25352

Rettcher, S., Jungk, F., Kühn, C., Krause, H.-J., Nölke, G., Commandeur, U., Fischer, R., Schillberg, S., Schröper, F., 2015. A simple and portable magnetic immunoassay for the rapid detection and sensitive quantification of plant viruses. Appl. Environ. Microbiol. AEM.03667-14. http://doi.org/10.1128/AEM.03667-14

Sack, M., Hofbauer, A., Fischer, R., Stoger, E., 2015a. The increasing value of plant-made proteins. Curr. Opin. Biotechnol. 32, 163–170. http://doi.org/10.1016/j.copbio.2014.12.008

Sack, M., Rademacher, T., Spiegel, H., Boes, A., Hellwig, S., Drossard, J., Stoger, E., Fischer, R., 2015b. From gene to harvest: insights into upstream process development for the GMP production of a monoclonal antibody in transgenic tobacco plants. Plant Biotechnol. J. 13, 1094–1105. http://doi.org/10.1111/pbi.12438

Schubert, M., Houdelet, M., Kogel, K.-H., Fischer, R., Schillberg, S., Nölke, G., 2015. Thanatin confers partial resistance against aflatoxigenic fungi in maize (Zea mays). Transgenic Res. 24, 885–895. http://doi.org/10.1007/s11248-015-9888-2

Spiegel, H., Boes, A., Voepel, N., Beiss, V., Edgue, G., Rademacher, T., Sack, M., Schillberg, S., Reimann, A., Fischer, R., 2015. Application of a Scalable Plant Transient Gene Expression Platform for Malaria Vaccine Development. Front. Plant Sci. 1169. http://doi.org/10.3389/fpls.2015.01169

A - C

Brehm, H., Niesen, J., Mladenov, R., Stein, C., Pardo, A., Fey, G., Helfrich, W., Fischer, R., Gattenlöhner, S., Barth, S., 2014. A CSPG4-specific immunotoxin kills rhabdomyosarcoma cells and binds to primary tumor tissues. Cancer Lett. 352, 228–235. http://doi.org/10.1016/j.canlet.2014.07.006

Buntru, M., Vogel, S., Spiegel, H., Schillberg, S., 2014. Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system. BMC Biotechnology 14, 37. http://doi.org/10.1186/1472-6750-14-37

D - I

Havenith, H., Raven, N., Fiore, S.D., Fischer, R., Schillberg, S., 2014. Image-based analysis of cell-specific productivity for plant cell suspension cultures. Plant Cell Tiss Organ Cult 117, 393–399. http://doi.org/10.1007/s11240-014-0448-x

J - O

Kern, S., Agarwal, S., Huber, K., Gehring, A.P., Strödke, B., Wirth, C.C., Brügl, T., Abodo, L.O., Dandekar, T., Doerig, C., Fischer, R., Tobin, A.B., Alam, M.M., Bracher, F., Pradel, G., 2014. Inhibition of the SR Protein-Phosphorylating CLK Kinases of Plasmodium falciparum Impairs Blood Stage Replication and Malaria Transmission. PLoS One 9. http://doi.org/10.1371/journal.pone.0105732

Mandal, M.K., Fischer, R., Schillberg, S., Schiermeyer, A., 2014. Inhibition of protease activity by antisense RNA improves recombinant protein production in Nicotiana tabacum cv. Bright Yellow 2 (BY-2) suspension cells. Biotechnol J 9, 1065–1073. http://doi.org/10.1002/biot.201300424

Müller, B., Groscurth, S., Menzel, M., Rüping, B.A., Twyman, R.M., Prüfer, D., Noll, G.A., 2014. Molecular and ultrastructural analysis of forisome subunits reveals the principles of forisome assembly. Ann. Bot. 113, 1121–1137. http://doi.org/10.1093/aob/mcu036

Nölke, G., Houdelet, M., Kreuzaler, F., Peterhänsel, C., Schillberg, S., 2014. The expression of a recombinant glycolate dehydrogenase polyprotein in potato (Solanum tuberosum) plastids strongly enhances photosynthesis and tuber yield. Plant Biotechnol J 12, 734–742. http://doi.org/10.1111/pbi.12178

Ostafe, R., Prodanovic, R., Nazor, J., Fischer, R., 2014. Ultra-High-Throughput Screening Method for the Directed Evolution of Glucose Oxidase. Chemistry & Biology 21, 414–421. http://doi.org/10.1016/j.chembiol.2014.01.010

P - U

Schramm, O.G., López-Cortés, X., Santos, L.S., Laurie, V.F., Nilo, F.D.G., Krolik, M., Fischer, R., Fiore, S.D., 2013. pH-dependent nano-capturing of tartaric acid using dendrimers. Soft Matter 10, 600–608. http://doi.org/10.1039/C3SM52255E

Sommer, J., Garbers, C., Wolf, J., Trad, A., Moll, J.M., Sack, M., Fischer, R., Grötzinger, J., Waetzig, G.H., Floss, D.M., Scheller, J., 2014. Alternative intronic polyadenylation generates the interleukin-6 trans-signaling inhibitor sgp130-E10. J. Biol. Chem. 289, 22140–22150. http://doi.org/10.1074/jbc.M114.560938

Stoger, E., Fischer, R., Moloney, M., Ma, J.K.-C., 2014. Plant molecular pharming for the treatment of chronic and infectious diseases. Annu Rev Plant Biol 65, 743–768. http://doi.org/10.1146/annurev-arplant-050213-035850

V - Z

Vasilev, N., Schmitz, C., Grömping, U., Fischer, R., Schillberg, S., 2014. Assessment of cultivation factors that affect biomass and geraniol production in transgenic tobacco cell suspension cultures. PLoS ONE 9, e104620. http://doi.org/10.1371/journal.pone.0104620

Voepel, N., Boes, A., Edgue, G., Beiss, V., Kapelski, S., Reimann, A., Schillberg, S., Pradel, G., Fendel, R., Scheuermayer, M., Spiegel, H., Fischer, R., 2014. Malaria vaccine candidate antigen targeting the pre-erythrocytic stage of Plasmodium falciparum produced at high level in plants. Biotechnol J 9, 1435–1445. http://doi.org/10.1002/biot.201400350

Wirth, C.C., Glushakova, S., Scheuermayer, M., Repnik, U., Garg, S., Schaack, D., Kachman, M.M., Weißbach, T., Zimmerberg, J., Dandekar, T., Griffiths, G., Chitnis, C.E., Singh, S., Fischer, R., Pradel, G., 2014. Perforin-like protein PPLP2 permeabilizes the red blood cell membrane during egress of Plasmodium falciparum gametocytes. Cell. Microbiol. 16, 709–733. http://doi.org/10.1111/cmi.12288

Zielonka, S., Ernst, A.M., Hawat, S., Twyman, R.M., Prüfer, D., Noll, G.A., 2014. Characterization of five subgroups of the sieve element occlusion gene family in Glycine max reveals genes encoding non-forisome P-proteins, forisomes and forisome tails. Plant Mol. Biol. 86, 51–67. http://doi.org/10.1007/s11103-014-0211-z