Veröffentlichungen Molekulare Biotechnologie

Vollständige Publikationslisten nach Jahren von 2009 bis 2015 können Sie in unseren Jahresberichten einsehen (für 2015 in den Annex Facts).

Jahresberichte

Stand August 2017

Bauerschlag, D., Meinhold-Heerlein, I., Maass, N., Bleilevens, A., Bräutigam, K., Al Rawashdeh, W., Di Fiore, S., Haugg, A.M., Gremse, F., Steitz, J., Fischer, R., Stickeler, E., Barth, S., Hussain, A.F., 2017. Detection and Specific Elimination of EGFR+ Ovarian Cancer Cells Using a Near Infrared Photoimmunotheranostic Approach. Pharmaceutical Research 34, 696–703. http://doi.org/10.1007/s11095-017-2096-4

Berges, N., Arens, K., Kreusch, V., Fischer, R., Di Fiore, S., 2017. Toward discovery of novel microtubule targeting agents: A SNAP-tag-based high-content screening assay for the analysis of microtubule dynamics and cell cycle progression. SALAS Discovery 22, 387–398. http://doi.org/10.1177/2472555216685518

Buyel, J.F., Twyman, R.M., Fischer, R., 2017. Very-large-scale production of antibodies in plants: The biologization of manufacturing. Biotechnology Advances 35, 458–465. http://doi.org/10.1016/j.biotechadv.2017.03.011

Ece, S., Lambertz, C., Fischer, R., Commandeur, U., 2017. Heterologous expression of a Streptomyces cyaneus laccase for biomass modification applications. AMB Express 7. http://doi.org/10.1186/s13568-017-0387-0

Fischer, R., Schillberg, S., 2017. Molecular Farming, in: Thomas, B., Murray, B.G., Murphy, D.J. (Eds.), Encyclopedia of Applied Plant Sciences (Second Edition). Academic Press, Oxford, pp. 77–82. http://doi.org/10.1016/B978-0-12-394807-6.00159-3

Havenith, H., Kern, K., Rautenberger, P., Spiegel, H., Szardenings, M., Ueberham, E., Lehmann, J., Buntru, M., Vogel, S., Treudler, R., Fischer, R., Schillberg, S., 2017. Combination of two epitope identification techniques enables the rational design of soy allergen Gly m 4 mutants. Biotechnology Journal 12. http://doi.org/10.1002/biot.201600441

Houdelet, M., Galinski, A., Holland, T., Wenzel, K., Schillberg, S., Buyel, J.F., 2017. Animal component-free Agrobacterium tumefaciens cultivation media for better GMP-compliance increases biomass yield and pharmaceutical protein expression in Nicotiana benthamiana. Biotechnology Journal 12. http://doi.org/10.1002/biot.201600721

Pütter, K.M., van Deenen, N., Unland, K., Prüfer, D., Schulze Gronover, C., 2017. Isoprenoid biosynthesis in dandelion latex is enhanced by the overexpression of three key enzymes involved in the mevalonate pathway. BMC Plant Biology 17. http://doi.org/10.1186/s12870-017-1036-0

Schillberg, S., Raven, N., Fischer, R., Twyman, R.M., Schiermeyer, A., 2017. Contained Molecular Farming Using Plant Cell and Tissue Cultures, in: Yoshida, T. (Ed.), Applied Bioengineering. Wiley-VCH Verlag GmbH & Co. KGaA, pp. 259–281. http://doi.org/10.1002/9783527800599.ch9

Spiegel, H., Boes, A., Fendel, R., Reimann, A., Schillberg, S., Fischer, R., 2017. Immunization with the malaria diversity-covering blood-stage vaccine candidate plasmodium falciparum apical membrane antigen 1 DiCo in complex with its natural ligand PfRon2 does not improve the in vitro efficacy. Frontiers in Immunology 8. http://doi.org/10.3389/fimmu.2017.00743

Stolze, A., Wanke, A., van Deenen, N., Geyer, R., Prüfer, D., Schulze Gronover, C., 2017. Development of rubber-enriched dandelion varieties by metabolic engineering of the inulin pathway. Plant Biotechnology Journal 15, 740–753. http://doi.org/10.1111/pbi.12672

Woitok, M., Klose, D., Di Fiore, S., Richter, W., Stein, C., Gresch, G., Grieger, E., Barth, S., Fischer, R., Kolberg, K., Niesen, J., 2017. Comparison of a mouse and a novel human scFv-SNAP-auristatin F drug conjugate with potent activity against EGFR-overexpressing human solid tumor cells. OncoTargets and Therapy 10, 3313–3327. http://doi.org/10.2147/OTT.S140492

Zhu, C., Bortesi, L., Baysal, C., Twyman, R.M., Fischer, R., Capell, T., Schillberg, S., Christou, P., 2017. Characteristics of Genome Editing Mutations in Cereal Crops. Trends in Plant Science 22, 38–52. http://doi.org/10.1016/j.tplants.2016.08.009

Zischewski, J., Fischer, R., Bortesi, L., 2017. Detection of on-target and off-target mutations generated by CRISPR/Cas9 and other sequence-specific nucleases. Biotechnology Advances 35, 95–104. http://doi.org/10.1016/j.biotechadv.2016.12.003

 

A-C

Addai-Mensah, O., Seidel, M., Amidu, N., Maskus, D.J., Kapelski, S., Breuer, G., Franken, C., Owusu-Dabo, E., Frempong, M., Rakotozandrindrainy, R., Schinkel, H., Reimann, A., Klockenbring, T., Barth, S., Fischer, R., Fendel, R.: Acquired immune responses to three malaria vaccine candidates and their relationship to invasion inhibition in two populations naturally exposed to malaria. Malaria Journal. Online Journal (2016) No. 15: Art.65 (http://doi.org/10.1186/s12936-016-1112-1 )

Amoury, M., Bauerschlag, D., Zeppernick, F., Felbert, V., Berges, N., Di Fiore, S.D., Mintert, I., Bleilevens, A., Maass, N., Bräutigam, K., Meinhold-Heerlein, I., Stickeler, E., Barth, S., Fischer, R., Hussain, A.F.: Photoimmunotheranostic agents for triple-negative breast cancer diagnosis and therapy that can be activated on demand. OncoTarget 7 (2016), No. 34: 54925-54936 (http://doi.org/10.18632/oncotarget.10705)

Amoury, M., Kolberg, K., Pham, A.-T., Hristodorov, D., Mladenov, R., Di Fiore, S., Helfrich, W., Kiessling, F., Fischer, R., Pardo, A., Thepen, T., Hussain, A.F., Nachreiner, T., Barth, S.: Granzyme B-based cytolytic fusion protein targeting EpCAM specifically kills triple negative breast cancer cells in vitro and inhibits tumor growth in a subcutaneous mouse tumor model. Cancer letters 372 (2016) No. 2: 201-209 (http://doi.org/10.1016/j.canlet.2016.01.027)

Amoury, M., Mladenov, R., Nachreiner, T., Pham, A.-T., Hristodorov, D., Di Fiore, S., Helfrich, W., Pardo, A., Fey, G., Schwenkert, M., Thepen, T., Kiessling, F., Hussain, A.F., Fischer, R., Kolberg, K., Barth, S.: A novel approach for targeted elimination of CSPG4-positive triple-negative breast cancer cells using a MAP tau-based fusion protein. International Journal of Cancer 139 (2016) No. 4: 916-927 (http://doi.org/10.1002/ijc.30119)

Arfi, Z.A., Hellwig, S., Drossard, J., Fischer, R., Buyel, J.F.: Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisCO depletion and the removal of endotoxins. Biotechnology Journal 11 (2016) No. 4: 507-518 (http://doi.org/10.1002/biot.201500271)

Baysal, C., Bortesi, L., Zhu, C., Farré, G., Schillberg, S., Christou, P.: CRISPR/Cas9 activity in the rice OsBEIIb gene does not induce off-target effects in the closely related paralog OsBEIIa. Molecular Breeding 36 (2016) No. 8 (http://doi.org/10.1007/s11032-016-0533-4)

Bialon, M., Grezella, C., Friesen, L., Sieben, T., Pham, A.T., Fischer, R., et al.: A monoclonal antibody that discriminates between SNAP-Tagged and CLIP-tagged proteins. Monoclonal antibodies in immunodiagnosis and immunotherapy. 2016 No. 35(3): 141-147 (http://dx.doi.org/10.1089%2Fmab.2016.0002)

Bodewein, L., Schmelter, F., Di Fiore, S., Hollert, H., Fischer, R., Fenske, M.: Differences in toxicity of anionic and cationic PAMAM and PPI dendrimers in zebrafish embryos and cancer cell lines. Toxicology and Applied Pharmacology (2016) No. 395: 83-92 (http://doi.org/10.1016/j.taap.2016.06.008)

Boes, A., Reimann, A., Twyman, R.M., Fischer, R., Schillberg, S., Spiegel, H.: A plant-based transient expression system for the rapid production of malaria vaccine candidates. Methods in Molecular Biology (2016) No. 1404: 597-619 (http://doi.org/10.1007/978-1-4939-3389-1_39)

Boes, A., Spiegel, H., Kastilan, R., Bethke, S., Voepel, N., Chudobova, I., Bolscher, J.M., Dechering, K.J., Fendel, R., Buyel, J.F., Reimann, A., Schillberg, S., Fischer, R.: Analysis of the dose-dependent stage-specific in vitro efficacy of a multi-stage malaria vaccine candidate cocktail. Malaria Journal. Online Journal 15 (2016) Art. 279 (http://doi.org/10.1186/s12936-016-1328-0)

Boesch, A.W., Osei-Owusu, N.Y., Crowley, A.R., Chu, T.H., Chan, Y.N., Weiner, J.A., Bharadwaj, P., Hards, R., Adamo, M.E., Gerber, S.A., Cocklin, S.L., Schmitz, J.E., Miles, A.R., Eckman, J.W., Belli, A.J., Reimann, K.A., Ackerman, M.E.: Biophysical and functional characterization of rhesus macaque IgG subclasses.  Front Immunology (2016) (http://doi.org/10.3389/fimmu.2016.00589)

Bortesi, L., Zhu, C., Zischewski, J., Perez, L., Bassié, L., Nadi, R., Forni, G., Lade, S.B., Soto, E., Jin, X., Medina, V., Villorbina, G., Munoz, P., Farré, G., Fischer, R., Twyman, R.M., Capell, T., Christou, P., Schillberg, S.: Patterns of CRISPR/Cas9 activity in plants, animals and microbes. Plant biotechnology journal 14 (2016) No.12: 2203-2216 (http://doi.org/10.1111/pbi.12634)

Buyel, J.F.: Numeric simulation can be used to predict heat transfer during the blanching of leaves and intact plants. Biochemical Engineering Journal (2016) No. 109: 118-126 (http://doi.org/10.1016/j.bej.2016.01.009)

Buyel, J.F.: Procedure to evaluate the efficiency of flocculants for the removal of dispersed particles from plant extracts. Journal of Visualized Experiments: JoVE. Online resource (2016) No.110: Art. e53940 (http://doi.org/10.3791/53940)

Buyel, J.F., Gruchow, H.M., Tödter, N., Wehner, M.: Determination of the thermal properties of leaves by non-invasive contact free laser probing. Journal of Biotechnology (2016) No. 217: 100-108 (http://dx.doi.org/10.1016%2Fj.jbiotec.2015.11.008)

Buyel, J.F., Hubbuch, J., Fischer, R.: Comparison of tobacco host cell protein removal methods by blanching intact plants or by heat treatment of extracts. Journal of Visualized Experiments: JoVE. Online resource (2016) No.114 (http://doi.org/10.3791/54343)

Cartwright, E.K., Spicer, L., Smith, S.A., Lee, D., Fast, R., Paganini, S., Lawson, B.O., Nega, M., Easley, K., Schmitz, J.E., Bosinger, S.E., Paiardini, M., Chahroudi, A., Vanderford, T.H., Estes, J.D., Lifson, J.D., Derdeyn, C.A., Silvestri, G.: CD8+ lymphocytes are required for maintaining viral suppression in SIV-infected macaques treated with short-term antiretroviral therapy. Immunity. (2016) No. 45(3): 656-668 (http://doi.org/10.1016/j.immuni.2016.08.018. PMID: 27653601)

Carvajal-Rondanelli, P., Aróstica, M., Marshall, S.H., Albericio, F., Álvarez, C.A., Ojeda, C., Aguilar, L.F., Guzmán, F.: Inhibitory effect of short cationic homopeptides against Gram-negative bacteria. Amino Acids (2016) No. 48(6): 1445-1456 (http://doi.org/10.1007/s00726-016-2198-z)

Castro, R.I., Forero-Doria, O., Guzmán, L., Laurie, V.F., Valdés, O., Ávila-Salas, F., López-Cortés, X., Santos, L.S.: New polymer for removal of wine phenolics: Poly(N-(3-(N-isobutyrylisobutyramido)-3-oxopropyl)acrylamide) (P-NIOA). Food Chemistry (2016) No. 213:  554-560 (http://dx.doi.org/10.1016%2Fj.foodchem.2016.06.060)

Chan, Y.N., Boesch, A.W., Osei-Owusu, N.Y., Emileh, A., Crowley, A.R., Cocklin, S.L., Finstad, S.L., Linde, C.H., Howell, R.A., Zentner, I., Cocklin, S., Miles, A.R., Eckman, J.W., Alter, G., Schmitz, J.E., Ackerman, M.E.: IgG binding characteristics of rhesus macaque FcgR. Journal of Immunology (2016) No. 197(7): 2936-2947 (http://doi.org/10.4049/jimmunol.1502252. PMID: 27559046)

Choudhary, S., Pardo, A., Rosinke, R., Batra, J.K., Barth, S. Verma, R.S.: Targeting c-kit receptor in neuroblastomas and colorectal cancers using stem cell factor (SCF)-based recombinant bacterial toxins. Applied Microbiology and Biotechnology (2016) No. 100(1): 263-277 (http://dx.doi.org/10.1007%2Fs00253-015-6978-2)

Cong, X., Cremer, C., Nachreiner, T., Barth, S., Carloni, P.: Engineered human angiogenin mutations in the placental ribonuclease inhibitor complex for anticancer therapy: Insights from enhanced sampling simulations. Protein Science : a publication of the Protein Society (2016) No. 25(8): 1451-1460 (http://dx.doi.org/10.1002%2Fpro.2941)

Costa, M.P., Feitosa, A.C.S., Oliveira, F.C.E., Cavalcanti, B.C., Da Silva, E.N., Jr., Dias, G.G., Sales, F.A.M., Sousa, B.L., Barroso-Neto, I.L., Pessoa, C., Caetano, E.W.S., Di Fiore, S., Fischer, R., Ladeira, L.O., Freire, V.N.: Controlled release of Nor-b-lapachone by PLGA Microparticles: a strategy for improving cytotoxicity against prostate cancer cells. Molecules 21 (2016) No. 7: 12 S. (http://doi.org/10.3390/molecules21070873)

 

D – I

Damm, T., Spiegel, H., Barth, S., Fischer, R., Naehring, J.: Development of a competitive cystatin C-Specific bioassay suitable for repetitivem measurements. PloS one. (2016) No. 11(1): e0147177 (http://dx.doi.org/10.1371%2Fjournal.pone.0147177)

Fischer, R., Schulze Gronover, C., Prüfer, D: Naturkautschuk aus russischem Löwenzahn. Ressourceneffizienz, Schlüsseltechnologie für Wirtschaft&Gesellschaft. Fraunhofer Forschungsfokus, Hrsg. Reimund Neugebauer (2016) 232-329  

Fritsch, L., Soeur, R., Hansen, C., Fischer, R., Schillberg, S., Schröper, F.: Next-generation sequencing of amplicons is a rapid and reliable method for the detection of polymorphisms relevant for barley breeding. Molecular Breeding 36 (2016) No.83 (http://doi.org/10.1007/s11032-016-0507-6)

Gaida, S.M., Liedtke, A., Jentges, A.H.W., Engels, B., Jennewein, S.: Metabolic engineering of clostridium cellulolyticum for the production of n-butanol from crystalline cellulose. Microbial Cell Factories. Online journal 15 (2016), Art.6 (http://doi.org/10.1186/s12934-015-0406-2)

Hristodorov, D., Mladenov, R., Fischer, R., Barth, S., Thepen, T.: Fully human MAP-fusion protein selectively targets and eliminates proliferating CD64(+) M1 macrophages. Immunology and cell biology (2016) No. 94(5): 470-478 (http://dx.doi.org/10.1038%2Ficb.2016.4)

Huber, M., Epping, J., Schulze Gronover, C., Fricke, J., Aziz, Z., Brillatz, T., Swyers, M., Köllner, T.G., Vogel, H., Hammerbacher, A., Triebwasser-Freese, D., Robert, C.A.M., Verhoeven, K., Preite, V., Gershenzon, J., Erb, M.: A latex metabolite benefits plant fitness under root herbivore attack. PLoS Biology (2016) No. 14(1) (http://doi.org/10.1371/journal.pbio.100233)

 

J – O

Keating, S.M., Heitman, J.W., Wu, S., Deng, X., Stacey, A.R., Zahn, R.C., de la Rosa, M., Finstad, S.L., Lifson, J.D., Piatak, M., Gauduin, M.C., Kessler, B.M., Ternette, N., Carville, A., Johnson, R.P., Desrosiers, R.C., Letvin, N.L., Borrow, P., Norris, P.J., Schmitz, J.E.: Magnitude and quality of cytokine and chemokine storm during acute infection distinguish non-progressive and progressive simian immunodeficiency virus infections of nonhuman primates. Journal of  Virology (2016) No. 90(22): 10339-10350 PMID: 27630228 (http://doi.org/10.1128/JVI.01061-16)

Klose, D., Saunders, U., Barth, S., Fischer, R., Jacobi, A.M., Nachreiner, T.: Novel fusion proteins for the antigen-specific staining and elimination of B cell receptor-positive cell populations demonstrated by a tetanus toxoid fragment C (TTC) model antigen. Bmc Biotechnology (2016) No. 16: 18 (http://dx.doi.org/10.1186%2Fs12896-016-0249-x)

Madeira, L.M., Szeto, T.H., Henquet, M., Raven, N., Runions, J., Huddleston, J., Garrard, I., Drake, P.M.W., Ma, J.K.-C.: High-yield production of a human monoclonal IgG by rhizosecretion in hydroponic tobacco cultures. Plant biotechnology journal 14 (2016) No. 2: 615-624 (http://doi.org/10.1111/pbi.12407)

Mandal, M.K., Ahvari, H., Schillberg, S., Schiermeyer, A.: Tackling unwanted proteolysis in plant production hosts used for molecular farming. Frontiers in Plant Science (2016) No. 7: Art. 267 (http://doi.org/10.3389/fpls.2016.00267)

Maskus, D.J., Królik, M., Bethke, S., Spiegel, H., Kapelski, S., Seidel, M., Addai-Mensah, O., Reimann, A., Klockenbring, T., Barth, S., Fischer, R., Fendel, R.: Characterization of a novel inhibitory human monoclonal antibody directed against Plasmodium falciparum apical membrane antigen 1. Scientific Reports (2016) No. 6: Art. 39462 (http://doi.org/10.1038/srep39462)

Menzel, S., Holland, T., Boes, A., Spiegel, H., et al.: Optimized blanching reduces the host cell protein content and substantially enhances the recovery and stability of two plant-derived malaria vaccine candidates. Frontiers in Plant Science (2016) No. 7: 1-15 (http://dx.doi.org/10.3389%2Ffpls.2016.00159)

Mladenov, R., Hristodorov, D., Cremer, C., Gresch, G., Grieger, E., Schenke, L., et al.: CD64-directed microtubule associated protein tau kills leukemic blasts ex vivo. Oncotarget. (2016) No. 7(41): 67166-67174 (http://doi.org/10.18632/oncotarget.11568)

Niesen, J., Hehmann-Titt, G., Woitok, M., Fendel, R., Barth, S., Fischer, R., et al.: A novel fully-human cytolytic fusion protein based on granzyme B shows in vitro cytotoxicity and ex vivo binding to solid tumors overexpressing the epidermal growth factor receptor. Cancer letters. (2016) No. 374(2): 229-240 (http://dx.doi.org/10.1016%2Fj.canlet.2016.02.020)

Niesen, J., Sack, M., Seidel, M., Fendel, R., Barth, S., Fischer, R., et al.: SNAP-tag technology: a useful tool to determine affinity constants and other functional parameters of novel antibody fragments. Bioconjugat Chemistry (2016) No. 27(8): 1931-1941 (http://doi.org/10.1021/acs.bioconjchem.6b00315)

 

P – U

Peschen, D., Schillberg, S., Fischer, R.: Antibody-mediated pathogen resistance in plants. Methods in Molecular Biology (2016) No. 1385: 273-291 (http://doi.org/10.1007/978-1-4939-3289-4_19)

Rasche, S., Herwartz, D., Schuster, F., Jabloka, N., Weber, A., Fischer, R., Schillberg, S.: More for less: Improving the biomass yield of a pear cell suspension culture by design of experiments. Scientific Reports (2016) No.6 : 23371(http://doi.org/10.1038/srep23371)

Rasche, S., Schmitz, C., Jablonka, N., Schillberg, S.: LED-beleuchtung von Fußballrasen – Möglichkeiten, Chancen und Grenzen. European Journal of Turfgrass Science (2016) No. 4: 69–75 (http://doi.org/10.1109/pesa.2006.343093)

Raven, N., Schillberg, S., Rasche, S.: Plant cell-based recombinant antibody manufacturing with a 200 L orbitally shaken disposable bioreactor. Methods in Molecular Biology (2016) No. 1385: 161–172 (http://doi.org/10.1007/978-1-4939-3289-4_12)

Schinkel, H., Schillberg, S.: Genome editing: intellectual property and product development in plant biotechnology. Plant Cell Reports (2016) No. 7: 1487-1491(http://doi.org/10.1007/s00299-016-1988-9)

Schmitz, C., Fritsch, L., Fischer, R., Schillberg, S., Rasche, S.: Statistical experimental designs for the production of secondary metabolites in plant cell suspension cultures. Biotechnology letters 38 (2016) No.12: 2007-2014 (http://doi.org/10.1007/s10529-016-2206-0)

Schneider, K., Schiermeyer, A., Dolls, A., Koch, N., Herwartz, D., Kirchhoff, J., Fischer, R., Russell, S.M., Cao, Z., Corbin, D.R., Sastry-Dent, L., Ainley, W.M., Webb, S.R., Schinkel, H., Schillberg, S.: Targeted gene exchange in plant cells mediated by a zinc finger nuclease double cut. Plant Biotechnology Journal 14 (2016) No. 4: 1151-1160 (http://doi.org/10.1111/pbi.12483)

Stolze, A., Wanke, A., van Deenen, N., Geyer, R., Prüfer, R., Schulze Gronover, C.: Development of rubber-enriched dandelion varieties by metabolic engineering of the inulin pathway. Plant Biotechnology Journal (2016) (http://doi.org/10.1111/pbi.12672)

 

V – Z

Vasilev, N., Boccard, J., Lang, G., Grömping, U., Fischer, R., Goepfert, S., Rudaz, S., Schillberg, S.: Structured plant metabolomics for the simultaneous exploration of multiple factors. Scientific Reports (2016) No. 6: Art. 37390 (http://doi.org/10.1038/srep37390)

Vasilev, N., Smales, C.M., Fischer, R., Schillberg S., Schiermeyer, A. (2016): Developments in the production of mucosal antibodies in plants. Biotechnology Advances (2016) No. 34 (2): 77-87 (http://doi.org/10.1016/j.biotechadv.2015.11.002)

Visser, F., Müller, B., Rose, J., Prüfer, D., Noll, G.A.: Forizymes – functionalised artificial forisomes as a platform for the production and immobilisation of single enzymes and multienzyme complexes. Science Reports (2016) No. 6: 30839 (http://doi.org/10.1038/srep30839)

von Felbert, V., Bauerschlag, D., Maass, N., Brautigam, K., Meinhold-Heerlein, I., Woitok, M., et al.: A specific photoimmunotheranostics agent to detect and eliminate skin cancer cells expressing EGFR. Journal of Cancer Research and Clinical Oncology (2016) No. 142(5): 1003-1011 (http://doi.org/10.1007/s00432-016-2122-7)

Woitok, M., Klose, D., Niesen, J., Richter, W., Abbas, M., Stein, C., et al.: The efficient elimination of solid tumor cells by EGFR-specific and HER2-specific scFv-SNAP fusion proteins conjugated to benzylguanine-modified auristatin F. Cancer Letters. (2016) No. 381(2): 323-330 (http://doi.org/10.1016/j.canlet.2016.08.003)