Senior Executive Director

Fraunhofer Institute for Molecular Biology and Applied Ecology IME

Prof. Dr. Rainer Fischer

Prof. Dr. Rainer Fischer received his Ph.D. in Molecular Biology from the Eberhard-Karls Universität Tübingen in 1990. After a postdoctorate with Professor Dr. J. Shively at the Beckman Research Institute of the City of Hope National Medical Center in Duarte (California, USA) he joined Baxter Healthcare Corporation, Immunotherapy Division, Santa Ana (California, USA) as project and group leader. He returned to Germany in 1993 to start his own lab at the RWTH Aachen University.

At present Prof. Dr. Fischer is Professor for Molecular Biotechnology at the RWTH Aachen University and Senior Executive Director of the Fraunhofer Institute for Molecular Biology and Applied Ecology with locations in Aachen, Schmallenberg, Giessen, Münster, Germany. Prof. Fischer's expertise in molecular biotechnology includes the areas of genomics, proteomics, cellomics, protein engineering, molecular medicine, immunology, virology, plant biotechnology as well as the production and purification of recombinant proteins.

As head of the Institute for Molecular Biotechnology at the RWTH Aachen University he supervised more than 77 Bachelor-, 153 Master-thesis and 90 PhD thesis. Prof. Fischer has written 225 peer-reviewed scientific publications, published 65 book chapters. He holds 45 filed and 35 granted patents.

CV

2001 -
present

Full Professor (C4), Institute for Molecular Biotechnology, RWTH Aachen University, Germany  

2001 - present

Senior Executive Director Fraunhofer IME Aachen and Schmallenberg, Germany

1998 - 2001

Group and Division Leader Molecular Biotechnology, Fraunhofer Gesellschaft at the IUCT in Schmallenberg, Germany

1996 - 2001

Senior Assistant Professor at the Institute for Biology I, RWTH Aachen University, Germany

1993 - 1996

Assistant Professor at the Institute for Biology I, RWTH Aachen University, Germany

1991 - 1993

Project and group leader (Baxter Healthcare Corporation, Immunotherapy Division, Santa Ana, California, USA

1990 - 1991

Post doctorate (Prof. Dr. J. Shively at the Beckman Research Institute of the City of Hope National Medical Center in Duarte, California, USA

1987 - 1990

Dissertation project summa cum laude at the University of Tübingen, Germany

1986

MSc in Biology (Eberhard-Karls Universität Tübingen, Germany) Major: Genetics; Minor: Microbiology, Biochemistry and Organic Chemistry

1984 - 1986

Scholar/granted scholarship of the Studienstiftung des deutschen Volkes, Germany

   

Publications

2014

Bialon M, Schellenberg L, Herzog N, Kraus S, Jörißen H, Fischer R, Stein C, Nähring J, Barth S, Püttmann C. (2014) Cloning murine antibody V-genes with non-degenerate primers and conversion to a recombinant antibody format. Monoclonal Antibodies in Immunodiagnosis and Therapy 33(6): 369-377

Boes A, Spiegel H, Edgue G, Kapelski S, Scheuermayer M, Fendel R, Remarque E, Altmann F, Maresch D, Reimann A, Pradel G, Schillberg S, Fischer R (2014) Detailed functional characterization of glycosylated and nonglycosylated variants of malaria vaccine candidate PfAMA1 produced in Nicotiana benthamiana and analysis of growth inhibitory responses in rabbits. Plant Biotechnol J. Sep 18. doi: 10.1111/pbi.12255. [Epub ahead of print]

Bortesi L, Fischer R (2014) The CRISPR/Cas9 system for plant genome editing and beyond. Biotechnol Adv. 2014 Dec 20. pii: S0734-9750(14)00193-1. doi: 10.1016/j.biotechadv.2014.12.006. [Epub ahead of print]

Brehm H, Niesen J, Mladenov R, Stein C, Pardo A, Fey G, Helfrich W, Fischer R, Gattenlöhner S, Tur MK, Barth S (2014) A CSPG4-specific immunotoxin kills rhabdomyosarcoma cells and binds to primary tumor tissue. Cancer Lett 352(2) 228–235

Buyel JF, Fischer R (2014) Synthetic polymers are more effective than natural flocculants for the clarification of tobacco leaf extracts. J Biotechnol. doi: 10.1016/j.jbiotec.2014.12.018.

Buyel JF, Fischer R (2014) Scale-down models to optimize a filter train for the downstream purification of recombinant pharmaceutical proteins produced in tobacco leaves. Biotechnology Journal, 9: 415–425. doi: 10.1002/biot.201300369

Buyel JF, Fischer R (2014) Downstream processing of biopharmaceutical proteins produced in plants: the pros and cons of flocculants. Bioengineered. 5(2):138-42. doi: 10.4161/bioe.28061.

Buyel JF, Fischer R (2014) Characterization of complex systems using the design of experiments approach: transient protein expression in tobacco as a case study. J Vis Exp. (83):e51216. doi: 10.3791/51216.

Buyel JF, Fischer R (2014) Generic chromatography-based purification strategies accelerate the development of downstream processes for biopharmaceutical proteins produced in plants. Biotechnol J. 9(4):566-77. doi: 10.1002/biot.201300548.

Buyel JF, Fischer R (2014) Flocculation increases the efficacy of depth filtration during the downstream processing of recombinant pharmaceutical proteins produced in tobacco. Plant Biotechnol J. 2014 Feb;12(2):240-52. doi: 10.1111/pbi.12132.

Buyel JF, Buyel JJ, Haase C, Fischer R (2014) The impact of Pseudomonas syringae type III effectors on transient protein expression in tobacco.Plant Biol doi: 10.1111/plb.12264. [Epub ahead of print]

Dehghan S, Sadeghi M, Pöppel A, Fischer R, Lakes-Harlan R, Kavousi HR, Vilcinskas A, Rahnamaeian M (2014) Differential inductions of phenylalanine ammonia-lyase and chalcone synthase during wounding, salicylic acid treatment, and salinity stress in safflower, Carthamus tinctorius. Biosci Rep. 34(3). pii: e00114. doi: 10.1042/BSR20140026.

Dickmeis C, Fischer R, Commandeur U (2014) Potato virus X-based expression vectors are stabilized for long-term production of proteins and larger inserts. Biotechnol J. doi: 10.1002/biot.201400347.

Garvey M, Klinger J, Klose H, Fischer R, Commandeur U (2014) Expression of recombinant cellulase Cel5A from Trichoderma reesei in tobacco plants. J Vis Exp (88). doi: 10.3791/51711

Hristodorov D, Amoury M, Mladenov R, Niesen J, Arens K, Berges N, Hein L, Di Fiore S, Pham AT, Huhn M, Helfrich W, Fischer R, Thepen T, Barth S (2014) EpCAM-selective elimination of carcinoma cells by a novel MAP-based cytolytic fusion protein. Mol Cancer Ther 3(9) 2194-2202

Hristodorov D, Mladenov R, Brehm H, Fischer R, Barth S, Thepen T (2014) Recombinant H22(scFv) blocks CD64 and prevents the capture of anti-TNF monoclonal antibodies - a potential strategy to enhance anti-TNF-alpha therapy. MAbs 6(5): 1283-1289

Hristodorov D, Nordlohne J, Mladenov R, Schiffer S, Huhn M, Fischer R, Thepen T, Barth S (2014) Human microtubule-associated protein tau mediates targeted killing of CD30(+) lymphoma cells in vitro and inhibits tumour growth in vivo. Brit J Haematol 164(2): 251-257

Honke N, Ohl K, Wiener A, Bierwagen J, Peitz J, Di Fiore S, Fischer R, Wagner N, Wüller S, Tenbrock K (2014) The p38-mediated rapid down-regulation of cell surface gp130 expression impairs interleukin-6 signaling in the synovial fluid of juvenile idiopathic arthritis patients. Arthritis Rheumatol. 66(2):470-8. doi: 10.1002/art.38245.

Jansen F, Gillessen B, Mueller F, Commandeur U, Fischer R, Kreuzaler F (2014) Metabolic engineering for p-coumaryl alcohol production in Escherichia coli by introducing an artificial phenylpropanoid pathway. Biotechnol Appl Biochem. 61(6):646-54. doi: 10.1002/bab.1222

Kapelski S, Klockenbring T, Fischer R, Barth S, Fendel R (2014) Assessment of the neutrophilic antibody-dependent respiratory burst (ADRB) response to Plasmodium falciparum. J Leukoc Biol. 2014 Dec;96(6):1131-42. doi: 10.1189/jlb.4A0614-283RR.

Kern S, Agarwal S, Huber K, Gehring AP, Strödke B, Wirth CC, Brügl T, Abodo LO, Dandekar T, Doerig C, Fischer R, Tobin AB, Alam MM, Bracher F, Pradel G. (2014) Inhibition of the SR protein-phosphorylating CLK kinases of Plasmodium falciparum impairs blood stage replication and malaria transmission. PLoS One. 4;9(9):e105732. doi: 10.1371

Kovačević G, Blažić M, Draganić B, Ostafe R, Gavrović-Jankulović M, Fischer R, Prodanović R. (2014) Cloning, heterologous expression, purification and characterization of M12 mutant of Aspergillus niger glucose oxidase in yeast Pichia pastoris KM71H. Mol Biotechnol. 56(4):305-11. doi: 10.1007/s12033-013-9709-x.

Lambertz C, Garvey M, Klinger J, Heesel D, Klose H, Fischer R, Commandeur U (2014) Challenges and advances in the heterologous expression of cellulolytic enzymes: a review. Biotechnol Biofuels 7(1):135. doi: 10.1186/s13068-014-0135-5

Lee KL, Uhde-Holzem K, Fischer R, Commandeur U, Steinmetz NF. (2014) Genetic engineering and chemical conjugation of potato virus x. Methods Mol Biol1108:3-21.

Jansen F, Gillessen B, Mueller F, Commandeur U, Fischer R, Kreuzaler F. (2014) Metabolic engineering for p-coumaryl alcohol production in Escherichia coli by introducing an artificial phenylpropanoid pathway. Biotechnol Appl Biochem. 61(6):646-54.

Mandal MK, Fischer R, Schillberg S, Schiermeyer A (2014) Inhibition of protease activity by antisense RNA improves recombinant protein production in Nicotiana tabacum cv. Bright Yellow 2 (BY-2) suspension cells. Biotechnol J. 9(8):1065-73. doi: 10.1002/biot.201300424.

Niesen J, Brehm H, Stein C, Berges N, Pardo A, Fischer R, ten Haaf A, Gattenlöhner S, Tur MK, Barth S (2014) In vitro effects and ex vivo binding of an EGFR-specific immunotoxin on rhabdomysarcoma cells. J Cancer Res Clin Oncol DOI 10.1007/s00432-014-1884-z

Ostafe R, Prodanovic R, Nazor J, Fischer R. (2014) Ultra-high-throughput screening method for the directed evolution of glucose oxidase. Chem Biol. 21(3):414-21. doi: 10.1016/j.chembiol.2014.01.010.

Ostafe R, Prodanovic R, Lloyd Ung W, Weitz DA, Fischer R (2014) A high-throughput cellulase screening system based on droplet microfluidics. Biomicrofluidics 8(4):041102. doi: 10.1063/1.4886771.

Popovic M, Prodanovic R, Ostafe R, Schillberg S, Fischer R, Gavrovic-Jankulovic M (2014) Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens. Immunol Res. 2014 Dec 24. [Epub ahead of print]

Raven N, Rasche S, Kuehn C, Anderlei T, Klöckner W, Schuster F, Henquet M, Bosch D, Büchs J, Fischer R, Schillberg S (2014) Scaled-up manufacturing of recombinantantibodies produced by plant cells in a 200-L orbitally-shaken disposable bioreactor. Biotechnol Bioeng. 2014 Aug 13. doi: 10.1002/bit.25352. [Epub aheadof print]

Salgado D, Fischer R, Schillberg S, Twyman RM, Rasche S (2014) Comparative evaluation of heterologous production systems for recombinant pulmonary surfactant protein d. Front Immunol. doi: 10.3389/fimmu.2014.00623.

Schiller V, Zhang X, Hecker M, Schäfers C, Fischer R, Fenske M (2014) Species-specific considerations in using the fish embryo test as an alternative to identify endocrine disruption. Aquat Toxicol. 155:62-72.

Schramm OG, López-Cortés X, Santos LS, Laurie VF, González Nilo FD, Krolik M, Fischer R, Di Fiore S (2014) pH-dependent nano-capturing of tartaric acid using dendrimers. Soft Matter 10(4):600-8. doi: 10.1039/c3sm52255e.

Sommer J, Garbers C, Wolf J, Trad A, Moll JM, Sack M, Fischer R, Grötzinger J, Waetzig GH, Floss DM, Scheller J (2014) Alternative intronic polyadenylation generates the interleukin-6 trans-signaling inhibitor sgp130-E10. J Biol Chem. 289(32):22140-50. doi: 10.1074/jbc.M114.560938

Spiegel H, Schinkel H, Kastilan R, Dahm P, Boes A, Scheuermayer M, Chudobová I, Maskus D, Fendel R, Schillberg S, Reimann A, Fischer R (2014) Optimization of a multi-stage, multi-subunit malaria vaccine candidate for the production in Pichia pastoris by the identification and removal of protease cleavage sites. Biotechnol Bioeng. 2014 Oct 21. doi: 10.1002/bit.25481. [Epub ahead of print]

Stoger E, Fischer R, Moloney M, Ma JK (2014) Plant molecular pharming for the treatment of chronic and infectious diseases. Annu Rev Plant Biol. 65:743-68. doi: 10.1146/annurev-arplant-050213-035850.

Vasilev N, Schmitz C, Grömping U, Fischer R, Schillberg S (2014) Assessment of cultivation factors that affect biomass and geraniol production in transgenic tobacco cell suspension cultures. PLoS One. 9(8):e104620. doi:10.1371

Voepel N, Boes A, Edgue G, Beiss V, Kapelski S, Reimann A, Schillberg S, Pradel G, Fendel R, Scheuermayer M, Spiegel H, Fischer R (2014) Malaria vaccine candidate antigen targeting the pre-erythrocytic stage of Plasmodium falciparum produced at high level in plants. Biotechnol J. 9(11):1435-45. doi: 10.1002/biot.201400350

2013

Blazic M, Kovacevic G, Prodanovic O, Ostafe R, Gavrovic-Jankulovic M, Fischer R, Prodanovic R. (2013) Yeast surface display for the expression, purification and characterization of wild-type and B11 mutant glucose oxidases. Protein Expr Purif. 89(2):175-80. doi:10.1016/

Buyel JF, Fischer R (2013) Processing heterogeneous biomass: overcoming the hurdlesin model building. Bioengineered. 4(1):21-4. doi: 10.4161/bioe.21671.

Buyel JF, Kaever T, Buyel JJ, FischerR (2013) Predictive models for the accumulation of a fluorescent marker protein in tobacco leaves according to the promoter/5'UTR combination. Biotechnology and Bioengineering 110: 471-482.

Buyel JF, Woo JA, Cramer, SM Fischer R (2013) The use of quantitative structure-activity relationship models to develop optimized processes for the removal of tobacco host cell proteins during biopharmaceutical production. J. Chromatogr.A 1322, 18-28.

Díaz C, Molina AM, Nähring J, Fischer R (2013) Characterization and dynamic behavior of wild yeast during spontaneous wine fermentation in steel tanks and amphorae. Journal of Biomedicine and Biotechnology (BioMed Research International), ID 540465. DOI:10.1155/2013/540465.

Feller T, Thom P, Koch N, Spiegel H, Addai-Mensah O, Fischer R, Reimann A, Pradel G, Fendel R, Schillberg S, Scheuermayer M, Schinkel H (2013) Plant-based production of recombinant Plasmodium surface protein pf38 and evaluation of ist potential as a vaccine candidate. PLoS One. 8(11):e79920. doi: 10.1371/journal.pone.0079920.

Fischer R, Schillberg S, Buyel JF, Twyman RM (2013) Commercial aspects of pharmaceutical protein production in plants. Curr Pharm Des. 19(31):5471-7.

Fischer R, Ostafe R, Twyman RM (2013) Cellulases from insects. Adv Biochem Eng Biotechnol. 2013;136:51-64. doi: 10.1007/10_2013_206.

Garvey M, Klose H, Fischer R, Lambertz C, Commandeur U (2013) Cellulases for biomass degradation: comparing recombinant cellulase expression platforms. Trends Biotechnol. 31(10):581-93. doi: 10.1016/j.tibtech.2013.06.006.

Hristodorov D, Fischer R, Joerissen H, Müller-Tiemann B, Apeler H, Linden L (2013) Generation and comparative characterization of glycosylated and aglycosylated human IgG1 antibodies. Mol Biotechnol. 53(3):326-35. doi: 10.1007/s12033-012-9531-x.

Hristodorov D, Fischer R, Linden L (2013) With or without sugar? (A)glycosylation of therapeutic antibodies. Mol Biotechnol. 54(3):1056-68. doi: 10.1007/s12033-012-9612-x.

Klose H, Günl M, Usadel B, Fischer R, Commandeur U. (2013) Ethanol inducible expression of a mesophilic cellulase avoids adverse effects on plant development. Biotechnol Biofuels, 6:53.

Mukherjee K, Hain T, Fischer R, Chakraborty T, Vilcinskas A (2013) Brain infection and activation of neuronal repair mechanisms by the human pathogen Listeria monocytogenes in the lepidopteran model host Galleria mellonella. Virulence 4: 1-9.

Mukherjee K, Raju R, Fischer R, Vilcinskas A (2013) Galleria mellonella as a model host to study gut microbe homeostasis and brain infection by the human pathogen Listeria monocytogenes. Adv Biochem Eng Biotechnol. DOI: 10.1007/10_2013_203.

Ngwa CJ, Glöckner V, Ramadan Abdelmohsen U, Scheuermeyer M, Fischer R, Hentschel U, Pradel G (2013) 16S rRNA gene-based identification of Elizabethkingia meningoseptica (Flavobacteriales: Flavobacteriaceae) as a dominant midgut bacterium of the asian malaria vector Anopehles stephensi (Dipteria: Culicidae) with antimicrobial activities. Journal of Medical Entomology 50 (2): 404-414.

Ngwa CJ, Scheuermayer M, Mair GR, Kern S, Brügl T, Wirth CC, Aminake MN, Wiesner J, Fischer R, Vilcinskas A, Pradel G (2013) Changes in the transcriptome of the malaria parasite Plasmodium falciparum during the initial phase of transmission from the human to the mosquito. BMC Genomics. 14:256. doi:10.1186/1471-2164-14-256.

Ostafe R, Prodanovic R, Commandeur U, Fischer R (2013) Flow cytometry-based ultra-high-throughput screening assay for cellulase activity. Anal Biochem. 435(1):93-8.

Puettmann C, Kolberg K, Hagen S, Schmies S, Fischer R, Naehring J, Barth S (2013) A monoclonal antibody for the detection of SNAP/CLIP-tagged proteins. Immunol Lett. 150(1-2):69-74. doi: 10.1016/j.imlet.2012.10.007.

Rosenberg Y, Sack M, Montefiori D, Forthal D, Mao L, Hernandez-Abanto S, Urban L, Landucci G, Fischer R, Jiang X (2013) Rapid high-level production of functional HIV broadly neutralizing monoclonal antibodies in transient plant expression systems. PLoS One. 8(3):e58724. doi: 10.1371/journal.pone.0058724.

Pradel G, Simon N, Lasonder E, Scheuermayer M, Kühn A, Fischer R, Zipfel P, Skerka G (2013) The impact of complement evasion on transmission of malaria. Molecular Immunology, Vol 56, pp 243.

Puettmann C, Kolberg K, Hagen S, Schmies S, Fischer R, Naehring N, Barth S (2013) A monoclonal antibody for the detection of SNAP/CLIP-tagged proteins. Immunology Letters 150: 69-74.

Sadeghi M, Dehghan S, Fischer R, Wenzel U, Vilcinskas A, Kavousi HR, Rahnamaeian M (2013) Isolation and characterization of isochorismate synthase and cinnamate 4-hydroxylase during salinity stress, wounding, and salicylic acid treatment in Carthamus tinctorius. Plant Signal Behav. 8(11):e27335. doi: 10.4161/psb.27335.

Schiffer S, Hansen HP, Hehmann-Titt GM, Huhn M, Fischer R, Barth S, Thepen T (2013) Efficacy of an adapted granzyme B-based anti-CD30 cytolytic fusion protein against PI-9-positive classical Hodgkin lymphoma cells in a murine model. Blood Cancer Journal 3, e106; doi:10.1038/bcj.2013.4.

Schiffer S, Hristodorov D, Mladenov R, Aslanian E, Huhn M, Fischer R, Barth S, Thepen T (2013) Species-dependent functionality of the human cytolytic fusion proteins Granzyme B-H22(scFv) and H22(scFv)-Angiogenin in macrophages. Antibodies 2, 9-18.

Schiffer S, Letzian S, Jost E, Mladenov R, Hristodorov D, Huhn M, Fischer R, Barth S, Thepen T (2013) Granzyme M as a novel effector molecule for human cytolytic fusion proteins: CD64-specific cytotoxicity of Gm-H22(scFv) against leukemic cells. Cancer Lett. 341(2):178-85. doi: 10.1016/j.canlet.2013.08.005.

Schillberg S, Raven N, Fischer R, Twyman RM, Schiermeyer A (2013) Molecular farming of pharmaceutical proteins using plant suspension cell and tissue cultures. Curr Pharm Des. 19(31):5531-42

Schiller V, Wichmann A, Kriehuber R, Schäfers C, Fischer R, Fenske M (2013) Transcriptome alterations in zebrafish embryos after exposure to environmental estrogens and anti-androgens can reveal endocrine disruption. Reprod Toxicol. 42:210-23. doi: 10.1016/j.reprotox.2013.09.003.

Simon N, Lasonder E, Scheuermayer M, Kuehn A, Fischer R, Zipfel PF, Skerka C, Pradel G (2013) Malaria parasites co-opt human factor H to protect from complement mediated lysis in the mosquito midgut. Cell Host Microbe 13: 29-41.

Twyman RM, Schillberg S, Fischer R (2013) Optimizing the yield of recombinant pharmaceutical proteins in plants. Curr Pharm Des. 19(31):5486-94.

Vasilev N, Grömping U, Lipperts A, Raven N, Fischer R, Schillberg S (2013) Optimization of BY-2 cell suspension culture medium for the production of a human antibody using a combination of fractional factorial designs and the response surface method. Plant Biotechnol J. 11(7):867-74. doi: 10.1111/pbi.12079.

Viell J, Wulfhorst H, Schmidt T, Commandeur U, Fischer R, Spiess A, Marquardt W (2013) An efficient process for the saccharification of wood chips by combined ionic liquid pretreatment and enzymatic hydrolysis. Bioresour Technol. 146:144-51. doi: 10.1016/j.biortech.2013.07.059.

2012

Buyel JF, Bautista JA, Fischer R, Yusibov VM (2012) Extraction, purification and characterization of the plant-produced HPV16 subunit vaccine candidate E7 GGG. J. Chromatogr. B 880: 19-26.

Buyel JF, Fischer R (2012) Predictive models for transient protein expression in tobacco (Nicotiana tabacum L.) can optimize process time, yield and downstream costs. Biotechnol. Bioeng.109: 2575-2588.

Buyel JF, Fischer R (2012) Processing heterogeneous biomass. Bioengineered, 4: 1-4.

Fischer R, Schillberg S, Hellwig S, Twyman RM, Drossard J (2012) GMP issues for recombinant plant-derived pharmaceutical proteins. Biotechnol. Adv. 30: 434-439.

Harig L, Beinecke FA, Oltmanns J, Muth J, Müller O, Rüping B, Twyman RM, Fischer R, Prüfer D, Noll GA (2012) Proteins from the FLOWERING LOCUS T-like subclade of the PEBP family act antagonistically to regulate floral initiation in tobacco. Plant J. 72(6):908-21. doi: 10.1111/j.1365-313X.2012.05125.x.

Hristodorov D, Fischer R, Joerissen H, Müller-Tiemann B, Apeler H, Kubdeb K (2012) Generation and comparative characterization of glycosylated and aglycolsylated human IgG1 Antibodies. Mol Biotech 25: 1-13.

Kirchhoff J, Raven N, Boes A, Roberts JL, Russel S, Treffenfeldt W, Fischer R, Schinkel H, Schiermeyer A, Schillberg S (2012) Monoclonal tobacco cell lines with enhanced recombinant protein yields can be generated from heterogeneous cell suspension cultures by flow sorting. Plant Biotechnol J 10: 936-944.

Klose H, Röder J, Girfoglio M, Fischer R, Commandeur U (2012) Hpyerthermophilic endoglucanase for in planta lignocellulose conversion. Biotechnol Biofuels 5: 63. 

Mukherjee K, Fischer R, Vilcinskas A (2012) Histone acetylation mediates epigenetic regulation of transcriptional reprogramming in insects during metamorphosis, wounding and infection. Frontiers in Zoology 9: 1.

Pardo A, Stöcker M, Kampmeier F, Melmer G, Fischer R, Thepen T, Barth S (2012) In vivo imaging of immunotoxin treatment using Katushka-transfected A-431 cells in a murine xenograft tumour model. Cancer Immunol Immunother 61: 1617-1626.

Röhrich CR, Ngwa CJ, Wiesner J, Schmidtberg H, Degenkolb T, Kollewe C, Fischer R, Pradel G, Vilcinskas A (2012) Harmonine, a defence compound from the harlequin ladybird, inhibits mycobacterial growth and demonstrates multi-stage antimalarial activity. Biol Lett. 8(2):308-11. doi: 10.1098/rsbl.2011.0760.

Wahler D, Colby T, Kowalski NA, Harzen A, Wotzka SY, Hillebrand A, Fischer R, Helsper J, Schmidt J, Schulze Gronover C, Prüfer D (2012) Proteomic analysis of latex from the rubber-producing plant Taraxacum brevicorniculatum. Proteomics 12: 901-905.

Wu Z, Chen K, Yildiz I, Dirksen A, Fische R, Dawson PE, Steinmetz NF (2012) Development of viral nanoparticles for efficient intracellular delivery. Nanoscale 4: 3698-3705.

Zakri AM, Ziegler A, Commandeur U, Fischer R, Torrance L (2012) In vivo expression and binding activity of scFv-RWAV, which recognizes the coat protein of tomato leaf curl New Delhi virus (family Geminiviridae). Arch Virol 157: 1291-1299.

2011

Boes A, Spiegel H, Delbrück H, Fischer R, Schillberg S, Sack M (2011) Affinity purification of a framework 1 engineered mouse/human chimeric IgA2 antibody from tobacco. Biotechnol. Bioeng. 108: 2804-2814.

Fischer R (2011) Purification methods in biomanufacturing. Genetic Engineering & Biotechnology News 31: 56-58.

Jäger G, Girfoglio M, Dollo F, Rinaldi R, Bongard H, Commandeur U, Fischer R, Spiess AC, Büchs J. (2011) How recombinant swollenin from Kluyveromyces lactis affects cellulosic substrates and accelerates their hydrolysis. Biotechnol Biofuels. 4(1):33. doi: 10.1186/1754-6834-4-33.

Rasche S, Martin A, Holzem A, Fischer R, Schinkel H, Schillberg S (2011) One-step protein purification: Use of a novel epitope tag for highly efficient detection and purification of recombinant proteins. The open Biotechnology Journal 5: 1-6.

Röhrich CR, Ngwa CJ, Wiesner J, Schmidtberg H, Degenkolb T, Kollewe C, Fischer R, Pradel G, Vilcinskas A (2011) Harmonine, a defence compound from the harlequin ladybird, inhibits mycobacterial growth and demonstrates multi-stage antimalarial activity. Biology Letters 8: 308-311.

Stoger S, Rademacher T, Arcalis E, Sack M, Stiegler G, Altmann F, Fischer R (2011) From recombinant proteins to plant-made-pharmaceuticals. Current Opinion in Biotechnology 22: 17.

Taghavian O, Mandal MK, Steinmetz NF, Rasche S, Spiegel H, Fischer R, Schillberg S (2011) A potential nanobiotechnology platform based on infectious bursal disease subviral particles. RSC Advances 2: 1970-1978.

2010

Arcalis E, Stadlmann J, Sylvain M, Drakakai G, Winter V, Rodriguez J, Fischer R, Altmann F, Stoger E (2010) The changing fate of a secretory glycoprotein in developing maize endosperm. Plant Phys 153:693-702.

Holland T, Sack M, Rademacher T, Schmale K, Altmann F, Stadlmann J, Fischer R, Hellwig S (2010): Optimal nitrogen supply as a key to increased and sustained production of a monoclonal full-size antibody in BY-2 suspension culture. Biotech Bioeng107 (2): 278-289.

Kampmeier F, Niesen J, Koers A, Ribbert M, Brecht A, Fischer R, Kießling F, Barth S, Thepen T (2010): Rapid optical imaging of EGF-receptor expression with a single chain antibody SNAP-tag fusion protein. European Journal of Nuclear Medicine and Molecular Imaging, 37:1926-1934.

Kowollik S, Schnitzler M, Biselli M, Krueger R, Zang W, Peuscher S, Schillberg S, Fischer R (2010) Die Rolle des Respirationsquotienten in der Zellkulturfermentation. Chemie Ingenieur Technik 82 (9): 1505-1506.

Mandal MK, Fischer R, Schillberg S, Schiermeyer A (2010): Biochemical properties of the matrix-metalloproteinase NtMMP1 from Nicotiana tabacum cv. BY-2 suspension cells. Planta 232(4):899-910.

Müsken M, Di Fiore S, Dötsch A, Fischer R, Häussler S (2010): Genetic determinants of Pseudomonas aeruginosa biofilm development. Microbiology 76(1):310-317

Ribbert T, Thepen T, Tur MK, Fischer R, Huhn M, Barth S (2010): Recombinant, ETA’-based CD64 immunotoxins: improved efficacy by increased valency, both in vitro and in vivo in a chronic cutaneous inflammation model in human CD64 transgenic mice. Br J Dermatol 163(2): 279-286.

Sainsbury F, Sack M, Stadlmann J, Quendler H, Fischer R, Lomonossoff GP (2010): Rapid Transient Production in Plants by Replicating and Non-Replicating Vectors Yields High Quality Functional Anti-HIV Antibody. PLoS one 5(11): e13976.

Schmidt T, Hillebrand A, Wurbs D, Wahler D, Lenders M, Fischer R, Schulze Gronover C, Prüfer D (2010): Molecular cloning and characterization of rubber biosynthetic genes from Taraxacum koksaghyz. Plant Mol Biol Rep 28(2):277-2584.

Schubert Max, Agdour S, Fischer R, Olbrich Y, Schinkel H, Schillberg S (2010): A Monoclonal Antibody that specifically binds chitosan in vitro and in Situ on fungal cell walls. J. Microbiol. Biotechnol. 20(8): 1179-1184.

Uhde-Holzem K, Fischer R, Commandeur U (2010): Characterization and diagnostic potential of foreign epitope-presenting Ty1 virus-like particles expressed in Escherichia coli and Pichia pastoris. J Mol Microbio Biotech 18(1):52-62.

Uhde-Holzem K, Schlösser V, Vaizov S, Fischer R, Commandeur U (2010) Immunogenic properties of chimeric potato virus X particles displaying the hepatitis C virus hypervariable region I peptide R. Journal of Virological Methods 166: 12-20.

vom Stein T, Grande P, Sibilla F, Commandeur U, Fischer R, Leitner W, Dominguez de Maria P (2010) Salt-assisted organic-acid-catalyzed depolymerization of cellulose. Green Chemistry 12: 1844-1849.

Zakri AM, Ziegler A, Torrance L, Fischer R, Commandeur U (2010): Generation and characterization of a scFv against recombinant coat protein of the geminivirus tomato leaf curl New Delhi virus. Arch Virol 155(3): 335-342.

2009

Bebrone C, Delbrueck H, Kupper MB, Schlomer P, Willmann C, Frere JM, Fischer R, Gelleni M, Hoffmann KMV (2009) The structure of the dizinc subclass B2 metallo-beta-lactamase CphA reveals that the second inhibitory zinc ion binds in the histidine site. Antimicrob Agents Ch 53: 4464-4471.

Cogotzi L, Giampetruzzi A, Nölke G, Orecchia M, Elicio V, Castellano MA, Martelli G, Fischer R, Schillberg S, Saldarelli P (2009) An assay for the detection of Grapevine leafroll-associated virus 3 using a single-chain fragment variable antibody. Arch Virol 154: 19-26.

Floss DM, Sack M, Arcalis E, Stadlmann J, Quendler H, Rademacher T, Stoger E, Scheller J, Fischer R, Condrad U (2009) Influence of elastin-like peptide fusions on the quantity and quality of a tobacco-derived human immunodeficiency virus-neutralizing antibody. Plant Biol 7: 899-913.

Lipinski M, Scholz M, Pieper K, Fischer R, Prüfer D, Müller KJ (2009) A squalene epoxidase from Nigella sativa participates in saponin biosynthesis and mediates terbinafine resistance in yeast. Cent Eur J Biol 4: 163-169.

Loesing JB, Di Fiore S, Ritter K, Fischer R, Kleines M (2009) The BDLF2/BMRF2 complex of EBV affects cellular morphology. Journal Gen Virol 90: 1440-1449.

Nachreiner T, Kampmeier F, Thepen T, Fischer R, Barth S, Stöcker M (2009) Depletion of autoreactive B-lymphocytes by a recombinant myelin oligodendrocyte glycoprotein-based immunotoxin. J Neuroimmunol. 195: 28-35.

Noelke G, Cobanov P, Uhde-Holzem K, Reustle G, Fischer R, Schillberg S (2009) Grapevine fanleaf (GFLV)-specific antibodies confer GFLV and Arabis mosaic virus (ArMV) resistance in Nicotiana benthamiana. Mol Plant Pathol. 10: 41-49.

Noll GA, Rüping B, Ernst AM, Bucsenez M, Twyman RM, Fischer R, Prüfer D (2009) The promoters of forisome genes MtSE02 and MtSE03 direct gene expression to immature sieve elements in Medicago truncatula and Nicotiana tabacum. Plant Mol Biol Rep. 27: 526-533.

Ranft K, Thepen T, Fischer R, Barth S, Stöcker M (2009) Recombinant bispecific single chain antibody fragments induce Fc-gamma receptor-mediated elimination of DC30+ lymphoma cells. Cancer Lett. 282: 187-194.

Scholz M, Lipinski M, Leupold M, Luftmann H, Harig L, Ofir R, Fischer R, Prüfer D, Müller KJ (2009) Methyl jasmonate induced accumulation of kalopanaxsaponin I in Nigella sativa. Phytochem. 70: 517-522.

Sorrentino A, Schillberg S, Fischer R, Porta R, Mariniello L (2009) Molecular farming of human tissue transglutaminase in tobacco plants. Amino Acids 36: 765-772.

Steinmetz NF, Mertens M, Taurog R, Johnson J, CommandeurU, Fischer R, Manchester M (2009) Potato virus X as a novel platform for potential biomedical applications. Nano Lett. 10: 305-312.

Wahler D, Schulze Gronover C, Richter C, Foucu F, Twyman RM, Moerschbacher BM, Fischer R, Prüfer D (2009) Polyphenooxidase silencing affects latex coagulation in Taraxacum spp. Plant Phys. 151: 334-346.

2008

Chung WY, Sack M, Carter R, Spiegel, H, Fischer R, Hirst TR, Williams NA, James RF (2008) Phage-display derived single-chain fragment variable (scFv) antibodies recognizing conformational epitopes of Escherichia coli heat-labile enterotoxin B-subunit. J Immunol Meth 339: 115-123.

Hetzel C, Bachran C, Fischer R, Fuchs H, Barth S, Stöcker M (2008) Small cleavable adapters enhance the specific cytotoxicity of a humanized immunotoxin directed against CD64 positive cells. Internat J Cancer 31: 370-376.

Floss D, Sack M, Rademacher T, Fischer R, Conrad U (2008) Biochemical and functional characterization of anti-HIV antibody ELP fusion proteins from transgenic plants. Plant Biotech J 6: 379-391.

Stahnke B, Thepen T, Stöcker M, Rosinke R, Jost E, Fischer R, Tur MK, Barth S (2008) Granzyme B-H22 (scFv), a human immunotoxin targeting CD64 in acute myeloid leukaemia of monocytic subtypes. Mol Cancer Ther 7: 2924-2932.

Daghan H, Schaeffer A, Fischer R, Commandeur U (2008) Phytoextraction of cadmium from contaminated soil using transgenenic tobacco plants. J. Int. Environ. Appl. Sci. 3: 336-345.

Li HP, Zhang JB, Shi RP, Huang T, Fischer R, Liao YC (2008) Engineering fusarium head blight resistance in wheat by expression of a fusion protein containing a Fusarium-specific antibody and an antifungal peptide. Mol Plant Microbe Interact 21: 1242-1248.

Zhang MY, Zimmermann S, Fischer R, Schillberg S (2008) Generation and evaluation of movement-protein-specific single-chain antibodies for delaying symptoms of Tomato spotted wilt virus infection in tobacco. Plant Pathol 57: 854-860.

Spök A, Twyman RM, Fischer R, Ma JKC, Sparrow PAC (2008) Evolution of a regulatory framework for pharmaceuticals derived from genetically modified plants. Trends Biotechnol 26: 506-517.

Nölke G, Schneider B, Agdour S, Drossard J, Fischer R, Schillberg S (2008) Modulation of polyamine biosynthesis in transformed tobacco plants by targeting ornithine decarboxylase to an atypical subcellular compartment. Open Biotech J 2: 183-189.

Nickel H, Kawchuk L, Twyman RM, Zimmermann S, Junghans H, Winter S, Fischer R, Prüfer D (2008) Plantibody-mediated inhibition of the Potato leafroll virus P1 protein reduces virus accumulation. Virus Res 136: 140-145.

Orecchia M, Nölke G, Saldarelli P, Dell’Orco M, Uhde-Holzem K, Sack M, Martelli G, Fischer R, Schillberg S (2008) Generation and characterization of a recombinant antibody fragment that binds to the coat protein of Grapevine leafroll associated virus 3. Arch Virol 153: 1075-1084.

Stöcker M, Pardo A, Hetzel C, Reutelingsberger C, Fischer R, Barth S (2008) Eukaryotic expression and secretion of EGFP-labeled annexin A5. Protein Expr Purif 58: 325-331.

Rademacher T, Sack M, Arcalis E, Stadlmann J, Balzer S, Altmann F, Quendler H, Stiegler G, Kunert R, Fischer R, Stoger E (2008) Recombinant antibody 2G12 produced in maize endosperm efficiently neutralizes HIV-1 and contains predominantly single-GlcNAc N-glycans. Plant Biotech J 6: 189-201.

2007

Maclean J, Koekemoer M, Olivier AJ, Stewart D, Hitzeroth II, Rademacher T, Fischer R, Williamson AL, Rybicki EP (2007) Optimization of human papillomavirus type (HPV-16) L1 expression in plants: comparison of the suitability of different HPV-16 L1 gene variants and different cell-compartment localization. J Gen Virol 88: 1460-1469.

Ribbert M, Wolters A, Barth S, Stöcker M, Schaeffer A, Fischer R, Finnern R (2007) Immunodetection of venturia inaequalis ascospores with phage antibodies. J Phytopathol 155: 170-177.

Noll GA, Fontanellaz ME, Rüping B, Ashoub A, van Bel AJE, Fischer R, Knoblauch M, Prüfer D (2007) Spatial and temporal regulation of the forisome gene for1 in the phloem during plant development Plant Mol Biol 65: 285-294.

Segers K, Sperandio O, Sack M, Fischer M, Miteva MA, Rosing J, Nicolaes GAF, Villoutreix BO (2007) Design of protein-membrane interaction inhibitors by virtual ligand screening, proof of concept with the C2 domain of factor V. Proc Natl Acad Sci USA 104: 12697-12702.

Ruthardt N, Kawchuk LM, Fischer R, Emans N (2007) Tomato protein of the resistance gene Ve2 to verticillium wilt [Verticillium spp.] is located in the endoplasmic reticulum. Can J Plant Pathol 29: 3-8.

Safarnejad MR, Fischer R, Commandeur U (2007) Generation and characterization of functional recombinant antibody fragments against tomato yellow leaf curl virus replication-associated protein. Commun Agric Appl Biol Sci 73: 311-321.

Uhde-Holzem K, Fischer R, Commandeur U (2007) Genetic stability of recombinant potato virus X virus vectors presenting foreign epitopes. Arch Virol 152: 805-811.

2006

Drakakaki G, Marcel S, Arcalis E, Altmann F, Gonzalez-Melendi P, Fischer R, Christou P, Stoger E (2006) The intracellular fate of a recombinant protein is tissue dependent. Plant Phys 141: 578-586.

Lan P, Li W, Fischer R (2006) Arabidopsis thaliana wild type, pho1, and pho2 mutant plants show different responses to exogenous cytokinins. Plant Phys Biochem 44: 343-350.

Müller KJ, He X, Fischer R, Prüfer D (2006) Constitutive knox1 gene expression in dandelion (Taraxacum officinale, Web.) changes leaf morphology from simple to compound. Planta 224: 1023-1027.

Müller KJ, Lin J, Fischer R, Prüfer D (2006) Repeated epiphylly correlates with resident knox1 gene expression in leaves of tobacco epiphyllous shoots. Cent Eur J Biol 1: 263-274.

Nölke G, Fischer R, Schillberg S (2006) Antibody-based metabolic engineering in plants. J Biotech 124: 271-283.

Piepenbreier K, Renn J, Fischer R. and Goerlich R. (2006): Influence of space flight conditions on phenotypes and function of nephritic immune cells of swordtail fish (Xiphophorus helleri). Adv. Space Res 38 (6): 1016-1024.

Renn J, Winkler C, Schartl M, Fischer R, Goerlich R (2006) Zebrafish and medaka as models for bone research including implications regarding space-related issues. Protoplasma 229: 209-214.

Sack M, Paetz A, Kunert R, Bomble M, Hesse F, Stiegler G, Fischer R, Katinger H, Stoeger E, Rademacher T (2006) Functional analysis of the broadly neutralizing human anti-HIV-1 antibody 2F5 produced in transgenic BY-2 suspension cultures. FASEB J 21: 1655-1664.

Schmale K, Rademacher T, Fischer R, Hellwig S (2006) Towards industrial usefulness – cryo-cell-banking of transgenic BY-2 cell cultures. J Biotech 124: 302-311.

Yusibov V, Rabindran S, Commandeur U, Twyman RM, Fischer R (2006) The potential of plant virus vectors for vaccine production. Drugs in R&D 7: 203-217.

2005

Bruell D, Bruns CJ, Yezhelyev M, Huhn M, Müller J, Ischenko I, Fischer R, Finnern R, Jauch KW, Barth S (2005) Recombinant anti-EGFR immunotoxin 425(scFv)-ETA’ demonstrates anti-tumor effect against disseminated human pancreatic cancer in nude mice. Int J Mol Med 15: 305-313.

Drakakaki G, Marcel S, Glahn RP, Lund EK, Pariagh S, Fischer R, Christou P, Stoger E (2005) Endosperm-specific co-expression of recombinant soybean ferritin and Aspergillus phytase in maize results in significant increases in the levels of bioavailable iron. Plant Mol Biol 59: 869–880.

Kuepper MB, Huhn M, Spiegel H, Ma JKC, Barth S, Fischer R, Finnern R (2005) Generation of human antibody fragments against Streptococcus mutans using a phage display chain shuffling approach. BMC Biotechnol 5: 4.

Ma JKC, Barros E, Bock R, Christou P, Dale PJ, Dix PJ, Fischer R, Irwin J, Mahoney R, Pezzotti M, Schillberg S, Sparrow P, Stoger S, Twyman RM (2005) Molecular farming for new drugs and vaccines. EMBO Rep 6: 593-599.

Ma JKC, Chikwamba R, Sparrow PAC, Fischer R, Mahoney R, Twyman RM (2005) Plant-derived pharmaceuticals – the road forward. Trends Plant Sci 10: 580-585.

Nicholson L, Gonzalez-Melendi P, van Dolleweerd C, Tuck H, Ma JKC, Fischer R, Christou P, Stoger E (2005) A recombinant multimeric immunoglobulin expressed in rice shows assembly-dependent subcellular localization in endosperm cells. Plant Biotech J 3: 115-127.

Nölke G, Schneider B, Fischer R, Schillberg S (2005) Immunomodulation of polyamine biosynthesis in tobacco plants has a significant impact on polyamine levels and generates a dwarf phenotype. Plant Biotech J 3: 237-247.

Paetz A, Sack M, Thepen T, Tur MK, Bruell D, Finnern R, Fischer R, Barth S (2005) Recombinant soluble human Fc receptor I with picomolar affinity for immunoglobulin G. Biochem Biophys Res Commun 338: 1811-1817.

Ruthardt N, Gulde N, Spiegel H, Fischer R, Emans N (2005) Four-dimensional imaging of transvacuolar strand dynamics in tobacco BY-2 cells. Protoplasma 225: 205-215.

Schiermeyer A, Schinkel H, Apel S, Fischer R, Schillberg S (2005) Production of Desmodus rotundus plasminogen activator a1 (DSPAa1) in tobacco is hampered by proteolysis. Biotechnol Bioeng 89: 848-858.

Schillberg S, Twyman RM, Fischer R (2005) Opportunities for recombinant antigen and antibody expression in transgenic plants - technology assessment. Vaccine 23: 1764-1769.

Schinkel H, Schiermeyer A, Soeur R, Fischer R, Schillberg S (2005) Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells. Transgenic Res 14: 251-259.

Sorrentino A, Schillberg S, Fischer R, Rao R, Porta R, Mariniello L (2005) Recombinant human tissue transglutaminase produced into tobacco suspension cell cultures is active and recognizes autoantibodies in the serum of coeliac patients. Int J Biochem Cell Biol 37: 842-852.

Stöcker M, Klockenbring T, Huhn M, Nachreiner T, Wicklein D, Petersen A, Bauer R, Goerlich R, Fischer R, Barth S (2005) Antigen-specific targeting and elimination of EBV transformed B cells by allergen toxins. J Allergy Clin Immunol 116: 910-915.

Stoger E, Ma JKC, Fischer R, Christou P (2005) Sowing the seeds of success: pharmaceutical proteins from plants. Curr Opin Biotech 16: 167-173.

Stoger E, Sack M, Nicholson L, Fischer R, Christou P (2005) Recent progress in plantibody technology. Curr Pharm Des 11: 2439-2457.

Twyman RM, Schillberg S, Fischer R (2005) The transgenic plant market in the pharmaceutical industry. Expert Opin Emerg Drugs 10: 185-218.

Uhde K, Fischer R, Commandeur U (2005): Expression of multiple foreign epitopes presented as synthetic antigens on the surface of potato virus X particles. Arch Virol 150: 327-340.

Memberships and professional development

Member of the German Society for Immunology (since 2002)

Member of the American Society for Biochemistry and Microbiology (since 1999)

Member of the European Commission Evaluation Expert Panel (since 1996)

Member of the Studienstiftung des deutschen Volkes, Germany (since 1983)

Member of the Society of Biological Chemistry (Germany) since 1986.

Member of the Protein Society, U.S.A. (since 1989)

Member of the Global Research Alliance for Health (since 2004)

Member of the European Plant Science Organisation (since 2001)

Reviewer
FEBS-Letters, FASEB J., J. of Plant Physiology, Molecular Breeding, Plant Molecular Biology, Immunotechnology, J. of Immunological Methods, BBA, Transgenic Research, BioTechniques, Protein Science, Virology, Archives of Virology, J. of Biotechnology, Biotechnology & Bioengineering, Chemical Engineering and Processing, Trends in Biotechnology, Trends in Biochemical Sciences, Vaccine, Plant Journal, Planta Medica, Nature Biotechnology, Cellular and Molecular Biology.


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